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Production method and application of high-yield gamma-aminobutyric acid

An aminobutyric acid, high-yield technology, applied in the fields of medical biology and food engineering, can solve the problems of chemical synthesis method with many by-products, unsuitable for industrial production, low GABA content, etc., and achieves improvement of brain function, good social and economic benefits, The effect of improving liver function

Active Publication Date: 2012-07-11
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, there are many by-products in the chemical synthesis method, which are difficult to purify, high in production cost, and poor in safety.
Although the process of plant-synthesized GABA is simple, the content of GABA enriched by plants is too small to be easy to extract, so it is not suitable for industrial production
Escherichia coli is generally used as the strain for biosynthesis, but there are hidden dangers in safety and health

Method used

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  • Production method and application of high-yield gamma-aminobutyric acid
  • Production method and application of high-yield gamma-aminobutyric acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Obtaining of Enterococcus raffinose strain M1 (CGMCC No.5584)

[0046] (1) Preliminary screening of the strains: from pickled cabbage soups naturally pickled in 12 regions in the three provinces of Northeast my country, using double-layer plate method, that is, containing 1% calcium carbonate and sodium glutamate, cultured at 30°C for 48 hours, and selected at high temperature. The colony with a transparent circle grown on a sodium glutamate plate with a high concentration was fermented statically. The fermentation temperature was 30°C and the fermentation time was 3 days. The content of GABA was detected by high-performance liquid chromatography, and a cotton seed with a yield of 16.95g / L was screened out. Enterococcus saccharomyces, numbered M3, was transferred on a slant and stored in a refrigerator at 4°C;

[0047] (2) Re-screening of strains: double-layer plate method is also used for re-screening, and M3 is picked from two rings from the slope to the seed medium, wh...

Embodiment 2

[0053] Production of GABA by Enterococcus raffinose strain M1 (CGMCC No.5584)

[0054] will activate the Entercoccus raffinosus CGMCC No.5584 strain was inoculated in the seed medium and the activation steps were as follows: pick two rings of M1 from the slope into the seed medium, wherein the seed medium (g / L): 10 sucrose, 10 peptone, yeast extract powder 5. Ammonium citrate 2, magnesium sulfate 0.58, manganese sulfate 0.25, sodium acetate 2, potassium dihydrogen phosphate 2;, culture at 30°C for 24 hours, and transfer to the fermentation medium when the amount of bacteria reaches the fermentation conditions.

[0055] Seed medium (g / L) and cultivation method: 10 sucrose, 10 peptone, 5 yeast extract powder, 2 ammonium citrate, 0.58 magnesium sulfate, 0.25 manganese sulfate, 2 sodium acetate, and 2 potassium dihydrogen phosphate. Seed culture conditions: 30 ° C incubator, static culture for 12 hours. According to the inoculation amount of 10-15%, it is inserted into a 10L fe...

Embodiment 3

[0058] Production of GABA by Enterococcus raffinose strain M1 (CGMCC No.5584)

[0059] will activate the Entercoccus raffinosus The CGMCC No.5584 strain was inoculated in the seed medium for cultivation, and was transferred to the fermentation medium when the bacterial mass reached the fermentation conditions.

[0060] Seed medium (g / L) and cultivation method: 10 sucrose, 10 peptone, 5 yeast extract powder, 2 ammonium citrate, 0.58 magnesium sulfate, 0.25 manganese sulfate, 2 sodium acetate, and 2 potassium dihydrogen phosphate. Seed culture conditions: 30 ° C incubator, static culture for 12 hours. According to the inoculation amount of 10-15%, it is inserted into a 10L fermenter.

[0061] Fermentation medium (g / L) and culture method: sucrose 20, yeast extract powder 15, sodium acetate 2, potassium phosphate 2, aluminum sulfate 0.3, magnesium sulfate 0.4, ammonium sulfate 0.2, Tween 80 0.2mL. The culture temperature of the fermenter was controlled at 30°C and the initial ...

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Abstract

The invention discloses a production method and an application of high-yield gamma-aminobutyric acid. The conservation number of high-yield gamma-aminobutyric acid (GABA) raffinose enterococcus M1 is CGMCC (China General Microbiological Culture Collection Center) No.5584. The production method comprises the following steps of: separating and selecting strains to obtain an Entercoccusraffinosus TCCC11660 (CGMCCNo. 5584) strain of high-yield gamma-aminobutyric acid; optimizing a fermentation culture medium and fermentation conditions; and carrying out a 10L fermentation tank experiment. The production method has the advantages of cheap raw materials, low production energy consumption, low production cost, good product safety and the like, and industrial production is easy to realize. The strain is applied to production of gamma-aminobutyric acid through glutamic acid fermentation and has good social and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of medical biology and food engineering, is an organic combination of modern biological and chemical engineering and traditional fermentation engineering technology, and improves the current production method of gamma-aminobutyric acid (GABA) by biotechnology. More specifically, Enterococcus raffinosus ( Entercoccus raffinosus ) CGMCC No.5584 strain, through the biotransformation of growing cells, can efficiently produce γ-aminobutyric acid. Background technique [0002] γ-aminobutyric acid (γ-aminobutyric acid, GABA) is a kind of natural amino acid composed of non-protein, which widely exists in animals and plants. As an important inhibitory neurotransmitter in the central nervous system, GABA also plays an important role in the energy metabolism of the human brain. It participates in a variety of metabolic activities, and has the functions of lowering blood pressure, regulating arrhythmia, improving slee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12P13/00C12R1/01
Inventor 高强高年发朱燕陈慧敏段强王梦晓
Owner TIANJIN UNIV OF SCI & TECH
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