Feed additive capable of improving intestinal function of grass carps and application of feed additive
A technology for feed additives and intestinal function, which is applied in the field of aquatic product feed additives, and can solve problems such as intestinal atrophy, slow growth of grass carp, and decreased digestion and absorption capacity
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experiment example 1
[0018] Experimental Example 1: Effects of Glycylglutamine (Gly-Gln) on Growth Performance and Antioxidative Immunity of Grass Carp
[0019] Test materials: Glycylglutamine was purchased from Beijing Dingguo Changsheng Biotechnology Co., Ltd., white powder with a purity of 98%.
[0020] The test location: the cage culture base of Chetianjiang Reservoir, Xinhua County, Hunan Province.
[0021] Experimental feed and production: Feed raw materials are crushed and passed through a 60-mesh sieve, and trace ingredients are uniformly mixed by step-by-step expansion method. After mixing, pellet feed with a particle size of 1.5mm-2.0mm is extruded with a feed pellet machine, and air-dried in a cool place Store at -20°C for later use. The test feed was divided into a control group and a glycylglutamine added group (the feed group of the present invention). The feed group of the present invention is to replace 2.5 grams of secondary powder in the control group with 2.5 grams of glycyl...
Embodiment 2
[0030] Example 2: Effects of Glycyl Glutamine on Blood Physiological and Biochemical Indexes of Grass Carp ring
[0031] Test site, test fish, test feed and feeding method are all described in Example 1. After the breeding experiment, 10 grass carp with similar specifications were randomly selected from each cage, and blood was collected from the tail vein with a 1ml syringe. After being refrigerated overnight at 4°C, centrifuged at 3500r / min for 15min, the upper serum was taken for detection of serum biochemical components.
[0032] The test results are shown in Table 3. As can be seen from Table 3, after grass carp is fed with the feed of the present invention adding glycyl glutamine, the content of total cholesterol, super antioxidant dismutase and catalase in serum is significantly lower than that of the control group, while urea nitrogen, glutathione Glycerin, glutathione peroxidase and total antioxidant capacity were significantly higher than those in the control grou...
Embodiment 3
[0035] Example 3: Effects of Glycylglutamine on Grass Carp Intestinal Morphology and Function
[0036] Test site, test fish, test feed and feeding method are all described in Example 1. After the breeding experiment, 3 grass carp were randomly selected from each cage, placed in an ice tray and quickly dissected, and the foregut was washed with 0.86% saline, fixed in Bouin's solution for 24 hours, and paraffin sliced for measurement and analysis. Calculate the height of intestinal villi, depth of crypts, thickness of mucosa, number of goblet cells, and number of lymphocytes; randomly select 6 grass carp from each box, take the foregut, mash it, homogenize it in a water bath, centrifuge and take the supernatant to measure the intestinal Trypsin, lipase and amylase.
[0037] The test results are shown in Table 4. As can be seen from Table 4, after grass carp is fed with the feed of the present invention adding glycyl glutamine, the height of intestinal villi, the depth of cry...
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