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Composition with Staphylococcus aureus resisting effect and preparation method and application thereof

A staphylococcus and composition technology, applied in the field of traditional Chinese medicine composition, can solve the problems of antibiotic accumulation and antibiotic residue, achieve the effect of extending the industrial chain, reducing content, reducing breast acinar hyperplasia and acinar inflammatory cell infiltration

Active Publication Date: 2020-04-24
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In clinical practice, long-term low-dose antibiotic infusion and immersion are often used for prevention and treatment, resulting in antibiotic residues in dairy products and further leading to the accumulation of antibiotics in humans

Method used

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  • Composition with Staphylococcus aureus resisting effect and preparation method and application thereof
  • Composition with Staphylococcus aureus resisting effect and preparation method and application thereof
  • Composition with Staphylococcus aureus resisting effect and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A preparation method of a composition with an anti-staphylococcus aureus effect, comprising the following steps:

[0038] (1) Take the medicinal material of Sophora flavescens, pulverize it, add 10 times the amount of 95% ethanol, heat and reflux 3 times, combine the extracts, concentrate under reduced pressure to obtain Sophora flavescens extract for later use;

[0039] (2) Take the licorice medicinal material, pulverize it, add 10 times the amount of 95% ethanol, heat and reflux 3 times, combine the extracts, concentrate under reduced pressure to obtain the licorice extract for later use;

[0040] (3) Add the dehydrated alcohol of its weight 5 times of volume to step (1) Sophora flavescens extractum, after stirring and dissolving, get supernatant and transfer to D-101 macroporous adsorption resin column, wash away water-soluble and unadsorbed components, then eluted with ethanol with a volume concentration of 80%, and the eluent was concentrated under reduced pressure...

Embodiment 2

[0045] A preparation method of a composition with an anti-staphylococcus aureus effect, comprising the following steps:

[0046] (1) Take flavescens medicinal residues, put them in an open glass column, add 10 times the amount of ethyl acetate to soak overnight, collect the percolation liquid at a flow rate of 3ml / min, then add 6 times the amount and 3 times the amount in turn soaked in ethyl acetate for 12h, and collected the leachate at a flow rate of 5ml / min and 8ml / min successively, combined the leachate, concentrated under reduced pressure to obtain Sophora flavescens extract for subsequent use;

[0047] (2) Take licorice residue, put it in an open glass column, add 10 times the amount of ethyl acetate to soak overnight, collect the percolation liquid at a flow rate of 3ml / min, and then add 6 times and 3 times the amount of Soak in ethyl acetate for 12 hours, and collect the leachate at a flow rate of 5ml / min and 8ml / min successively, combine the leachate, concentrate und...

Embodiment 3

[0053] A preparation method of a composition with an anti-staphylococcus aureus effect, comprising the following steps:

[0054] (1) Take Sophora flavescens, crush it, put it in an open glass column, add 10 times the amount of ethyl acetate to soak overnight, collect the percolation liquid at a flow rate of 3ml / min, then add 6 times the amount and 3 times A small amount of ethyl acetate was soaked for 12 hours, and the leachate was collected at a flow rate of 5ml / min and 8ml / min successively, the leachate was combined, concentrated under reduced pressure to obtain Sophora flavescens extract, and set aside;

[0055] (2) Take the licorice medicinal material, crush it, put it in an open glass column, add 10 times the amount of ethyl acetate to soak overnight, collect the percolation liquid at a flow rate of 3ml / min, and then add 6 times the amount and 3 times the amount in turn Soak in ethyl acetate for 12h, and collect the leachate at a flow rate of 5ml / min and 8ml / min successiv...

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Abstract

The invention discloses a composition with a Staphylococcus aureus resisting effect. The composition consists of general flavone of sophora flavescens and general flavone of liquorice. The general flavone parts of sophora flavescens and liquorice are prepared through extraction, extraction, enrichment and the like, and are compounded. The results of in-vitro research shows that the composition directly inhibits the growth of gram-positive bacteria such as Staphylococcus aureus and can reduce the formation of biofilms and the content of staphyloxanthin; the results of in-vivo studies prove thatthe composition can obviously reduce mammary gland alveolar hyperplasia and alveolar inflammatory cell infiltration of mice caused by Staphylococcus aureus infections, and significantly reduce the bacterium carrying amount of mouse mammary gland tissues of each model group and the content of IL-1 beta, IL-2 and TNF-alpha inflammatory factors in the mammary gland tissues. According to the invention, the comprehensive utilization of medicinal materials including sophora flavescens and liquorice and the quality and efficiency improvement of the industry can be realized; the waste is turned intothe wealth; and the composition has good economic effects and social benefits, and accords with the sustainable development strategy in China.

Description

technical field [0001] The invention relates to a traditional Chinese medicine composition, in particular to a composition with active ingredients of Sophora flavescens and licorice for preventing and treating Staphylococcus aureus infection, especially mastitis, its preparation method and application. Background technique [0002] Sophora flavescens was first recorded in "Shen Nong's Materia Medica" and listed as a middle grade. It is the dried root of Sophora flavescens Ait., a plant of the genus Sophora flavescens. It has the effects of clearing away heat and dampness, killing insects, and diuresis. , Jaundice, urinary retention, eczema, eczema, skin itching, mange and leprosy. Glycyrrhiza licorice was first recorded in "Shen Nong's Materia Medica" and listed as top grade. It is the dry root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. Tonifying qi, clearing away heat and detoxification, eliminating phlegm and relieving cough, relieving spasm and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/489A61P31/04A61P15/14
CPCA61K36/484A61K36/489A61K2236/333A61K2236/39A61K2236/53A61K2236/55A61P15/14A61P31/04A61K2300/00
Inventor 魏丹丹段金廒曹雅琦郭盛赵明曾飞
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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