The invention discloses a sterilization composition containing js399-19. The sterilization composition comprises strobilurin bactericide of which the first active component is js399-19 and the second active component is selected from kresoxim-methyl, pyraclostrobin, trifloxystrobin, orysastrobin, fluoxastrobin, picoxystrobin and metominostrobin, wherein the weight ratio of the first active component to the second active component is 1:10-15:1, preferably 1:5-8:1. The composition can be used for preparing agriculturally permitted water dispersible granules, wettable powder, suspending agent, flowable concentrate for seed coating, seed treatment dispersible powder and seed treatment dispersible granule preparation. The sterilization composition has the advantages of reasonable components, good sterilization effect and low application cost, and the activity and sterilization effects of the composition are not simple superposition of the component activities. Compared with theconventional single preparation, the composition has remarkable sterilization effect, remarkable synergic effect, no pollution to crops and good safety, meets the safety requirement of pesticide preparation, and has remarkable prevention effects on wheat take-all, gibberellic disease, rust disease, pyricularia oryzae and bakanae disease.