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Blood purifying protein A immunoadsorption material and synthesizing method thereof

An immunoadsorption material and blood purification technology, which is applied in the field of protein A immunoadsorption material and its synthesis for blood purification treatment, can solve the problems of large batch-to-batch quality differences, increased industrialization costs, and unstable performance of adsorption materials, and achieve Improve the clinical treatment effect, improve the binding efficiency, and have good regenerative performance

Active Publication Date: 2008-06-04
GUANGZHOU KONCEN BIOSCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these two methods eliminate the highly toxic substance cyanogen bromide, and the materials prepared by them are safer to use, but the preparation process requires 10 to 20 hours of end-capping reaction and double Bond reduction is obtained, the reaction time is long, and the cost of industrialization is increased
And due to the complex reaction, the product quality of the adsorption material varies greatly between batches, and the performance is unstable

Method used

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  • Blood purifying protein A immunoadsorption material and synthesizing method thereof
  • Blood purifying protein A immunoadsorption material and synthesizing method thereof
  • Blood purifying protein A immunoadsorption material and synthesizing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Synthesis of Active Sepharose 6FF Agarose Gel Containing Epoxy Groups

[0038] Add 1 liter of Sepharose 6FF agarose gel, 1500 milliliters of 2.5 mol / L NaOH aqueous solution, 3 grams of sodium borohydride in a 5-liter reactor, mix well, add about 300 mL of epibromohydrin, and place In a constant temperature shaker, react at 30° C. for 4 hours. After the reaction was completed, the gel was filtered and rinsed with a large amount of distilled water until neutral. Store the activated medium at 4°C for later use. The number of epoxy groups in the gel activated by this method was detected by the sodium thiosulfate method, and it was measured that there were at least 50 μmol of epoxy active groups per milliliter.

Embodiment 2

[0040] Synthesis of active Sepharose 6FF agarose gel containing amino groups

[0041] 1. Reaction of epoxy-active Sepharose 6FF agarose gel with diethylenetriamine

[0042] In the 5L reactor, add 1 liter of epoxy-active Sepharose 6FF agarose gel synthesized in "Example 1", 1.5L of borate buffer solution of 0.1mol / L, and the pH value is controlled in the scope of 7.0~9.0, Add 200mL of diethylenetriamine and react at a constant temperature of 50°C for 2 hours. After the reaction stopped, rinse with 1mol / L sodium chloride solution and sterile water to remove residual diethylenetriamine to obtain active Sepharose 6FF agarose gel containing amino groups.

[0043] 2. Reaction of epoxy-active Sepharose 6FF agarose gel with ethylenediamine

[0044] In the 5L reactor, add 1 liter of epoxy-active Sepharose 6FF agarose gel synthesized in "Example 1", 1.5L of borate buffer solution of 0.1mol / L, and the pH value is controlled in the scope of 7.0~9.0, Add 160 mL of ethylenediamine, and r...

Embodiment 3

[0048] Synthesis of Sepharose 6FF Agarose Gel Containing Carbonylimidazole

[0049] 1 liter of amino-active Sepharose 6FF agarose gel synthesized in "Example 2" (1, 2 or 3) was successively treated with 25%, 50%, 75%, 100% (v / v) of anhydrous dioxane The ring gradient solution was rinsed, and the gel was gradually transferred from the aqueous phase to anhydrous dioxane solvent, and then added to a 5L reactor, followed by 1500ml of anhydrous dioxane solvent, 60 g of N,N'- Carbonyldiimidazole was reacted at a temperature of 20°C for 2 hours. After stopping the reaction, rinse with 100%, 75%, 50%, 25% (v / v) dioxane gradient concentrated solution and distilled water at 4°C successively, and gradually transfer the carrier to the water phase to obtain carbonyl imidazolyl Sepharose 6FF agarose gel, proceed to the next reaction immediately.

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Abstract

The invention relates to a staphylococcal protein A (SPA) immunoadsorption material used for blood purification and a preparation method thereof. The invention discloses a macromolecule material which is coupled by agarose gel and SPA. The material is prepared by taking the agarose gel as carrier substrate which is reacted with epoxy bromopropane to obtain the epoxy-based active carrier; after that, polyamines reagent is taken as a space arm which is then reacted with carbonyl diimidazole and is then coupled with the SPA. The material has the advantages of short synthesis time, safe preparation, strong specificity of product, high adsorption efficiency and good regeneration performance to immunoglobulin and the compound thereof, and being able to be applied to clinic immunoadsorption cure.

Description

technical field [0001] The invention belongs to medical biomaterials, and in particular relates to protein A immunoadsorption materials used for blood purification treatment and a synthesis method thereof. Background technique [0002] The occurrence and development of many diseases are due to the accumulation of pathogenic factors in the body, such as excessive accumulation of triglycerides, cholesterol, lipids, etc. in the body can cause hyperlipidemia and a series of cardiovascular and cerebrovascular diseases A series of symptoms of autoimmune diseases such as systemic lupus erythematosus (SLE), idiopathic thrombocytopenic purpura, and myasthenia gravis are all caused by the appearance of some autoantibodies in the body. Malignant tumor cells produce some immunosuppressive complexes, which inhibit the normal immune response of the body. On the one hand, the cancer cells escape the body's immune system and survive; on the other hand, the immune function of the body decrea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22
Inventor 陈校园张旭锋汪志刚李光吉许春生
Owner GUANGZHOU KONCEN BIOSCI
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