The invention relates to a bactericidal composition containing mepanipyrim. The bactericidal composition is prepared from an active component A and an active component B, wherein the active component A is selected from mepanipyrim, the active component B is selected from one of thiophanate methyl, polyoxins, carbendazim and prochloraz, and the weight ratio of the active component A to the active component B is 1:80-80:1. The effective active components are added into corresponding auxiliaries to be prepared into wettable powder, water dispersible granules, a suspending agent, suspension emulsion, emulsion in water, microemulsion, microcapsule suspension and a microcapsule suspension-suspension agent; the bactericidal composition is used for preventing and controlling gray mold, powdery mildew, venturia inaequalis, downy mildew, a rice blast, a rust disease, anthracnose, a leaf spot disease, an epidemic disease, a brown spot, an alternaria leaf spot and damping-off, resists rainwater washing and has the significant synergistic effect.