52 results about "Methylmercury" patented technology

The invention discloses a methyl mercury visual detection method of gold-mercury alloy nanoparticle growth regulation and control based on a DNA template, and belongs to the technical field of heavy metal detection. According to the method, an HT9 DNA nucleic acid template is uniformly mixed with different concentrations of methyl mercury standard solutions, incubation is carried out for 5 minutesafter mixing, a sodium borohydride solution is added to prepare nano seed crystal by reduction, a hydroxylamine hydrochloride solution and a chloroauric acid solution are sequentially added then, a solution color is recorded after fully and uniformly mixing, a standard color card is established, the absorbance of the solution is determined by a microplate reader and corresponds to the concentration of methyl mercury, and a standard work curve is established. Operation is carried out by replacing the methyl mercury standard solution with a marine product extracting solution, a corresponding solution color is recorded and compared with the standard color card, the absorbance of the solution is detected and substituted into the standard curve, so that organic mercury in a marine product is detected. The method is simple, sensitive, stable and high in anti-interference capability, does not need heating, can be used to complete detection within 30 minutes, and is low in detection cost.

The invention provides an ion imprinted fluorescent sensor for selectively detecting methyl mercury ions and a preparation method for the ion imprinted fluorescent sensor. The sensor is prepared by the following three processes of: (1) forming a pre-copolymer by using the methyl mercury ions, a functional monomer and a fluorescent material through coordination; (2) adding a cross linker and an initiator, and performing initiated polymerization under proper conditions by using a microporous membrane as a supporting medium to immobilize template molecules in the polymer in a complementary spatial structure form; and (3) removing the template molecules from the polymer in an acid or ethylene diamine tetraacetic acid (EDTA) eluting mode, leaving specific holes, and thus obtaining the fluorescent methyl mercury ion imprinted composite membrane sensor. The obtained imprinted composite membrane shows high selectivity and sensitivity to methyl mercury ions, and can be reused for multiple times; and the fluorescent sensor is easy and fast to prepare, and can be used for selective detection of the methyl mercury ions in samples of environment and the like.

The invention belongs to the field of analytical chemistry, and relates to a fluorescent probe for quickly identifying mercury and methylmercury, a preparation method and an application thereof. The structural formula of the fluorescent probe is as follows:. The fluorescent probe prepared by the present invention improves the detection sensitivity without adding any other additional materials, and avoids the addition of additional materials, reduces the consumption of additional materials and reduces the source of errors in the detection; in the detection The imaging of the analyte in living cells and zebrafish has been successfully detected, and it is suitable for real-time monitoring and differentiation of cells and zebrafish in vivo. This method has not been able to do it in the previous method. The realization of imaging has greatly promoted the in-depth study of distinguishing mercury from methylmercury.

The invention discloses a method for detecting methyl mercury ions. This method combines the advantages of nanomaterials and utilizes methylmercury ions to block the formation of silver nanoparticles using fluorescein-labeled T-rich DNA as a template to achieve fluorescence retention, so as to detect methylmercury ions. The method for detecting methyl mercury ions of the present invention is convenient, simple and fast, does not need expensive instruments, has low cost, and has high detection sensitivity, and can achieve high response to methyl mercury ions while better overcoming the interference of divalent mercury ions. good practical application prospects.

The invention was involved in organic mercury lyase merB gene, which was expressed in plants. The gene was achieved by changing the sequence of merB catalysed methyl mercury into ion mercury in the bacteria.Then modified merB gene was transferred into plants so that modified gene plants with strong resistance to organic mercury was achieved. The modified gene plants could be used to degrade pollution of organic mercury in soils.