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Method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold

A nucleic acid aptamer and resonance scattering technology, which is applied in the field of nucleic acid aptamer-modified nano-gold catalytic resonance scattering spectroscopy to detect mercury, achieves the effects of simple equipment, easy operation and strong specificity

Inactive Publication Date: 2010-06-09
GUANGXI NORMAL UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

But there is no nucleic acid aptamer-modified nano-gold catalyzing the reaction of Fehling's reagent-aldehyde. The study of cuprous oxide particle-enhanced resonance scattering spectroscopy and its determination of Hg 2+ report

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  • Method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold
  • Method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold

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Embodiment Construction

[0017] (1) Prepare a test system with a known concentration: Prepare five 5mL graduated test tubes, and pipette 18 μL of 0.17 μmol / L single-stranded oligosaccharide nucleotides (ssDNA, whose sequence is 5'-TTT CTT CTT TCT TCC CCC CTT GTT TGTTGT TT-3') solution, 400μL pH 7.0 concentration is 0.02mol / L NaH 2 PO 4 -Na 2 HPO 4 Buffer solution, 375μL 58μg / mL nano-gold solution, shake well, let stand for 5min; add 1.000×10 -2 mol / LHgCl 2 Standard solution 50, 100, 200, 300, 400μL, then add 22.5μL 2.0mol / L NaCl solution, dilute to 1.5mL, mix well, filter with a filter membrane with a pore size of 150nm, dilute the filtrate 10 times, and make the following For measurement,

[0018] In addition, pipette 69 μL of 0.20mol / L CuSO 4 solution, 75μL 1.23mol / L KNaC 4 h 4 o 6 , the KNaC 4 h 4 o 6 The solution contains 6.25 mol / L NaOH, add 80 μL each of the filtrates 1 to 5 prepared in step (1) to each test tube, then add 900 μL 23.2 mmol / L furfural solution, dilute to 3.0 mL, mix w...

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Abstract

The invention discloses a method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold, which comprises the following steps: Hg2+ aptamer ssDNA and nanogold are combined to form nanogold AussDNA modified by DNA, stable Hg2+aptamer-Hg2+compound is generated by T-T mismatching when Hg2+ and AussDNA coexist, nanogold is gathered under the action of high-concentration NaCl, redundant AussDNA in filtrate after membrane filtration is taken as a nano catalytic seed crystal to catalyze the reaction of Cu2+ and furfural so as to reduce Cu2+ into Cu+, Cu+ reacts with OH- in solution to generate CuOH, and finally generate Cu2O particles. From the above preparation method, a determined system and a reagent blank system of mercury concentration can be known, resonance scattering strength of the two systems are measured at the 605nm part, drawn into a standard curve, and then prepared into a sample detection system, a deltaI sample is solved, the mercury content in the sample is calculated on the standard curve. The invention has the advantages of high sensitivity, strong specificity, wide determination range, simple equipment, and convenient operation, is suitable for determining various samples containing mercury, and provides reliable analysis data for environment monitoring, foods, cosmetics and other fields.From the above method, a determined system and a reagent blank system of which the mercury concentration is known can be prepared, resonance scattering strength of the two systems are measured at the 605nm part and drawn into a standard curve, and then a sample detection system is prepared, delta I sample is calculated, the mercury content in the sample is calculated by the standard curve.The invention has the advantages of high sensitivity, strong specificity, wide determination range, simple equipment, and convenient operation, and is suitable for determining various samples containing mercury.

Description

Technical field: [0001] The invention relates to a special chemical analysis technology, in particular to a method for detecting mercury by nucleic acid aptamer-modified nano-gold catalyzing resonance scattering spectroscopy. Background technique: [0002] Mercury is a chemical substance with severe physiological toxicity. Due to its persistence, easy migration and high bioaccumulation, it has become one of the most concerned environmental pollutants in the world. Soluble mercury ion (Hg 2+ ) is the most stable inorganic mercury, which is corrosive and carcinogenic to cells; mercury ions are converted into methylmercury through the action of microorganisms in the environment, which can be enriched in the human body through the food chain and can cause brain damage or other chronic diseases. Therefore, it is of great significance to establish a method for detecting mercury ions with high sensitivity and high selectivity in environmental monitoring, food safety and medicine. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/63G01N21/47G01N1/28
Inventor 梁爱惠李纪顺蒋治良温桂清刘庆业
Owner GUANGXI NORMAL UNIV
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