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Application of a flavonoid compound in snow chrysanthemum and its pharmaceutical composition in the preparation of medicines for treating acute pancreatitis

A kind of acute pancreatitis, compound technology, application in snow chrysanthemum flavonoid compound and its pharmaceutical composition in the preparation of acute pancreatitis medicine application field

Active Publication Date: 2022-02-08
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant reports on the treatment of pancreatitis with the extract of snow chrysanthemum or the flavonoids isolated from it at home and abroad.

Method used

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  • Application of a flavonoid compound in snow chrysanthemum and its pharmaceutical composition in the preparation of medicines for treating acute pancreatitis
  • Application of a flavonoid compound in snow chrysanthemum and its pharmaceutical composition in the preparation of medicines for treating acute pancreatitis
  • Application of a flavonoid compound in snow chrysanthemum and its pharmaceutical composition in the preparation of medicines for treating acute pancreatitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Preparation and Identification of Flavonoids in Example 1 Snow Chrysanthemum

[0050] 1. Weigh 5 kg of dried snow chrysanthemum, add 25 L of 95% ethanol, and extract under reflux for 3 times at 80° C. for 3 hours each time. The extract is concentrated under reduced pressure to obtain 580 g of extract.

[0051] 2. Mix the 580g extract obtained in step 1 with 580g polyamide, carry out column chromatography through a polyamide column, and use water, 35% ethanol and 65% ethanol as mobile phases to elute successively, as shown in Table 1 As shown, the eluate XP2 obtained by elution with 35% ethanol was concentrated to obtain a 35% ethanol extraction fraction (60.5 g, XP2).

[0052] The elution condition of table 1 polyamide chromatographic column (eluent is 4-5 retention volumes)

[0053] eluent Elution volume eluent water 20L XP1 35% ethanol 20L XP2 65% ethanol 20L XP3

[0054] 3. The 35% ethanol extraction fraction (60.5 g, XP2) ob...

Embodiment 2

[0063] Example 2 Protective effect of flavonoids on pancreatic acinar cell necrosis induced by sodium thiocholate in vitro

[0064] After the male Balb / c (20-25g) mice were killed by dislocation, the pancreatic tissue was taken, washed twice with HEPES solution, and collagenase was injected into the pancreatic tissue along the pancreatic duct with a syringe. Put the pancreatic tissue into 1mL of collagenase, place it in a water bath at 37°C for 18-20min, suck out the pancreas, put it into 4mL HEPES solution, pipette, suck out and filter, then pipette in turn, filter with a 100μM filter, and centrifuge at 700rpm After 2 minutes, pour out the upper liquid completely, add 4-6ml HEPES solution to resuspend, and mix well to obtain the experimental cells. The cells were equally divided into blank group, model group and different dosage administration groups, 1 mL in each group.

[0065] The drug is directly prepared with normal saline, and diluted with freshly prepared HEPES before...

Embodiment 3

[0067] Example 3 Improvement of local and systemic symptoms of cerulein-induced acute pancreatitis mouse model by flavonoids.

[0068] 1) Model establishment

[0069] Male Blab / C mice were randomly divided into groups, and fasted for 12 hours before the experiment. Intraperitoneal injection of cerulein 50μg / kg, 7 consecutive injections, each interval of 1h.

[0070] 2) Animal grouping and handling

[0071] Thirty male Blab / C mice were randomly divided into five groups (N=6): normal control group, acute pancreatitis group, and three dose groups of compound 1 (12.5, 25 and 50 mg / kg).

[0072] Normal control group: intraperitoneal injection of normal saline at the same dose as cerulein solution, 7 consecutive injections with an interval of 1 hour between each injection. The first injection of cerulein was recorded as 0h, and the same amount of solvent as the test drug was injected at 0, 4 and 8h.

[0073] Acute pancreatitis group: intraperitoneal injection of cerulein, 7 cons...

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Abstract

The invention discloses the application of a flavonoid compound in snow chrysanthemum and its pharmaceutical composition in the preparation of medicine for treating acute pancreatitis. The invention provides an application of a flavonoid compound in the preparation of a medicine for treating acute pancreatitis. The flavonoids can significantly improve the necrosis of pancreatic acinar cells, and protect the local and systemic symptoms of two kinds of acute pancreatitis. The present invention will fill the medical value of snow chrysanthemum and the flavonoids isolated therein in the protection of pancreatitis, and provide new diagnosis and treatment methods and application prospects for clinical screening and / or preparation of drugs for the treatment of acute pancreatitis. a new choice.

Description

technical field [0001] The invention relates to the application of a flavonoid compound in snow chrysanthemum and its pharmaceutical composition in the preparation of medicine for treating acute pancreatitis. Background technique [0002] Acute pancreatitis (AP) is one of the common clinical acute abdomen, its etiology and pathogenesis are complex, the mortality rate remains high, reaching 30% to 60%, and various serious complications are prone to occur. Currently, acute pancreatitis is defined as an inflammatory disease characterized by acute inflammation and pancreatic parenchymal necrosis, and is divided into mild acute pancreatitis, moderately severe acute pancreatitis, and severe acute pancreatitis according to its severity. The pathogenic factors of acute pancreatitis are complex, including common biliary tract diseases, excessive alcohol intake, hyperlipidemia, drugs and surgical trauma. The pathogenesis of acute pancreatitis has not yet been elucidated. The early th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P1/18A61P29/00C07H17/07C07H1/08
CPCA61K31/7048A61P1/18A61P29/00C07H17/07C07H1/08
Inventor 杜丹黄伟胡立强夏庆金涛梁鸽
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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