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Lactobacillus reuteri 22 and application thereof

A technology of Lactobacillus reuteri and probiotics, applied in the directions of Lactobacillus, application, bacteria, etc., can solve the problems of poor adhesion, only passing bacteria, unable to fully exert physiological functions, etc., to promote healthy development, enhance The effect of intestinal immunity and enhancement of intestinal mucosal immune function

Active Publication Date: 2019-12-06
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The various probiotic properties of lactic acid bacteria are based on the adhesion to intestinal epithelial cells. If the adhesion ability is poor or cannot adhere, they can only be passing bacteria and cannot fully exert their physiological functions.

Method used

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  • Lactobacillus reuteri 22 and application thereof
  • Lactobacillus reuteri 22 and application thereof
  • Lactobacillus reuteri 22 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Sample source and preliminary screening of strains, probiotic properties and identification of strains

[0031] 1. Source of samples and preliminary screening of strains

[0032] The samples were from healthy chickens. After slaughter, the abdominal cavity was opened, and the two ends of the duodenum were tied up with sterile cotton rope. After cutting, they were immediately put into pre-cooled saline and sent to the laboratory for strain and bacteria separation within 30 minutes. The operation is carried out in an ultra-clean workbench. The intestinal segment is cut longitudinally with sterile scissors, then the contents on the surface of the intestine are washed with sterile PBS buffer (pH 7.4, 0.01M), and then the intestine is gently scraped with a blade The wall mucosa is about 0.5g, placed in a triangular conical flask filled with glass beads and shaken thoroughly. After standing at room temperature for 2-3min, 100μl of suspension is applied to the surface of ...

Embodiment 2

[0046] Example 2 Detection of the effect of oral Lactobacillus reuteri 22 on the intestines of chicks

[0047] Select newborn healthy 3-day-old white-feather broilers (weight about 44g), 20 per group, divided into blank group and control group. The blank group chicks were orally administered with sterile 200μl PBS (pH7.4, 0.01M) for 7 days; Roy 22 groups of Lactobacillus seri, each chick was continuously inoculated with 200μl of 10 9 CFU / ml Lactobacillus reuteri 22, sampling on the 8th day. The content of lipopolysaccharide (LPS) in the serum was detected by ELISA method, and the inflammatory factors TNF-α and IL-1β in jejunum tissue were also detected. image 3 The results showed that compared with the negative control Con group, the levels of inflammatory factors decreased slightly after treatment with Lactobacillus reuteri 22 ( image 3 A-C), weight has a tendency to increase ( image 3 E), the expression level of tight junction protein related genes increased ( image 3 F-G), ...

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Abstract

The invention discloses a lactobacillus reuteri 22 and application thereof. The lactobacillus reuteri 22 disclosed by the invention is separated from healthy chicken flocks, is classified and named aslactobacillus reuteri, and has a preservation number of CGMCC No.17932. By detecting the performance of probiotics, the invention finds that the lactobacillus reuteri 22 has good acid resistance, cholate resistance and adhesion performance, and can inhibit growth of pathogenic bacteria. Through Animal assays, in a healthy physiological state, the lactobacillus reuteri 22 does not influence the level of inflammatory factors, but can increase the number of goblet cells and promote the increase of expression levels of related genes such as compact protein, defensin and lysozyme, can increase thelength of intestinal villi and the depth of crypt, and has the potential of maintaining intestinal mucosa barriers. Therefore, the strain is expected to be developed into probiotics for maintaining achicken intestinal mucosa barrier and guaranteeing green and healthy chicken breeding.

Description

Technical field [0001] The present invention relates to biological prevention and control technology, in particular to a strain of Lactobacillus reuteri 22 and its application in chicken breeding. Oral administration of Lactobacillus reuteri 22 can enhance intestinal immune function, promote the proliferation of intestinal epithelium, and maintain Potential for homeostasis of the intestinal barrier. Background technique [0002] Lactobacillus belongs to Finnicutes, Bacilli, Lactobacillales, and Lactobacillaceae in taxonomy. It is a Gram-positive bacillus that does not form Spores can produce lactic acid after breaking down sugar. Lactobacillus is a normal bacteria in the intestines of humans and animals. It is closely related to the absorption of nutrients by the host and the development of the intestinal mucosal immune system. [0003] The various probiotic properties of lactic acid bacteria are established on the basis of adhesion to intestinal epithelial cells. If the adhesion...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/75C12R1/225
CPCC12N1/20A23K10/18A23K50/75C12R2001/225C12N1/205A23V2400/173
Inventor 庾庆华谢霜赵世义李昱辰杨倩
Owner NANJING AGRICULTURAL UNIVERSITY
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