Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Amplification method of pancreatic cells and differentiation method and application of pancreatic precursor-like cells

A technology of pancreatic cells and islet-like cells, which can be applied in the biological field and can solve the problems of long cycle, complicated procedures, and carcinogenic risk.

Pending Publication Date: 2021-07-23
SHANGHAI CELLIVER BIOTECHNOLOGY CO LTD +1
View PDF1 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, cells with insulin secretion function can be obtained by gradually inducing embryonic stem cells, but the induction process is complicated, long-term and costly; in addition, in the cell mass induced by embryonic stem cells, cells with insulin secretion function The proportion generally does not exceed 30%, and the later purification process is easy to contaminate the cell mass, so that the obtained cells with insulin secretion function have a potential carcinogenic risk

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Amplification method of pancreatic cells and differentiation method and application of pancreatic precursor-like cells
  • Amplification method of pancreatic cells and differentiation method and application of pancreatic precursor-like cells
  • Amplification method of pancreatic cells and differentiation method and application of pancreatic precursor-like cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038]In order to make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention are clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, and Not all examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention. Unless otherwise defined, the technical terms or scientific terms used herein shall have the usual meanings understood by those skilled in the art to which the present invention belongs. As used herein, "comprising" and similar words mean that the elements or items appearing before the word include the elements or items listed after the word and their equivalents, without excluding ot...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an amplification method of pancreatic cells. The amplification method comprises the following steps: sequentially carrying out digestion treatment, resuspension treatment, discontinuous density gradient centrifugation treatment and amplification treatment on pancreatic tissues. According to the amplification method disclosed by the invention, pancreatic ducts of mammals are used as sources of pancreatic precursor-like cells, and islet cells and acinar cells in obtained cell clusters are removed after the discontinuous density gradient centrifugal treatment, thus being good for improving the yield of pancreatic precursor-like cells, and avoiding ethical limitations and possible carcinogenic risk caused by the use of embryonic stem cells; and an amplification culture medium used in the invention comprises a reprogramming substance consisting of a plurality of small molecule compounds, so as to avoid the risks of nonspecificity, off-target deletion, etc., which are easily caused by changing gene sequences by using a gene editing method. The invention also provides a differentiation method and application of the pancreatic precursor-like cells obtained by the amplification method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to methods and applications for the expansion and differentiation of pancreatic cells. Background technique [0002] Diabetes mellitus is a chronic metabolic disorder characterized by insufficient insulin secretion or hyperglycemia caused by insulin resistance in surrounding tissues. In the advanced stage, it can lead to damage and dysfunction of tissues and organs throughout the body, especially the eyes, kidneys, cardiovascular system and nervous system. organ failure. Drug therapy and insulin supplementation are currently the most common treatments for diabetes, but they cannot completely cure diabetes, and it is difficult to prevent the development of long-term complications. [0003] Human β-cells can secrete insulin, reduce blood sugar, and regulate fat and protein metabolism. Human embryonic stem cells, because of their unlimited proliferation ability, can theoretically generate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C07K14/62C12P21/02
CPCC12N5/0676C07K14/62C12P21/02C12N2501/10C12N2501/91C12N2501/335C12N2501/999C12N5/0678C12N2501/415C12N2501/727C12N2501/15
Inventor 张洪丹
Owner SHANGHAI CELLIVER BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products