Surface plasma resonance absorption spectrometry method for determining NH<4+> by using sodium tetraphenylborate ligand for adjusting and controlling nano-silver catalytic activity

Abstract

The invention discloses a surface plasma resonance absorption spectrometry method for determining NH<4+> by using a sodium tetraphenylborate ligand for adjusting and controlling nano-silver catalytic activity. The method is characterized by comprising the following steps: (1) preparing a NH<4+> standard solution system with known concentration; (2) preparing a blank control solution system; (3) calculating according to the equation that delta A is equal to A minus A0; (4) making a working curve according to a relationship between the delta A and NH<4+> concentration; (5) preparing a to-be-measured sample solution, determining the resonance scattering peak intensity value of the to-be-measured sample solution as an A sample, and calculating according to the equation that delta A sample is equal to A sample minus A0; (6) calculating out the NH<4+> content of the sample solution according to the working curve. The method of the invention uses the ligand for adjusting and controlling nano-enzyme catalytic activity, and is simple, convenient, quick and high in sensitivity.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for measuring NH by regulating the catalytic activity of nano-silver with a surface plasmon resonance absorption spectrum using a sodium tetraphenylborate ligand. 4 + Methods. Background technique [0002] Nanozyme is an artificial enzyme, also known as nanomimetic enzyme, which was first proposed by Scrimin and Pasquato and used to describe gold clusters with significant ribonuclease activity. Due to the rapid development of the field of nano research and the unique physicochemical properties of nanomaterials, the impact of nanozymes has been popularized in countless fields, including: biosensing, immunoassays, cancer diagnosis and treatment, neuroprotection, stem cell growth and pollutants Clear etc. CeO 2 There are two mixed valence states of trivalent and tetravalent cerium and oxygen vacancies in it, so it shows excellent catalytic performance in various appl...

AI Technical Summary

Problems solved by technology

The titration method is based on the reaction of formaldehyde and ammonium to generate hexamethylenetetramine and hydrogen ions, and then titrate with NaOH. At the same time, titration of hydrogen ions is required to deduct the NaOH consumed by hydrogen ions. The method is cumbersome and cannot be used for trace amounts of ammonium. Ion chromatography has the advantages of good reproducibility and high accuracy, but it is time-consuming and susceptible to interference from other coexisting ions; fluorescence analysis has the characteristics of easy operation, sensitivity, and rapidity, but there are many interference factors and the analysis process Longer; high-quality chromatography, but the separation effect is unstable, etc.

So far, there has been no report on the determination of ammonium ions by surface plasmon resonance absorption spectroscopy catalyzed by tetraphenylborate sodium ligands regulated by nanozymes

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