SiRNA for targeted inhibition of EGFL9 gene expression, siRNA plasmid, lentivirus, construction method of lentivirus, and application of SiRNA, siRNA plasmid and lentivirus

A technology of gene expression and lentivirus, which is applied in the field of genetic engineering, can solve the problem that there is no research report on the role of expression level, and achieve the effect of inhibiting the ability of invasion and migration

Inactive Publication Date: 2019-06-11
广州市红十字会医院
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Problems solved by technology

However, so far, there is no research report on the expression level of EGFL9 in malignant tumors such as liver cancer and its role in the invasion and metastasis of malignant tumors.

Method used

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  • SiRNA for targeted inhibition of EGFL9 gene expression, siRNA plasmid, lentivirus, construction method of lentivirus, and application of SiRNA, siRNA plasmid and lentivirus
  • SiRNA for targeted inhibition of EGFL9 gene expression, siRNA plasmid, lentivirus, construction method of lentivirus, and application of SiRNA, siRNA plasmid and lentivirus
  • SiRNA for targeted inhibition of EGFL9 gene expression, siRNA plasmid, lentivirus, construction method of lentivirus, and application of SiRNA, siRNA plasmid and lentivirus

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Embodiment Construction

[0034] Figure 1 to Figure 10 Shown are the embodiments of siRNA, siRNA plasmid, interfering lentivirus and its construction method and application for targeting and inhibiting the expression of EGFL9 gene of the present invention, by studying the expression level of EGFL9 in liver cancer tissue, designing the siRNA sequence of human EGFL9 gene, and The corresponding siRNA plasmid and interference lentivirus were constructed, and the lentivirus-mediated siRNA method was used to specifically inhibit the expression of the EGFL9 gene in liver cancer cells, thereby achieving the effect of inhibiting the invasion and migration of liver cancer cells.

[0035] 1. Real-time quantitative PCR detection of the expression level of EGFL9 gene in liver cancer tissues and cell lines

[0036] 1) Collection of specimens: A total of 20 fresh specimens of liver cancer surgically resected in Guangzhou Red Cross Hospital between March 2012 and April 2015 were collected. All specimens were confirme...

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Abstract

The invention discloses an siRNA, an siRNA plasmid and a lentivirus for targeted inhibition of EGFL9 gene expression, as well as a construction method of the lentivirus, and application of the SiRNA,the siRNA plasmid and the lentivirus in preparation of liver cancer treatment drugs, and provides an action target of the EGFL9 gene for liver cancer treatment by researching the expression level of the EGFL9 in liver cancer tissues. An siRNA sequence for targeted inhibition of expression of the EGFL9 gene, and corresponding siRNA expression plasmids and siRNA lentiviruses are designed; therefore,the expression of the EGFL9 gene in liver cancer cells can be efficiently inhibited, the invasion and migration capability of the liver cancer cells can be effectively inhibited, and the EGFL9 gene targeted therapeutic drug is of great significance to the preparation of the EGFL9 gene targeted therapeutic drug for liver cancer; meanwhile, the method can be used for developing corresponding targeted therapeutic drugs such as RNAi, monoclonal antibodies and small molecule antagonists, so that an effective method is provided for developing targeted therapeutic drugs for treating liver cancer byaiming at the EGFL9.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to siRNA, siRNA plasmid, interfering lentivirus and its construction method for targeting and inhibiting the expression of EGFL9 gene and its application in the preparation of liver cancer therapeutic drugs. Background technique [0002] Hepatocellular Carcinoma (HCC; referred to as liver cancer) now ranks fourth in the incidence of malignant tumors in my country, and its annual mortality rate has reached 23.31 / 100,000, ranking second in the mortality rate of malignant tumors in my country, seriously threatening the people of our country. life and health. Although the surgical resection rate of liver cancer has increased significantly in recent years, the treatment methods for liver cancer have become more diversified, and liver transplantation is increasingly used in the treatment of liver cancer, but the overall treatment level of liver cancer has not been significantly...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/867C12N15/66A61K31/713A61P35/00
Inventor 吴帆王百林严伦戴丽冰
Owner 广州市红十字会医院
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