Fluorescent probe kit for detecting total mercury and methyl mercury in water and detection method
A technology of fluorescent probes and detection methods, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, measuring devices, etc., can solve the problem of fewer fluorescent probes, and achieve the effect of strong affinity
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Embodiment 1
[0050] S11. Use a pipette gun to draw 2.5mL of acetonitrile-HEPES buffer solution and 2.5mL of standard water sample BY-1 into the cuvette, mix well and irradiate with an ultraviolet lamp with a wavelength of 365nm in a dark place to obtain a reference;
[0051] S12. Add 0.1mL of probe solution to the above reference, mix well and irradiate again in the dark with a UV lamp with a wavelength of 365nm. It can be observed that there is blue fluorescence visible to the naked eye in the cuvette;
[0052] S13. Continue to add 4 μL of DNA solution to the reference in step S12. After mixing, continue to irradiate with an ultraviolet lamp with a wavelength of 365 nm in the dark. It can be observed that the blue fluorescence in the cuvette disappears, and the solution appears similar to the reference. traits.
[0053] It can be seen from the above test phenomena that the standard water sample BY-1 only contains mercury ions.
Embodiment 2
[0055] S21. Use a pipette gun to draw 2.0mL of acetonitrile-HEPES buffer solution and 3.0mL of standard water sample BY-2 into the cuvette, mix well and irradiate in the dark with an ultraviolet lamp with a wavelength of 365nm to obtain a reference;
[0056] S22. Add 0.1 mL of probe solution to the above reference, mix well and irradiate again in a dark place with an ultraviolet lamp with a wavelength of 365 nm, and it can be observed that there is blue fluorescence visible to the naked eye in the cuvette;
[0057] S23. Continue to add 5 μL of DNA solution to the reference in step S22. After mixing, continue to irradiate with an ultraviolet lamp with a wavelength of 365 nm in the dark. It can be observed that the blue fluorescence in the cuvette does not disappear, and there is no change in intensity.
[0058] It can be seen from the above test phenomena that the standard water sample BY-2 only contains methylmercury.
Embodiment 3
[0060] S31. Use a pipette gun to draw 3.0 mL of acetonitrile-HEPES buffer solution and 2.0 mL of standard water sample BY-3 into the cuvette, mix well and irradiate in the dark with an ultraviolet lamp with a wavelength of 365 nm to obtain a reference;
[0061] S32. Add 0.1 mL of the probe solution to the above reference, mix well and irradiate again in the dark with an ultraviolet lamp with a wavelength of 365 nm, and it can be observed that there is blue fluorescence visible to the naked eye in the cuvette;
[0062] S33. Continue to add 5 μL of DNA solution to the reference in step S32. After mixing, continue to irradiate with an ultraviolet lamp with a wavelength of 365 nm in the dark. It can be observed that the blue fluorescence intensity in the cuvette decreases, but it is not completely quenched. , indicating that the standard water sample BY-3 contains mercury ions and some methylmercury. In order to ensure that the fluorescence of mercury ions can be completely quenche...
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