56 results about "Resonance scattering" patented technology

The invention discloses a method of catalytic resonance scattering spectral determination of mercury by using aptamer modified nanogold, which comprises the following steps: Hg2+ aptamer ssDNA and nanogold are combined to form nanogold AussDNA modified by DNA, stable Hg2+aptamer-Hg2+compound is generated by T-T mismatching when Hg2+ and AussDNA coexist, nanogold is gathered under the action of high-concentration NaCl, redundant AussDNA in filtrate after membrane filtration is taken as a nano catalytic seed crystal to catalyze the reaction of Cu2+ and furfural so as to reduce Cu2+ into Cu+, Cu+ reacts with OH- in solution to generate CuOH, and finally generate Cu2O particles. From the above preparation method, a determined system and a reagent blank system of mercury concentration can be known, resonance scattering strength of the two systems are measured at the 605nm part, drawn into a standard curve, and then prepared into a sample detection system, a deltaI sample is solved, the mercury content in the sample is calculated on the standard curve. The invention has the advantages of high sensitivity, strong specificity, wide determination range, simple equipment, and convenient operation, is suitable for determining various samples containing mercury, and provides reliable analysis data for environment monitoring, foods, cosmetics and other fields.From the above method, a determined system and a reagent blank system of which the mercury concentration is known can be prepared, resonance scattering strength of the two systems are measured at the 605nm part and drawn into a standard curve, and then a sample detection system is prepared, delta I sample is calculated, the mercury content in the sample is calculated by the standard curve.The invention has the advantages of high sensitivity, strong specificity, wide determination range, simple equipment, and convenient operation, and is suitable for determining various samples containing mercury.

The invention discloses a preparation method of a nanopore chip with a plasmon resonance scattering response function. The preparation method comprises the following steps: (1) selecting a chip substrate: selecting a silicon chip as a substrate, and depositing an insulating material on the front surface of the silicon chip, without the insulating material on the back surface; (2) etching a square area on the back surface of the silicon chip by a wet etching method until a nanopore chip film is exposed on the back surface; (3) etching nanopores: forming the nanopores on the nanopore chip film by using a focused ion beam or a focused electron beam to bomb the nanopore chip film; (4) modifying noble metal: coating a noble metal layer on the surface of the nanopore chip film by using a magnetron sputtering instrument. The preparation method is simple in steps; the prepared nanopore chip is controllable in size and relatively low in cost; through noble metal modification on the nanopores, the nanopore chip with a photoelectric response function is prepared; the nanopore chip can cause a plasmon resonance coupling phenomenon so as to amplify a spectral signal, and can be applied to many fields such as electrochemical detection of the nanopores.

The invention discloses a catalytic reaction-resonance scattering spectroscopic method for detecting trace molybdenum. The method is characterized by including the following steps: (1) preparing a standard molybdenum system; (2) preparing a blank control system; (3) right amount of components are taken to be arranged in a quartz cell and then are put on a fluoro spectrophotometer to be synchronously scanned (lambdaem is equal to lambdaex), the resonance scattering light intensity I540nm of a 540nm part is detected, the blank value of a blank system is I540nmb, and the expression that delta 540nm is equal to 540nm minus I540nmb is calculated; (4) the delta 540nm is used for making the working curve of the concentration relationship of molybdenum; (5) a detection system is prepared according to the method of step one so as to obtain the delta I540nm of a tested object by calculation; and (6) according to the working curve of step four, the concentration of molybdenum of the tested object is calculated. The method has the advantages of high sensitivity, favorable selectivity, simple operation, high safety, non-toxicity, low-cost reagent needed, etc.

The invention discloses a method for quantitative detection of ultramicro-protein based on the principle of nano gold particle biomineralization, which comprises the following steps: firstly, by taking the first generation of PPIHA dendrimer and chloroauric acid (HAuCl4) as raw materials, preparing nano gold colloid with surface modified by amine by means of a microwave reflux heating method, andcombining the nano gold colloid with to-be-detected protein to form conjugate at a normal temperature; secondly, carrying out biomineralization on the nano gold-protein conjugate in a sodium citrate-chloroauric acid system; and then carrying out resonance scattering spectral analysis on the biomineralization product by using a fluorescence spectrum spectrograph. Because of the linear relation between the peak strength of the resonance scattering spectrum of the product at the characteristic wavelength and the protein concentration, the ultramicro-protein can be quantitatively detected. The method is easy and quick and has high sensitivity, high specificity and high accuracy. In addition, the method has a good portability and can be used for early tumor diagnosis, quick food safety detection, water quality monitoring and the like.

The invention relates to a method for accurately measuring the content of chitosan oligosaccharide according to a resonance rayleigh scattering method, and belongs to the field of macromolecule detection. The chitosan oligosaccharide and rubylith are combined to form ionic associate under the certain condition, then the resonance scattering strength is obviously increased, and lauryl sodium sulfate serving as a sensitizer is added to improve the sensitivity. The resonance scattering strength and the chitosan oligosaccharide form a linear relation within certain concentration range, the linearrelation is taken as a quantitative basis of the chitosan oligosaccharide, and the resonance rayleigh scattering method for measuring the chitosan oligosaccharide is established. The measurement of the method is affected by the ionic concentration and the interferent, a standard product with the ionic concentration controlled to 0.05 mol / L to 0.20 mol / L is taken as a quantitative standard in the actual measurement of a sample, and the method has the advantages of cheap agents, high sensitivity, good repeatability, simplicity and convenience in operation, and the like.

The present invention discloses a simple and rapid immune resonance scattering spectroscopy for the determination of ceruloplasmin, which mainly makes use of the specific immune binding reaction between goat anti-human ceruloplasmin and ceruloplasmin under the condition of PEG in the 37 DEG C water bath for production of immune complex particles. The resonance scattering is existed in the complex particles. The concentration of the ceruloplasmin is linear with the strength of the resonance scattering when the concentration of ceruloplasmin is in a certain range, thereby establishing a novel method for the rapid determination of the ceruloplasmin. The present invention has the advantages that: compared with the prior art, the determination method has simple instrument, easy operation, high sensitivity and selectivity, and easy achievement of the reaction conditions. The dosage of the goat anti-human CER reagent is less and the cost is low.

The invention discloses a surface plasma resonance absorption spectrometry method for determining NH<4+> by using a sodium tetraphenylborate ligand for adjusting and controlling nano-silver catalytic activity. The method is characterized by comprising the following steps: (1) preparing a NH<4+> standard solution system with known concentration; (2) preparing a blank control solution system; (3) calculating according to the equation that delta A is equal to A minus A0; (4) making a working curve according to a relationship between the delta A and NH<4+> concentration; (5) preparing a to-be-measured sample solution, determining the resonance scattering peak intensity value of the to-be-measured sample solution as an A sample, and calculating according to the equation that delta A sample is equal to A sample minus A0; (6) calculating out the NH<4+> content of the sample solution according to the working curve. The method of the invention uses the ligand for adjusting and controlling nano-enzyme catalytic activity, and is simple, convenient, quick and high in sensitivity.

The present invention relates to a high sensitivity assay for molecular typing of a biological sample using surface-enhanced Raman scattering (SERS) including resonance scattering (SERRS); capture probes for capturing nucleic acid; a detector probe to detect captured nucleic acid; a kit for molecular typing of biological sample using surface-enhanced Raman scattering (SERS) including resonance scattering (SERRS); and lastly a method of manufacturing said kit.

The invention relates to a method for determining chitosan content by a resonance Rayleigh scattering method sensitized by sodium dodecyl sulfate, and belongs to the field of macromolecule detection.Under certain conditions, chitosan can be combined with red amaranth to form an ionic associate to remarkably enhance resonance scattering intensity, and the sodium dodecyl sulfate is added as a sensitizer to improve the sensitivity. In a certain concentration range, the resonance Rayleigh scattering intensity and the chitosan content keep a linear relation in the certain concentration range, andthe resonance Rayleigh scattering intensity and the chitosan content keeping the linear relation are taken as a quantitative basis of the chitosan for establishment of the resonance Rayleigh scattering method of the chitosan. The method for determining the chitosan content is influenced by the molecular weight and the degree of deacetylation of the chitosan, and has the advantages of low cost, high sensitivity, good reproducibility and simple operation, and the like.

The present invention discloses a method for measuring the sulfite in the food with a chlorauric acid resonance scattering spectrometric determination. A 5mL color comparison tube is sequentially added with a 223.2 mu g / mL (calculated with Au(III)) HAuCl4 for 0.10-1.50mL, 1.0% aminosulfonic solution for 0.05-0.5mL and 116.0 mu g / mL sodium sulfite for 0.05-1.50mL, shaking up, adding the secondary distilled water to volume, swaying to uniform, and placing for 5-180min. On a fluorospectrophotometer, a synchronous scanning in which the inspiring wavelength is equal to the transmission wavelength is adopted, and the resonance scattering spectrometric of the system is obtained. The intensity I583nm of scattering light and reagent vacancy I0 are measured at 583nm. The value of delta I583nm is calculated with the equation: delta I583nm=I583nm-I0. Additionally the white sugar with right amount is added and is dissolved in water. The value of intensity delta I583nm is measured according to the method, and then the content of sulfite in the white sugar can be calculated out. The measuring method according to the invention has the advantages of high sensitivity, easiness and shortcut.