194 results about "Goblet cell" patented technology

An objective of the present invention is to provide a method of testing for bronchial asthma or chronic obstructive pulmonary disease, a method of screening for candidate compounds for treating bronchial asthma or chronic obstructive pulmonary disease, and a pharmaceutical agent for treating bronchial asthma or chronic obstructive pulmonary disease. The present invention identified genes whose expression levels varied between respiratory epithelial cells that had been stimulated by IL-13 to induce the goblet cell differentiation, and unstimulated respiratory epithelial cells. The respiratory epithelial cells were cultured according to the air interface method. The genes were revealed to be useful as markers for testing for bronchial asthma or chronic obstructive pulmonary disease and screening for therapeutic agents for such diseases. Specifically, the present invention provides methods of testing for bronchial asthma or chronic obstructive pulmonary disease and methods of screening for compounds to treat the diseases based on the comparison of the expression levels of marker genes identified as described above.

Hypersecretion of mucus in the lungs is inhibited by the administration of an epidermal growth factor receptor (EGF-R) antagonist. The EGF-R antagonist may be in the form of a small organic molecule, an antibody, or portion of an antibody that binds to and blocks the EGF receptor. The EGF-R antagonist is preferably administered by injection in an amount sufficient to inhibit formation of goblet cells in pulmonary airways. The degranulation of goblet cells that results in airway mucus production is thereby inhibited. Assays for screening candidate agents that inhibit goblet cell proliferation are also provided.

Hypersecretion of mucus in the lungs is inhibited by the administration of an epidermal growth factor receptor (EGF-R) antagonist. The EGF-R antagonist may be in the form of a small organic molecule, an antibody, or portion of an antibody that binds to and blocks the EGF receptor. The ESGF-R antagonist is preferably administered by injection in an amount sufficient to inhibit formation of goblet cells in pulmonary airways. The degranulation of goblet cells that results in airway mucus production is thereby inhibited. Assays for screening candidate agents that inhibit goblet cell proliferation are also provided.

A composition for preventing or treating an eye disease includes adiponectin as an active ingredient. Adiponectin as an active ingredient is eventually revealed to show prevention or therapeutic efficacies for eye diseases such as dry eye (syndrome), inflammatory eye disease and side effects due to the use of contact lenses by promoting tear secretion, alleviating ocular surface irregularities, decreasing inflammatory cytokines on the ocular surface and lacrimal gland, and increasing conjunctival goblet cell density. In addition, the composition having eye contact lubrication effects may be used as cleaners or lubricants for preventing non-bacterial inflammation due to wearing contact lenses.

A method of making a live cell construct is carried out by: (a) providing a non-cellular support having a top surface and a bottom surface, (b) contacting live undifferentiated cells to the non-cellular support, and then (c) propagating a gastrointestinal epithelial cell monolayer on said top surface. In some embodiments, the live cells in the monolayer include: (i) undifferentiated cells (e.g., stem or progenitor cells); and (ii) optionally, but in some embodiments preferably, differentiated cells (e.g., enterocytes, Paneth cells, enteroendocrine cells, tuft cells, microcells, intra-epithelial lymphocytes, and/or goblet cells). Constructs formed by such methods and methods of using the same (e.g., in high through-put screening) are also described.

The invention discloses an application of novel eye drops in treatment of dry eyes. The novel eye drops contain alpha-MSH; the mass concentration of the alpha-MSH is 10<-4> to 10<-3>mg/ml; the novel eye drops are used for treating the dye eyes; and the optimized mass concentration of the alpha-MSH is 10<-4>mg/ml. By using the novel eye drops, experiment analysis on the breakup time of a tear film (BUT), schirmer test, fluorescein staining, grade and tear ferns is carried out on rats; an overall eyeball of each rat is extracted to be subjected to HE and periodic acid-Schiff (PAS) staining; and the fresh cornea of each rat is extracted to be subjected to real-time quantitative PCR detection of expression levels of interleukin-1beta and tumor necrosis factor-alpha m RNA in corneal tissues, so that the experiments prove that the eye drops containing alpha-MSH are capable of promoting schirmer in rats, prolonging the breakup time of the tear film, stabilizing the tear film, promoting corneal epithelium damage repair and goblet cell quantity repair, and relieving ocular surface inflammation, and are favorable for relieving lesions of the dry eyes.

The invention discloses a feed additive capable of improving an intestinal function of grass carps and application of the feed additive. An effective component of the additive is glycylglutamine; and the adding amount in grass carp feed is 0.25-0.75%. With the adoption of the additive disclosed by the invention, the growth and fat metabolism of the grass carps can be effectively facilitated, and the oxidation resistance of the grass carps is improved; a shape structure of small intestines of the grass carps can be improved; villus height, crypt depth, mucous membrane thickness, the quantity of goblet cells and the quantity of lymphocytes of the intestines are increased; and the secretion of an intestinal digestive enzyme can be accelerated, and the feed additive has active improvement effect on the intestinal function of the grass carps.

The invention discloses lactobacillus rhamnosus FLRH93. The lactobacillus rhamnosus FLRH93 is preserved in the Guangdong Microbial Culture Collection Center, and has a preservation number of GDMCC No:60956. Animal damage intervention experiment results show that the lactobacillus rhamnosus FLRH93 can obviously improve the intestinal injury caused by drugs; tissue staining sections show that the strain maintains the integrity of a small intestine structure, reduces the loss of goblet cells after drug treatment, and can regulate and control the secretion of Bcl-2 and NLRP3, thereby reducing generation of inflammatory factors IL1-beta and TNF-alpha. The invention belongs to the technical field of microorganisms. The strain provided by the invention can improve the survival rate of mice withdrug injury and can also remarkably promote healing of wound skin.

The invention encompasses isolation, culture and characterization of goblet cells in vitro form mammalian conjuctiva. Goblet cells can be cultured from conjunctiva of such mammals as, e.g., humans, rats, mice, rabbits and the like. In another aspect of the invention, the culture of goblet cells has a concentration of pure goblet cells of 10% or greater.

The invention relates to a preparation method of a recombinant conjunctival epithelial diaphragm containing goblet cells, which induces differentiation of goblet cells by a gamma-secretase inhibitor. The preparation method provided by the invention takes autologous conjunctiva or allogenic conjunctiva as a raw material for culturing and preparing the recombinant conjunctival epithelial diaphragm containing goblet cells. Compared with the original preparation methods of conjunctival epithelial diaphragms, the culture method provided by the invention can promote proliferation of conjunctival epithelial cells at the early stage, so that the formed conjunctival epithelial multiple layers are more uniform; an amniotic membrane nested type culture mold is used as a culture support, the culture surface of the amniotic membrane is flat, the differentiated goblet cells exist among the conjunctival epitheliums, have functions and are convenient in clinical application, and the amniotic membrane is not liable to rupture; and the density and ability to secrete mucoprotein of the goblet cells in the recombinant conjunctival epithelial diaphragm can also be increased, thus more meeting the clinical demand.

The present invention relates to a pharmaceutical composition for preventing or treating dry eye, the pharmaceutical composition including, as an active component, a novel peptide is disclosed, wherein it is confirmed that the peptide has effects on improving tear production and corneal surface smoothness for dry eyes induced by desiccation stress and suppressing detachment of corneal epithelial cells, reduction in conjunctival goblet cells, and generation of inflammatory factors, thereby applying a composition including the peptide as an active component to the pharmaceutical composition for preventing or treating dry eye.

The invention discloses application of tetrahydrocurcumin in treating allergic asthma. The therapeutic action of the tetrahydrocurcumin (THC) on allergic asthma is firstly found and proved; from the perspective of immunity, the THC can be beneficial to alleviating nose allergy and itching, inhibiting lung eosinophilic granulocyte infiltration, reducing lung goblet cell grume generation, reducing lung collagen deposition, and inhibiting Th2 cell factor production, can provide beneficial information for clinic disease evaluation, and has a favorable application prospect in asthma treatment.

The invention discloses a dendrobium officinale composition and a preparation method and application thereof.The dendrobium officinale composition is prepared from, by weight, 10-50 parts of dendrobium officinale and 5-30 parts of radix asparagi, the medicine components are mixed and smashed, a certain amount of water is added, the medicine components are soaked for a period of time and subjected to reflux extraction, an extracting solution is filtered, filtrate is subjected to vacuum concentration, and a traditional Chinese medicine composition extract is obtained. The composition disclosed by the invention has a relatively good prevention and treatment effect on ulcerative colitis, and can be used for remarkably reducing the disease activity score (DAI score) of a mouse with ulcerative colitis, increasing the colon length, improving the histological lesions such as colonic mucosa injury, inflammatory cell infiltration, goblet cell and mucus reduction and the like, and preventing and treating the ulcerative colitis. One of the ways for preventing and treating ulcerative colitis is to increase the abundance of UCG-007 bacteria in intestinal flora. The traditional Chinese medicine composition has the characteristics of simple formula and exact curative effect, and has better effect, lower cost and good market development and application prospects compared with single use of dendrobium officinale.