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Application of formononetin in preparation of medicine for preventing and treating acute pancreatitis diseases

A technology of formononetin and acute pancreatitis, which is applied in the application field of medicine, can solve the problems of acute pancreatitis pathogenesis not fully clarified, pancreatic organ necrosis, etc., so as to relieve acinar cell damage and inflammatory infiltration, reduce Content, the effect of broadening the application field

Pending Publication Date: 2021-10-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the survey, about 20% of AP patients will develop moderate or severe acute pancreatitis, with necrosis of the pancreas and surrounding tissues or organs, or both, and the mortality rate is as high as 20-40%. However, the pathogenesis of acute pancreatitis is not yet fully understood, so no radical treatment exists

Method used

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  • Application of formononetin in preparation of medicine for preventing and treating acute pancreatitis diseases
  • Application of formononetin in preparation of medicine for preventing and treating acute pancreatitis diseases
  • Application of formononetin in preparation of medicine for preventing and treating acute pancreatitis diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The establishment of embodiment 1 acute pancreatitis model

[0034] 8-week-old 20-23g female C57BL / 6J mice were randomly divided into control group (CON), inflammation model group (CAE), and formononetin pretreatment group (CAE+FOR), 8 in each group, Provide controlled rearing conditions. The acute pancreatitis model was induced by intraperitoneal injection of cerulein. Dissolve cerulein in normal saline, inject cerulein (50 mg / kg) into mice intraperitoneally, once every 1 hour, and inject 10 times in total, mice in CON group are given equal volume of normal saline. The formononetin pretreatment group was administered intragastrically with formononetin dissolved in 1% DMSO every day before modeling, the dose was 100mg / kg, and the duration of intragastric administration was 7 days. Equivalent 1% DMSO. Three hours after the last injection, the mice were injected with a lethal dose of pentobarbital sodium, and blood, pancreas and other samples were quickly collected.

Embodiment 2

[0035] Example 2 Formononetin can alleviate pancreatic edema, infiltration of neutrophils and reduce the increase of serum amylase and lipase in mice with acute pancreatitis

[0036] Pancreatic edema is one of the most intuitive indicators that can reflect the severity of acute pancreatitis. The main cause of edema is the recruitment and infiltration of a large number of immune cells in the pancreas when acute pancreatitis occurs. Among these abundant infiltrating immune cells, the activation of neutrophils plays an important role in the pathological process of acute pancreatitis. Myeloperoxidase is an important enzyme released by neutrophils, and its activity level can indirectly reflect the activity and infiltration of neutrophils. Cut part of the fresh pancreatic tissue, after peeling off the fat, use filter paper to gently absorb the water on the surface of the pancreatic tissue, place it on a pre-weighed tinfoil paper, and use an analytical balance to weigh the wet weigh...

Embodiment 3

[0038] Example 3 Formononetin can relieve acinar cell damage and inflammatory infiltration in mice with acute pancreatitis

[0039] Using H&E staining method, the pancreas of CON group, CAE group and CAE+FOR group were fixed, dehydrated, stained, dewaxed, cleared and mounted, and the integrity of pancreatic tissue, degree of pancreatic edema and inflammatory cells were observed. degree of infiltration. Such as figure 2, the results showed that the pancreatic tissue structure of the CON group was compact without obvious cracks. In the pancreas group of CAE mice, vacuoles and death of acinar cells, inflammatory cell infiltration, duct distortion and obstruction were observed, and pancreatic tissue damage was obvious. Compared with the CAE group, the CAE+FOR group has a significant improvement effect, indicating that formononetin has a protective effect on acute pancreatitis in mice.

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Abstract

The invention discloses application of formononetin in preparation of a medicine for preventing and treating acute pancreatitis diseases, and belongs to the field of biological medicines. According to the invention, by utilizing the formononetin, pancreatic edema is relieved, the content of serum amylase and serum lipase is reduced, acinus cell injury and inflammation infiltration are relieved, colon goblet cell injury and inflammation infiltration caused by acute pancreatitis are relieved and release of proinflammatory cytokines in pancreas and colon is reduced. According to the invention, the formononetin is used for preparing the medicine and the medicine composition for treating the acute pancreatitis and the related diseases of the acute pancreatitis, and the application field of the formononetin is widened.

Description

technical field [0001] The invention belongs to the field of biomedicine, and particularly relates to the application of formononetin in the preparation of medicines for preventing and treating acute pancreatitis diseases. Background technique [0002] Acute pancreatitis is an autoimmune disease with a continuously increasing incidence rate. It has an acute onset and rapid progression. It is an acute inflammation caused by the digestion of the pancreas and its surrounding tissues by trypsin. The main manifestations are edema, hemorrhage and necrosis of the pancreas. . The main causes of the disease are biliary system diseases, alcoholism and overeating, surgery and injury, infection, hyperlipidemia, etc. AP often involves the peripancreatic tissue, triggering an inflammatory cascade reaction and causing systemic inflammatory response syndrome (SIRS) ) and multiple organ dysfunction syndrome (MODS). According to the survey, about 20% of AP patients will develop moderate or ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P1/18A61P29/00
CPCA61K31/352A61P1/18A61P29/00
Inventor 潘礼龙杨军孙嘉吴迪顾元龙
Owner JIANGNAN UNIV
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