176results about How to "Improve clinical treatment effect" patented technology

The invention discloses a diagnosis marker combination suitable for early screening and diagnosis of lung cancer. The diagnosis marker combination comprises one of 14 serum (or plasma) metabolites ormore: Acylcarnitine C8, decenoyl-carnitine (Acylcarnitine C10: 1), decanoyl-carnitine (Acylcarnitine C10), lauryl-carnitine (Acylcarnitine C12), dimyristoyl-carnitine (Acylcarnitine C14: 2); lysophosphatidyl-ethanolamine (16: 0) (LPE(16: 0)), lysophosphatidyl-ethanolamine (20: 4) (LPE (20: 4)), lysophosphatidyl-ethanolamine (20: 3) (LPE (20:3)), lysophosphatidyl-choline (20: 3 / 0: 0) (LPC (20: 3 / 0:0)), lysophosphatidyl-choline (0: 0 / 22: 5) (LPC (0: 0 / 22: 5)); 3-hydroxyl lauroleic acid (FA(12: 1(OH))), 3-hydroxy myristic dienoic acid (FA(14: 2(OH))), 3-hydroxyl linoleic acid (FA (18: (OH))), 3-hydroxy oleic acid (FA (18: 1(OH))). The diagnosis marker can be used for constructing a diagnosis model, and the model is high in sensitivity and good in specificity, can be used for early screeningand diagnosis of lung cancer, and has good clinical use and popularization values.

The present invention relates to a protein A immunoadsorption material for purifying blood and its preparation method. Said protein A immunoadsorption material is a high-molecular material which is obtained by using agarose gel as carrier matrix, making said agarose gel be reacted with diglycidyl ether coupling reagent to obtain active carrier, then making said active carrier and protein A implement coupling reaction. Said invention can be used for making clinical immunoadsorption therapy.

The invention discloses a bicyclo-ethanol submicron emulsion and a preparation method thereof. the preparation method comprises the steps of dissolving bicyclo-ethanol and emulsifying agent in an oil phase, adding an assistant for emulsifying agent, a stabilizing agent, other additive and a water phase, adopting a cutting dispersing and high-pressure homogeneous emulsification process to prepare an oil-in-water(O / W) submicron emulsion with the average grain diameter below 500 nm and drug loading dosage between 0.01mg / ml and 5mg / ml. The bicyclo-ethanol submicron emulsion is injected through vein and used for treating medium and serious hepatitis. The prepared submicron emulsion does not contain solubilizer such as Tween-80 or organic solvent, can be mixed with glucose injection, physiological saline or distilled water according to random proportion, and can not easily generate insoluble particulates when stored, used or matched with other components, thereby having high security and good stability. The invention also relates to various preparations of the bicyclo-ethanol submicron emulsion.

The invention discloses a miRNA marker in a serum exosome related to early diagnosis of lung adenocarcinoma and application. The marker is selected from hsa-miR-4516, hsa-miR-342-3p, hsa-miR-199a-3p, hsa-miR-150-5p, hsa-miR-140-3p, hsa-miR-18a-5p, hsa-miR-222-3p, hsa-miR-27a-3p, hsa-miR-29a-3p, hsa-miR-221-3p, hsa-miR-500a-3p, hsa-miR-6126 and hsa-miR-6715a-3p. The marker can effectively separate early lung adenocarcinoma cases from healthy people. The marker and probes thereof can be used for preparing a lung adenocarcinoma early diagnosis kit. The marker has an assisting effect on early diagnosis of lung adenocarcinoma.

The invention relates to a staphylococcal protein A (SPA) immunoadsorption material used for blood purification and a preparation method thereof. The invention discloses a macromolecule material which is coupled by agarose gel and SPA. The material is prepared by taking the agarose gel as carrier substrate which is reacted with epoxy bromopropane to obtain the epoxy-based active carrier; after that, polyamines reagent is taken as a space arm which is then reacted with carbonyl diimidazole and is then coupled with the SPA. The material has the advantages of short synthesis time, safe preparation, strong specificity of product, high adsorption efficiency and good regeneration performance to immunoglobulin and the compound thereof, and being able to be applied to clinic immunoadsorption cure.

The invention relates to a compound liquorice tablet and a preparation process thereof. The compound liquorice tablet comprises the following raw materials: liquorice root extracts, opium powder, camphor, star anise oil, sodium benzoate, an adsorbent and a corrective. The preparation process comprises the following steps: adding the adsorbent in the raw material composition, and hiding special foul smell of the camphor and the star anise oil by adopting an adsorption method to eliminate the foul smell; adding the corrective in the raw material composition to enable the compound liquorice tablet to have satisfactory mouthfeel. Therefore, the compound liquorice tablet prepared by the invention is suitable for being taken orally, excellent in mouthfeel, good in clinical effects, and high in bioavailability. Meanwhile, through the improvement on the process, especially, a manner of performing direct tabletting on powder is adopted, so that the compound liquorice tablet is remarkably improved in process level, stable in quality and stable in effect; the production cost of the product is further reduced, and the tablet can serve patients better.

The invention discloses a pancreatic ductal adenocarcinoma marker and a screening method thereof, which belongs to the field of clinical test and diagnosis. In view of the problem that the detection sensitivity and the detection specificity of the existing pancreatic ductal adenocarcinoma diagnosis marker are poor, serum of an early patient of early stage pancreatic duct adenocarcinoma is subjected to a trace metabolomics analysis by the high-performance liquid chromatography-tandem mass spectrometry technology, and different metabolites between normal human and the early stage pancreatic ductal adenocarcinoma patients are found. The different metabolites between normal human and pancreatic ductal adenocarcinoma patients are further analyzed by this technique to find the specific differentmetabolites C10:1 acyl carnitine and lysophosphatidyl choline LysoPC (14:0) of pancreatic ductal adenocarcinoma patients caused by cancer, i.e., the diagnostic molecules of the pancreatic ductal adenocarcinoma. According to the pancreatic ductal adenocarcinoma marker and the screening method thereof, the method can assist CA19-9 in diagnosing pancreatic duct adenocarcinoma patients, and can improve the diagnosis rate for the CA19-9 negative patients by 85%. The method is suitable for the screening of tumor markers.

The shape memory alloy microwound intervertebral fusion device for fixing vertebral column of human body in the course of surgical operation. Said fusion device is a hollow cylinder, between upper portion and lower portion of external side of said hollow cylinder a ring of vertical ribs is set, on the vertical rib the ring barbed teeth rings are set from top to bottom, the vertical rib and the ring barbed teeth ring are crossed to form small hle, and two vertical ribs and parallelly arranged and formed into motion combination surface, the upper end of said fusion device is equipped with ring arranged teeth, and the lower end of said fusion device is equipped with sealing cap on which the cap fastener is set, and the external side of lower bottom edge of said fusion device is equipped with fastener seam, and said cap fastener is fastened on the fastener seam.

The invention belongs to the biotechnical field and relates to a plasma miRNA marker for diagnosis of lung cancer and application. The invention provides the plasma miRNA marker for diagnosis of lung cancer. The marker comprises miRNA-486, miRNA-150, miRNA-205 and miRNA-210. The invention further provides primer probes shown in sequences SEQ ID NO. 1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4 as well as application of the primer probes in preparation of kits for diagnosis of lung cancer. The marker provided by the invention has the beneficial effects that a biomarker which has a relatively high diagnostic value on lung cancer is found, and the biomarker has a relatively high diagnostic value (AUC is 0.975, and the sensitivity and specificity are respectively 90.9% and 100%).

The invention discloses an upper jaw Le Fort I type osteotomy operation guide plate and a design method thereof; the operation guide plate is used for fixing 4 titanium plates on the front wall of the upper jaw so as to rectify cranio-jaw-facial deformity; the guide plate comprises a guide plate body and guide holes; the guide plate body comprises a left structure and a right structure mutually separated; the left and right structures are respectively used for operation of the left upper jaw and right upper jaw; the outer surface of each structure is provided with an osteotomy line and a plurality of guide holes; the osteotomy line is provided with a plurality of osteotomy line mark holes penetrating the structure; a positioning hole is arranged on each guide hole and the structure; one ends, close to the nose cavity, of the two structures are respectively provided with a piriform aperture edge limit composition; a spina nasalis anterior limit composition is arranged below the piriform aperture edge limit composition; the inner surface of the structure is provided with a water guide slot. The invention also provides a method designing the upper jaw Le Fort I type osteotomy operation guide plate.

The invention discloses a gene group evaluating breast cancer molecule typing and a detection kit thereof. The gene group evaluating breast cancer molecule typing is a gene group composed of 216 genes. The invention also discloses the detection kit for the gene group evaluating breast cancer molecule typing. The detection kit comprises a primer capable of detecting the genes in the gene group for evaluating breast cancer molecule typing and cancer recurrence risk. An Illumina second-generation sequencing technology DASL-Seq is employed for detecting expression spectrum of cancer tissue, and on the above basis, breast cancer cases of Chinese women are divided into five molecule typing: 1) luminal type A, 2) luminal type B, 3) HER2 enrichment type, 4) basal cell type and 5) normal-cell like type, and propagation and immunization index are combined for calculating recurrence risk and pridicting recurrence possibility in 10 years, and thus individual treatment on breast cancer is guided.

The present invention discloses a breast cancer diagnostic marker combination and application and a determination method thereof, and particularly relates to 13 difference metabolites (Aspartate, Glutamate, 5-Oxoproline, Cystine, Glutamine, Cysteine, Hypotaurine, Asparagine, Glycerolphosphate, Glycerophosphocholine, Arachidonate, Glyoxylate and pentosidine) and a combination thereof as a breast cancer early detection and diagnosis marker and application thereof and a determination method of the diagnosis marker, and the determination method is a metabonomic analysis method based on liquid / gas chromatography-mass spectrometry of plasma / serum of a patient with breast cancer. The diagnostic marker combination has the characteristics of high sensitivity and high specificity, has high sensitivity and specificity for early diagnosis of breast cancer, can be used for early detection of breast cancer, can gain time for patients to begin treatment as soon as possible, and improves clinical therapeutic effects.

The invention belongs to the field of biological separation engineering and relates to a protein A immune adsorption material used for purifying blood and a preparation method thereof. The invention discloses a biopolymer separating material coupled with agarose gel microspheres and recombinant protein A. An immune adsorption material with high protein A absorption performance is prepared according to the following steps: taking agarose gel as a carrier, activating with allyl glycidyl ether, coupling with sulfydryl on recombinant protein A amino terminal (C terminal) cysteine, and finally, blocking the terminal, thereby fixing the C terminal of the protein A on the agarose gel microspheres at a fixed point. The material has the advantages that the recombinant protein A on the agarose gel microspheres is uniform in structure and fixed in sequence, so that the adsorption capacity of the adsorption material for an antibody is promoted, and the adsorption material can be used for clinical immune adsorption treatment.

The invention relates to the biotechnology area, and the spinal metastatic cancer prognosis judgment is the key to correct determine clinical development strategy, and in the invention, for the present spinal metastatic cancer related molecular biology prognosis judgment evaluation lacking problem, it constructs the spinal metastatic cancer prognosis related molecular marker tissue chip. By screening the clinical data and determining the random data spinal metastatic cancer pathological wax block to produce the spinal metastatic cancer tissue chip; selecting a variety of molecular markers and tissue chips for immunohistochemistry or in-situ hybridization, and through image analysis and quantitative measurement to analyze the expression instance of each molecular marker in the spinal metastatic cancer pathological specimens, and to analyze the relationship between the molecular markers expression and the patients prognosis, and screening the molecular markers which are closely related to the spinal metastatic cancer prognosis. The tissue chip provides a high-throughput, large sample, rapid detection tool for screening the spinal metastatic cancer prognostic related molecular markers, and provides a theoretical basis for the rational determination of the spinal metastatic cancer development strategies.

The invention relates to the technical field of blood purification materials and in particular discloses a high carrying capacity protein A immune adsorption material and a preparation method thereof.The protein A immune adsorption material is a high polymer material prepared from hydrophilic gel microspheres and a protein A through coupling; the hydrophilic gel microspheres are adopted as a carrier medium of the material, after being activated with epoxy chloropropane or oxidized with sodium periodate, the carrier medium reacts with ethidene diamine, and then an amino carrier is prepared; the obtained amino carrier reacts with bis-glycidyl ether to generate an activated carrier; and finally the activated carrier is coupled with the protein A. The material is high in content of the protein A in a coupled manner, high in adsorption efficiency on immune globulin and compounds thereof, and is applicable to clinical immune adsorption treatment.

The invention discloses a manufacture method of a titanium or titanium alloy laryngotracheal interventional stent, comprising the following steps of: 1, determining the structure and the size of a plane spread plate; 2, forming by means of laser engraving: under the protection of inert gas, carrying out laser engraving on the plate to be treated by a laser engraving machine; 3, machining the plane spread plate; 4, deslagging; 5, carrying out heat setting, wherein the treatment process comprises the following steps of: 501, putting into a mould: putting the deslaged-plane plate into a forming cavity of the pre-machined thermal treatment mould; and 502: thermally treating; 6, blasting sand: blasting sand to the thermally-treated laryngotracheal interventional stent by a sand-blasting machine; and 7, ultrasonically cleaning. The manufacture method disclosed by the invention is simple in process step, reasonable to design, convenient to realize, and good in use effect, the oxidization of the stent in the process of machining can be effectively avoided, and the prepared interventional stent is high in dimensional accuracy and good in mechanical property.

The present invention discloses polyglycol treated rotavirus antibody intestine targeting preparation as one kind of antibody medicine and its synthesis process. The novel medicine preparation is prepared through the following steps: first synthesizing the polyglycol activated intermediate MePEG-S-NHS and MePEG-S-EDC; then purifying specific anti-rotavirus antibody RV-IgY; and final performing structural modification of the specific anti-rotavirus antibody with the polyglycol activated intermediate MePEG-S-NHS and MePEG-S-EDC to prepare the polyglycol treated rotavirus antibody intestine targeting preparation. The medicine has certain intestine targeting and positioning effect, raised bioavailability, reduced toxic side effect of original medicine, certain delayed function and enhanced clinical treating effect. The medicine has obvious effect on infantile diarrhea caused by rotavirus.

The invention discloses enrofloxacin sustained-release tablets for pets and a preparation process for the same. The enrofloxacin sustained-release tablets disclosed by the invention are stable in medicine release and safe in medication, as well as is composed of enrofloxacin, a sustained-release or controlled-release material, a filler, an adhesive, a lubricant and a flavouring agent. The enrofloxacin sustained-release tablets prepared by the preparation process disclosed by the invention have the characteristics of being convenient to administrate, lasting in action and stable in curative effect.

The invention discloses a cyclovirobuxinum D crystal with a molecular formula of C27H50N2O2 belonging to monoclinic crystal system and space group P2(1). The process for preparing crystalline consists of dissolving the market available cyclovirobuxinum D into organic solvent, carrying out solution growth by the mode of recrystallization, wherein the solvent include at least 80 wt% of methanol. The invention also discloses two typeso of cyclovirobuxinum D crystal.

The invention discloses a nucleic acid drug delivery system and a preparation method thereof. A pH-sensitive cationic liposome capable of wrapping or loading a nucleic acid drug is prepared by screening lipid components and proportions. The prepared cationic liposome is uniform in particle size, high in drug encapsulation efficiency, stable in system and capable of improving the targeted deliveryefficiency of the nucleic acid drug.

The invention provides a set of genes for head and neck squamous cell carcinoma (HNSCC) molecular typing. The set of genes comprises TP53 gene, CDKN2A gene, FAT1 gene, CASP8 gene, AJUBA gene, PIK3CA gene, NOTCH1 gene, KMT2D gene, NSD1 gene, HLA-A gene, HRAS gene, FBXW7 gene, RB1 gene, PIK3R1 gene, TRAF3 gene, NFE2L2 gene, CUL3 gene and PTEN gene. The invention also provides application of the genes in preparing a kit and gene chip for HNSCC molecular typing, and a kit and gene chip for HNSCC molecular typing prepared by using the genes. The genes can enhance the HNSCC typing rationality and accuracy, thereby providing key technical supports for implementing early diagnosis, effective interception and individualized treatment on HNSCC.

The invention discloses a compound florfenicol preparation for treating swine respiratory tract infection diseases and a preparation method thereof. The compound florfenicol preparation comprises florfenicol, flunixin meglumine, a composite organic solvent and an antioxidant agent, and the florfenicol and the flunixin meglumine are used as main drugs. The compound florfenicol preparation has anti-infection, anti-inflammation, antipyresis and analgesia effects, is dosed through subcutaneous injection and has remarkable effects for preventing and treating the swine respiratory tract diseases. The compound florfenicol preparation is exquisite in preparing process, controllable in quality, safe, reliable, convenient to use, low in cost, and suitable for being popularized and applied to cultivation industry.

The invention provides a bionic immune adsorbent using PAMAM (polyamidoamine) as a spacer arm. The bionic immune adsorbent has the following chemical structure of Gel-PAMAM-AA as shown in the specification, wherein Gel represents a hydroxy-containing hydrophilic carrier; AA represents amino acid; n is 0, 1, 2 or 3; and PAMAM is polyamidoamine dendrimer, the hydrophilic carrier is agarose, cellulose or polyvinyl alcohol, and the amino acid is L-histidine, phenylalanine or tryptophan. The invention also provides a preparation method of the bionic immune adsorbent. By using the dendric macromolecular PAMAM as a spacer arm, the outer surface of the carrier has more active sites for coupling more ligands; by adjusting the position and direction of a triazole ring, the triazole ring is farther from the dendric PAMAM, so that adsorption of target substances is facilitated, and the adsorbent has specific adsorption; and the cost is low, the process is simple, and the reaction condition is mild.

The invention relates to the technical field of medical biological detection, and particularly relates to serum miRNA markers suitable for early screening and diagnosis of ossification of the posterior longitudinal ligament and an application thereof in preparation of a diagnostic reagent or kit for ossification of the posterior longitudinal ligament. The biological markers miRNA-563, miRNA-196b, miRNA-10a and miRNA-129 having relatively high diagnosis value on ossification of the posterior longitudinal ligament are found out for the first time. Through development and application of the serum miRNA markers and the diagnostic kit, early screening of ossification of the posterior longitudinal ligament can be more convenient and easier to implement, the foundation is laid for clinicians to quickly and accurately grasp patient conditions and for improving the clinical treatment effect, and help is provided for finding out novel small-molecular drug targets having potential therapeutic value.

The invention relates to a long-acting naltrexone implant and a preparation method thereof, which belong to the field of pharmaceutical preparations. The long-acting naltrexone implant comprises a polylactic acid and naltrexone, and is characterized in that the viscosity coefficient of the polylactic acid is 0.5-2.5dl / g (chloroform, 30 DEG C); the ratio of the polylactic acid to the naltrexone is1:(0.5-1.8) in parts by weight; the composition structure of the long-acting naltrexone implant is a particle tabletted and coated structure; coated particles are slow release pellets which can be sieved by a 20-60 mesh screen in particle size; the slow release pellets comprise the polylactic acid and the naltrexone; the ratio of the polylactic acid to the naltrexone in the slow release pellets is 1:(1-2) in part by weight; and a coating layer is a DL-polylactic acid. The long-acting naltrexone implant has a good curative effect, better drug-loading rate and better drug release completeness, and has small side effect on human bodies.

The invention discloses a berberine hydrochloride and malic acid eutectic compound as well as a preparation method, a composition and application thereof. Specifically, the invention discloses a novelberberine hydrochloride and malic acid eutectic substance which takes berberine hydrochloride as a medicinal active component and malic acid as a eutectic precursor, a preparation method of the berberine hydrochloride and malic acid eutectic substance, and application of the berberine hydrochloride and malic acid eutectic substance as a medicinal active ingredient in preparation of medicines forpreventing and treating cardiovascular diseases, resisting viruses and cancers, reducing blood fat, reducing blood sugar, resisting inflammation, resisting bacteria and resisting infection.

The invention relates to a sarafloxacin hydrochloride sustain-released injection and a preparation method thereof, belongs to a medicinal preparation field. The sarafloxacin hydrochloride sustain-released injection is composed of sarafloxacin hydrochloride, an emulsifier, a suspending agent, a stabilizing agent, an antioxidant, plant oil for injection, and injection water. The preparation method comprises the following steps of: heating sieved sarafloxacin hydrochloride raw material drug, the plant oil for injection, the emulsifier and the antioxidant in a constant temperature magnetic stirrer, to obtain a mixture A; heating the suspending agent, a water-soluble emulsifier, the stabilizing agent and the injection water in the constant temperature magnetic stirrer, to obtain a mixture B; continuously adding the mixture A into the mixture B, mixing and shearing to obtain colostrums; complementing margin of the colostrum with injection water, then homogenizing, loading, and disinfecting with cobalt 60 ray, to obtain the product. The sarafloxacin hydrochloride injection with obvious sustain-released effect on intramuscular injection of livestock and poultry, can reduce stress reaction, mitigates culture labor intensity, and raises economic benefits and social benefits for livestock and poultry culture.

The invention relates to a sophocarpidine pH / magnetic dual-sensitive hydrogel globule which is orally administrated and is used for treating digestive cancers and the preparation method of the sophocarpidine pH / magnetic dual-sensitive hydrogel globule. The preparation method comprises the steps of: adding 0.3 to 0.7 g of CaCl to chitosan acetum; agitating to dissolve CaCl; preparing a 1.5 to 2.5% sodium alginate solution based on the mass to the volume concentration; weighing a proper amount of sophocarpidine and a proper amount of Fe3O4 nanoparticle respectively, and adding to the sodium alginate solution to obtain a suspension containing the sophocarpidine, the Fe3O4 nanoparticle and the sodium alginate; and weighing the suspension containing the sophocarpidine, the Fe3O4 nanoparticle and the sodium alginate, and dropping to the mixed solution of the chitosan and CaCl2 for crosslinking, and then filtering, washing, and then placing and naturally drying at room temperature so as to obtain the sophocarpidine pH / magnetic dual sensitive hydrogel globule.

The invention discloses a preparation method for a rhizoma bletillae polysaccharide gastric-floating tablet and an application of the rhizoma bletillae polysaccharide gastric-floating tablet and belongs to the technical field of new uses of drugs. The invention relates to a new formulation of rhizoma bletillae polysaccharide, and the new formulation of the rhizoma bletillae polysaccharide is used for treating a gastric ulcer. The gastric-floating tablet is prepared through matching the rhizoma bletillae polysaccharide, xanthan gum and glyceryl behenate by the preparation method disclosed by the invention. The rhizoma bletillae polysaccharide gastric-floating tablet has the characteristics that the preparation method is simple, and a treatment effect on a peptic ulcer is good.

The invention provides application of fangji root extract and fangchinoline in pharmacy. Fangji root extract and fangchinoline are used as components of an acetylcholinesterase inhibitor, and the acetylcholinesterase inhibitor is used for preparing a medicine for preventing and treating Alzheimer disease. The invention provides the application of fangji root extract and fangchinoline in pharmacy,provides a new drug candidate source for preventing and treating Alzheimer disease, and has important significance in the aspects of improving the clinical treatment effect of the Alzheimer disease and reducing the toxic and side effects of drugs.