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Photoacoustic imaging probe, and preparation method and application thereof

A photoacoustic imaging and probe technology, which is applied in the direction of material analysis, instruments, and measurement devices by optical means, can solve the problem of unreported photoacoustic contrast agents, and achieves good application prospects, convenient operation, and simple preparation process. Effect

Inactive Publication Date: 2018-06-26
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no photoacoustic contrast agent for the detection of methylmercury in vivo has been reported.

Method used

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  • Photoacoustic imaging probe, and preparation method and application thereof
  • Photoacoustic imaging probe, and preparation method and application thereof
  • Photoacoustic imaging probe, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] As shown in reaction formula (1), 111 mg of compound 1 with the following chemical formula

[0028]

[0029] (that is, compound 1 in Reaction Formula 1) was dissolved in 5 mL of anhydrous dimethylformamide (DMF, that is, compound 2 in Reaction Formula 1) to prepare a solution, and then 39 mg of 1-(2-aminoethyl) 3- Phenylthiourea (ie compound 2 in Reaction Formula 1) and stirred at 80° C. for 8 h under an argon atmosphere. Then, the DMF solvent was removed by reducing the pressure, and the prepared sample was purified by a silica gel column (DCM / MeOH was 99:1-15:1)), that is, compound 3 in the reaction formula, and finally a dark blue powder sample hCy7 was obtained. Methods The yield of cyanine dye hCy7 was 50%.

[0030]

[0031] Reaction formula 1: wherein,

Embodiment 2

[0033] Mix 1 mg cyanine dye hCy7 with 15 mg liposome (PEG 5000 -DSPE) was dissolved in 1 mL of dichloromethane, then the solution was added to 3 mL of pure water for 120 seconds, and finally the organic solvent was removed by rotary evaporation to obtain a blue suspension, which contained such as figure 1 LP-hCy7 nanoprobes with a particle size of 50-100 nm are shown.

Embodiment 3

[0035] Disperse LP-hCy7 in deionized water to make a 2 mg / mL solution. Add different ion solutions (Ca2+, Mg 2+ , Mn 2+ , Fe 2+ 、Co 2+ 、Ni 2+ , Zn 2+ 、Cu 2+ , MeHg + ) for 30 minutes, and deionized water as a control, test the photoacoustic image and signal value of the solution at 690nm and 860nm respectively, and compare the ratio of the photoacoustic signal value PA860 / PA690 in different groups. Test results such as figure 2 As shown, compared with the control group and other ions, the addition of MeHg + In the solution group, the photoacoustic signal at 860nm increased significantly, while the signal value at 690nm decreased, so that the ratio PA860 / PA690 increased significantly.

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Abstract

The invention provides a photoacoustic imaging probe, and a preparation method and application thereof. According to the invention, liposome and a cyanine dye are subjected to self-assembling to formthe water-soluble nanoprobe, wherein the cyanine dye has specific response to methylmercury. When a solution contains MeHg<+>, the nanoprobe promotes cyclization through mercury so as to form LP-hCy7', results in a decrease in the intensity of an absorption peak at a wavelength of 690 nm and an increase in the intensity of an absorption peak at a wavelength of 860 nm, and correspondingly leads toreduction in photoacoustic signals at the wavelength of 690 nm and substantial strengthening of signals at the wavelength of 860 nm. The content of MeHg<+> in a biological sample can be detected through photoacoustic imaging of a living body and the PA signal ratio of the LP-hCy7 nanoprobe at the wavelengths of 860 nm and 690 nm. The photoacoustic imaging probe is simple in structure; and the preparation method does not need complicated and expensive equipment, and is simple in process, convenient to operate and easy to realize industrial production.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a photoacoustic imaging probe and its preparation method and application. Background technique [0002] Mercury (mercury) is a common heavy metal pollutant that can cause severe water and soil pollution. Methylmercury (MeHg+) is an organic form of mercury and is more toxic than other inorganic salt forms of mercury. Methylmercury is a fat-soluble substance, so it can pass through biomembranes and cause damage to the brain and nervous system of organisms, especially to pregnant women and infants. Methylmercury is easily accumulated in the human body through foods such as fish. At present, the detection of methylmercury mainly includes gas chromatography (GC), high performance liquid chromatography (HPLC), capillary electrophoresis (CE) coupled with specific detection such as atomic fluorescence spectroscopy (AFS), mass spectrometry (MS) or inductively coupled plasma Mass spe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/17
CPCG01N21/1702
Inventor 黄鹏王生林静陈小元
Owner SHENZHEN UNIV
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