35 results about "Estradiolum" patented technology

The invention relates to a novel cathode photoelectrochemical method for homogeneous phase detection of 17[Beta]-estradiol using a CuBi<2>O<4> material, and belongs to the field of analysis and detection. An ITO electrode modified by CuBi<2>O<4> is used as a working electrode, methylene blue (MB) or ferric chloride hemin that can be embedded in G-tetrahedra are used as photoelectrochemical signalmolecules, and the specific binding reaction between 17[Beta]-estradiol and aptamers is combined with the rolling circle amplification reaction (RCA) to control the insertion / release of the signal molecules, so that a homogeneous phase cathode photoelectrochemical detection method without biomolecule labeling and without fixing the biomolecules on the electrode surface is constructed. The novel cathode photoelectrochemical method for homogeneous phase detection of 17[Beta]-estradiol using the CuBi<2>O<4> material has high sensitivity and selectivity, with a linear range of 0.05 nmol / L - 10 nmol / L and a detection limit of 2.0 pmol / L.

The invention discloses a preparation method of a biological immunity sensor which is based on functional nano-material construction and has dual signal development strategies of electrogenerated chemiluminescence and photoelectrochemistry, and the prepared sensor is easy to operate, convenient to carry, fast in detection, low in cost and capable of being used for fast and sensitive detection of estradiol in the fields of routine production and life and the like.

The invention discloses a molecularly imprinted polymer test strip used for detecting 17 beta-Estradiolum and a preparing method thereof. The test strip is modified with the molecularly imprinted polymer, and the molecularly imprinted polymer bears specific binding sites of 17 beta-Estradiolum. The specific binding sites can not only carry out specific binding with enzyme marked 17 beta-Estradiolum, but also can carry out specific binding with 17 beta-Estradiolum. The detection is realized through the competitive binding between the 17 beta-Estradiolum to be detected, the enzyme marked 17 beta-Estradiolum and the biding sites. The molecularly imprinted polymer test strip used for detecting 17 beta-Estradiolum can realize the specific binding of the target template module and can magnify signal through the color reaction between enzyme and substrate. The method for detecting 17 beta-Estradiolum does not need complicated sample pretreatment, has simple operation, low cost, and good reliability, and can be used for the detection of 17 beta-Estradiolum in food or clinic.

The invention discloses ethinyl estradiol degrading bacteria and a method for repairing polluted soil thereof. The ethinyl estradiol degrading bacteria belong to Hyphomicrobium with preservation number CCTCC NO: M2017539, and the name of the ethinyl estradiol degrading bacteria is Hyphomicrobium sp. GHH. According to the ethinyl estradiol degrading bacteria and the method for repairing the polluted soil thereof, the ethinyl estradiol degrading bacteria are separated from farmland, and the bacteria can remove ethinyl estradiol in soil; and ryegrass and the ethinyl estradiol degrading bacteria are combined to repair the ethinyl estradiol polluted soil, the degrading bacteria promote the growth of plants and the extraction and enrichment of ethinyl estradiol by the plants, so that the repaireffect of the plants on the ethinyl estradiol polluted soil is significantly improved.

The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a medicine for treating mammary gland hyperplasia (known as breast addiction in traditional Chinese medicine), a preparation method and application thereof. The medicine is prepared from Ashitaba ethanol extract, malt water extract and hawthorn water extract. By comparing the treatment of model rats suffering from mammary gland hyperplasia, the compound medicine of the present invention can effectively: 1. reduce the serum estradiol content and increase the serum progesterone hormone level; The number of hyperplasia; ③ reduce the number of obliterated acini in the lobules of breast tissue; it has a good effect of soothing the liver and eliminating breast addiction.

The invention discloses a preparation method of a humic acid modified Fe2O3 nanomaterial and an application thereof to the removal of estradiol in the technical field of environment functional material preparation. The method comprises the steps: dissolving Fe(NO3)3.9H2O into deionized water; then, adding a KOH solution and an NaHCO3 solution, carrying out a thermal insulation reaction, and then,separating a sediment; and next, mixing a solid with humic acid, regulating the pH, carrying out constant-temperature vibration, and then, carrying out centrifugal separation. The humic acid modifiedFe2O3 nanomaterial prepared by using the preparation method has relatively large specific surface area and is more in adsorption site and relatively strong in adsorption capacity due to various functional groups on the surface.

The invention discloses a strain of laccase-producing fungus, which is named as trachephrine ( Trametes hirsuta ) La‑7, was deposited in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on September 27, 2019. The culture preservation number is CGMCC No 5.2199, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. Utilize the tracheomyces of the present invention ( Trametes hirsuta ) La‑7 fermented extracellular crude laccase can effectively remove 17 β ‑Estradiol.

The invention belongs to the technical field of food safety testing immunoassay and relates to a bisphenol A and 17beta-estradiol detection kit based on an upconversion fluorescence aptamer sensor, anapplication and a detection method. The kit comprises (1) first carboxylated core / shell upconversion nanoparticles NaYF4:Yb,Er@NaYF4:Nd, (2) second carboxylated core / shell upconversion nanoparticlesNaYF4:Yb,Tm@NaYF4:Nd, (3) a target aptamer sequence and a regular tetrahedron structure composition sequence designed according to a complementary strand of the aptamer as well as (4) a black phosphorus nanogold composite dispersion, wherein targets comprise bisphenol A and 17beta-estradiol; the regular tetrahedron structure composition sequence comprises a complementary strand sequence of the aptamer sequence, and the at least part of the complementary strand sequence forms the regular tetrahedron structure sequence. With adoption of the kit or the detection method, bisphenol A and / or 17beta-estradiol can be detected with high sensitivity.

The invention discloses a method for determining an endocrine disrupter in a water body, the method comprises the following steps: (1) taking a water body to be detected, and adding an internal standard substance to obtain a mixed solution; (2) extracting, filtering and purifying the mixed solution to obtain a solution to be detected; and (3) measuring the solution to be measured by using a liquid chromatography tandem mass spectrometer to obtain the content of the endocrine disrupter in the solution to be measured, wherein the endocrine disrupter includes estrone, estradiol, bisphenol A, octyl phenol, nonyl phenol, triclosan and triclocarban. By implementing the method, the content of the endocrine disrupter in the water body can be accurately and efficiently measured, and a good data basis is provided for subsequent effective control of the content of the endocrine disrupter in the water body.

The invention discloses an estradiol production device. The device comprises a reaction kettle, an elutriation kettle, a centrifuge, a concentrating kettle, a decolorization kettle and a drying device, wherein the drying device comprises an outer cylinder, a drying cylinder and an inner cylinder, a motor is arranged on the bottom surface of the outer cylinder, and an exhaust fan blade, a cylinder-type impeller and a vortex impeller are arranged on the rotating shaft of the motor; the exhaust fan blade is disposed between the drying cylinder and the bottom surface of the outer cylinder, the cylinder-type impeller is disposed between the drying cylinder and the inner cylinder, and the vortex impeller is arranged at the bottom of the inner cylinder; an infrared heating tube is arranged on thetop surface of the inner cylinder, an air purifying ring is arranged at the upper end of a sandwich cavity of the outer cylinder, and a material inlet is formed in the center of a top plate; a sliding rod is arranged at the edge of the material inlet, a tray is connected to the lower end of the sliding rod, and a screw rod is arranged on the side edge of the material inlet; a screw sleeve is arranged on the screw rod, a gear ring which is arranged on the screw sleeve is meshed with a drive gear, and the drive gear is connected with a drive motor; and the lower end of the screw rod is connected with the tray, and a cover plate is connected with tops of the sliding rod and the screw rod. Through the estradiol production device, the preparation time of an intermediate product during production of estradiol can be reduced, and rapid high-quality production of estradiol can be realized.

The invention discloses application of 17a alpha-D-homo-ethynylestradiol-3-ethyl ester (E838 for short) to preparation of medicines for treating the hematopoietic function of aplasticanemia (AA) mice and protecting radiation of mouse bone marrow cell chromosome. The E838 is used for treating radiation induced AA test animal models and damage of the mouse bone marrow cell chromosome caused by gamma ray irradiation, so an occurrence and development route for treating the ionizing radiation induced AA of the mice and damage of the mouse bone marrow cell chromosome caused by gamma ray irradiationis established.