The invention discloses a fungicidal composition containing fluoxastrobin and tebuconazole and application of the composition. According to the fungicidal composition, fluoxastrobin and tebuconazole serve as main effective components, and the mass ratio of fluoxastrobin to tebuconazole is 70:1-1:50. The fungicidal composition can be applied to preventing diseases of cereal, fruit trees and vegetables, has a high synergistic function, overcomes and delays the insecticide resistance of germs, is high in fungicidal speed and long in lasting time, reduces the application cost, and has the prevention effect which is obviously better than that of single fungicides in use. The fungicidal composition can be used for preventing fungal diseases on crops, can be particularly used for preventing head blight, the rust disease, powdery mildew, net blotch, root rot, smut, downy mildew, rice sheath blight and other fungal diseases, and has effects better than those of the single fungicides in use.