Method for determining endocrine disrupter in water body

A technology for endocrine disruptors and a method for determination is applied in the field of determination of endocrine disruptors in water bodies, which can solve problems such as the decline of the nutrient structure of ecosystems, and achieve the effects of accurate determination, high sensitivity and low detection limit.

Inactive Publication Date: 2021-11-12
HAINAN UNIVERSITY
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

EDCs may also have selective effects on specific trophic-level taxa, leading to the decline of ecosystem trophic structure

Method used

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  • Method for determining endocrine disrupter in water body
  • Method for determining endocrine disrupter in water body
  • Method for determining endocrine disrupter in water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] The present embodiment adopts the above-mentioned method to measure the content of endocrine disruption in the Pearl River water body, specifically including:

[0083] (1) Sampling in the Guangzhou section of the Pearl River. Three parallel 1L water samples were collected in clean brown glass bottles at the same sampling point. When collecting water samples, a mixed sample was taken from the three left, middle and right points below the horizontal plane of the same section. Immediately add 50 mL of chromatographically pure methanol and 400 μL of 4 mol / L sulfuric acid solution to the collected water samples, put them in a 4°C ice box for later use, and carry out pretreatment within 48 hours.

[0084] (2) Sample pretreatment

[0085] (2.1) Solid-phase extraction: seven internal standards (E1-d4, E2-d4, BPA-d16, 13 C 12 -TCS, 4-n-NP-2,3,5,6-d4; TCC- 13 C 6 , 4-t-OP- 13 C 6 ) (100ng each) was added to the filtered 1L water sample. Each solid-phase extraction cartridg...

Embodiment 2

[0099] The sample recovery rate, detection limit and quantification limit of the assay method in the present invention were investigated.

[0100] Among them, the standards were added in parallel for 4 times during the determination of the recovery rate, the water sample was ultrapure water, the concentration of 4-NP was 1000ng / L, and the concentration of other EDCs was 100ng / L. Measured separately and calculated the recovery rate.

[0101] The limit of detection (LOD) and limit of quantitation (LOQ) were calculated using 4 times of water samples with a spiked concentration of 5 ng / L, and the standard deviation (Standard derivation, SD) of each compound was calculated, then 3 times SD is LOD, 10 times SD is LOQ. The recoveries and detection limits of the seven EDCs are shown in Table 3.

[0102] Table 3 Recovery rate and detection limit of EDCs

[0103]

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Abstract

The invention discloses a method for determining an endocrine disrupter in a water body, the method comprises the following steps: (1) taking a water body to be detected, and adding an internal standard substance to obtain a mixed solution; (2) extracting, filtering and purifying the mixed solution to obtain a solution to be detected; and (3) measuring the solution to be measured by using a liquid chromatography tandem mass spectrometer to obtain the content of the endocrine disrupter in the solution to be measured, wherein the endocrine disrupter includes estrone, estradiol, bisphenol A, octyl phenol, nonyl phenol, triclosan and triclocarban. By implementing the method, the content of the endocrine disrupter in the water body can be accurately and efficiently measured, and a good data basis is provided for subsequent effective control of the content of the endocrine disrupter in the water body.

Description

technical field [0001] The invention relates to the technical field of water pollution monitoring, in particular to a method for measuring endocrine disruptors in water. Background technique [0002] Endocrine Disrupting Chemicals (EDCs) refer to a kind of exogenous substances, which have high estrogen activity, and micro-exposure can cause endocrine imbalance in organisms; in addition, EDCs can also affect the nervous system, The immune system, etc. produce toxic effects, causing the survival ability of aquatic organisms to decline. Due to the medium-strong lipophilicity of EDCs, in addition to direct toxicity to exposed organisms, they can also accumulate and latent in organisms. When this cumulative effect reaches a certain level, it can cause various toxicities such as oxidative damage and carcinogenesis to organisms. The damage cannot be eliminated in the short term, and may even be irreversible. This cumulative toxic effect can also be transmitted through biomagnific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 王赛王旭王团团王定莹王晓迪
Owner HAINAN UNIVERSITY
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