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Construction method and application of acute pancreatitis cell model

A technology for acute pancreatitis and cell models, applied in pancreatic cells, artificial cell constructs, detection of programmed cell death, etc., can solve the problem of increasing the time of acute pancreatitis cell models, increasing the cost of acute pancreatitis cell models, and the irritant tree frog. It can increase the degree of necrosis of pancreatic acinar cells, reduce the half-maximum effect time, and achieve good reproducibility.

Active Publication Date: 2022-03-29
WEST CHINA HOSPITAL SICHUAN UNIV
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Problems solved by technology

However, the stimulant cerulein used in this method is expensive and difficult to obtain, which increases the cost of establishing an acute pancreatitis cell model; in addition, this method needs to be processed for 24 hours, which takes a long time, which increases the time for establishing an acute pancreatitis cell model

Method used

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  • Construction method and application of acute pancreatitis cell model
  • Construction method and application of acute pancreatitis cell model
  • Construction method and application of acute pancreatitis cell model

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Example 1: Method for Constructing Glucolipid Metabolism Disorder-Related Acute Pancreatitis Cell Model

[0031] 1. Experimental method

[0032] 1. Experimental animals

[0033] SPF-grade male C57BL / 6J mice (7-8 weeks) were purchased from Beijing Huafukang Biotechnology Co., Ltd., license: SCXK (Beijing) 2020-0004. Animals were raised in separate cages in the Experimental Animal Center of West China Hospital of Sichuan University, with 5 animals per cage, constant temperature (25±2°C) and lighting control (12h day / night cycle), free access to food and water, and formal feeding after 1 week of adaptive feeding. Start experimenting. This experiment was approved by the Ethics Committee of the Experimental Animal Center of West China Hospital, Sichuan University, and all animal experiments and related operations were carried out in accordance with school and national standards.

[0034] 2. Freshly isolated mouse pancreatic acinar cells

[0035] Prepare 4-(2-hydroxyethyl...

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Abstract

The invention provides a construction method and application of an acute pancreatitis cell model, and belongs to the field of cell models. It is found for the first time that glucose treatment can significantly increase the number of pancreatic acinus necrosis cells and significantly reduce the half-maximum effect time, and an acute pancreatitis cell model is successfully constructed. It is found for the first time that glucose combined fatty acid treatment can further increase and induce the pancreatic acinus cell necrosis degree, and an acute pancreatitis cell model is successfully constructed. The adopted raw materials are easy to obtain and low in price, and the modeling cost is reduced; according to the invention, the acute pancreatitis cell model can be successfully constructed in a short time, and the modeling time is shortened. The modeling method is convenient and fast, easy to operate, low in cost and good in repeatability. The acute pancreatitis cell model constructed by the invention not only can be used for screening acute pancreatitis treatment drugs, but also can provide a good platform for the occurrence and development mechanism of acute pancreatitis, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of cell models, and in particular relates to a construction method and application of a cell model of acute pancreatitis. Background technique [0002] Acute pancreatitis (AP) is an inflammatory injury such as pancreatic edema, hemorrhage and necrosis caused by the self-digestion of pancreatic tissue caused by various etiologies. The main clinical manifestations are acute and persistent pain in the middle and upper abdomen, accompanied by nausea, vomiting, etc., and the increase of blood amylase or lipase exceeds 3 times the upper limit of the normal value, accompanied by typical imaging changes. The inflammation spreads to the whole body in severe patients. Often associated with local or systemic complications, the prognosis is poor. Characteristic findings of pancreatitis include hyperamylaseemia, abnormal activation of digestive enzymes in the acini (eg, trypsinogen to trypsin), accumulation of large vacuoles in a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12Q1/02
CPCC12N5/0676G01N33/507C12N2500/34C12N2500/36C12N2503/02G01N2500/10
Inventor 杨鑫敏
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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