SiRNA for targeted inhibition of EGFL9 gene expression, siRNA plasmid, lentivirus, construction method of lentivirus, and application of SiRNA, siRNA plasmid and lentivirus
A technology of gene expression and lentivirus, which is applied in the field of genetic engineering, can solve the problem that there is no research report on the role of expression level, and achieve the effect of inhibiting the ability of invasion and migration
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[0034] Figure 1 to Figure 10 Shown are the embodiments of siRNA, siRNA plasmid, interfering lentivirus and its construction method and application for targeting and inhibiting the expression of EGFL9 gene of the present invention, by studying the expression level of EGFL9 in liver cancer tissue, designing the siRNA sequence of human EGFL9 gene, and The corresponding siRNA plasmid and interference lentivirus were constructed, and the lentivirus-mediated siRNA method was used to specifically inhibit the expression of the EGFL9 gene in liver cancer cells, thereby achieving the effect of inhibiting the invasion and migration of liver cancer cells.
[0035] 1. Real-time quantitative PCR detection of the expression level of EGFL9 gene in liver cancer tissues and cell lines
[0036] 1) Collection of specimens: A total of 20 fresh specimens of liver cancer surgically resected in Guangzhou Red Cross Hospital between March 2012 and April 2015 were collected. All specimens were confirme...
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