50results about How to "Use less blood" patented technology

The invention provides a microfluidic chip and a circulating tumor cell capture method using the same. The microfluidic chip comprises a separating cavity, a micro-pillar array and an external magnetic field assembly, wherein a sample injection opening is formed in the middle of a first side of the separating cavity; a to-be-detected particle enrichment part and a sample outflow opening are respectively arranged at the upper part and the lower part of a second side; the micro-pillar array is arranged in the separating cavity and between the sample injection opening and the sample outflow opening; the external magnetic field assembly is used for forming a magnetic field inside the separating cavity from the bottom up; among samples injected from the sample injection opening, to-be-detected particles of which the surfaces are connected with magnetic beads move upwards under the application force of the magnetic field, and are enriched at the to-be-detected particle enrichment part or attached onto the top wall of the separating cavity, and to-be-detected particles of which the surfaces are not connected with magnetic beads are intercepted and captured by the micro-pillar array; besides the to-be-detected particles, other components in the samples penetrate through the micro-pillar array under the gravity action. According to the method, two capture modes are combined, so that high capture rate and high throughput are realized, and high-purity circulating tumor cells are captured.

The invention belongs to the technology field of the cellular immunology, and particularly relates to a method for the dominant amplification of in vitro peripheral blood nature killer cells with a large amount. The method comprises the steps as follows: (1) separating single nucleus cell from human or animal peripheral blood; (2) suspending the single nucleus cell in an RPMI 1640 culture solution containing 5%-15% autologous serum and pretreating with methyl-Beta-cyclodextrin with an effective concentration of 1-4mmol / L for 36-60 hours; and (3) adding recombined interleukin 2, and amplification-culturing in an RPMI 1640 culture solution containing 5% of new-born calf serum and 5% of autologous serum for above 10 days. The method has the advantages that the blood sample amount is small; the cost is low; the operation is convenient; and the amplification multiple is high, and the nature killer cells can be amplified by 392-1752 times in a short term.

The invention relates to a micro-fluidic chip used for testing erythrocyte osmotic fragility, which comprises a chip main-body, wherein, two solution inlets and a blood sample inlet are formed on the chip main-body; and a plurality of detection pools arranged inside the chip main-body are communicated with the blood sample inlet through a blood sample microchannel and communicated with the two solution inlets through a microchannel network that generates concentration gradient automatically. Based on the micro-fluidic chip, a test method of erythrocyte osmotic fragility comprises the steps asfollows: (1) blood samples are introduced into the different detection pools in the micro-fluidic chip through the blood sample inlet; (2) two NaCl solutions with different concentrations are introduced into the micro-fluidic chip through the two solution inlets at the same flow speed at the same time, and mixed with the blood samples in the detection pools; (3) erythrocytes in the detection pools are observed through a microscope and are shoot; and (4) the intact erythrocytes in each detection pool are distinguished in erythrocyte pictures and counted. The micro-fluidic chip reduces use of blood, reduces manual intervention, and has high detection speed and objective and accurate detection results.

The invention relates to a set of a kit for detecting specificity platelet antoantibody by the combination of monoclonal antibody and nano-microspheres. The kit comprises each 3 ml of four types of monoclonal antibody-polystyrene nano-microspheres PBS solution, 6 ml goat-anti-human polyclonal antibody and PBS solution, wherein the four types of the monoclonal antibody-polystyrene nano-microspheres PBS solution are sealed by bovine serum albumin and are respectively connected with a monoclonal antibody SZ-2, a monoclonal antibody SZ-22, a monoclonal antibody SZ-21, a monoclonal antibody 7E3, and the concentration of polystyrene nano-microspheres is 2 mg / ml; the goat-anti-human polyclonal antibody is connected with fluorescein isothiocyanate and with the concentration of the goat-anti-human polyclonal antibody is 15 mug / ml. A detection process of the specificity platelet antoantibody comprises the following steps of: oscillating monoclonal antibody--microspheres and platelet lysis buffer sample for incubation; washing by PBS; adding the goat-anti-human polyclonal antibody connected with the fluorescein isothiocyanate; washing, re-suspending by the PBS; detecting on a flow cytometry to obtain average fluorescence intensity values of the four types of the nano-microspheres respectively; and confirming whether the specificity platelet antoantibody is positive or not according to a ratio of the obtained values to a basic contrast value of that of a healthy person, in order to provide foundation for early diagnosis of ITP. The detection has the advantages of simple process, high sensitivity and good specificity.

The invention discloses a kit for detecting TORCH IgM antibodies. The kit comprises a solid carrier which is coated with an antibody combined with the TORCH IgM antibodies, a TORCH antigen which is marked with lanthanide, a TORCH IgM antibody calibrator, a sample diluent, an experiment buffering solution, a concentration cleaning solution and an enhancement solution. The kit can simultaneously detect multiple pathogene IgM antibodies in TORCH pathogene under the same detection condition and is high in sensitivity, high in specificity, good in precision, high in accuracy, little in blood consumption amount, rapid, convenient and favorable for early diagnosis on the infection of the TORCH pathogene. The method has the characteristic that the detection linear range is wide, the diluting times or diluting ratio of a high-value sample can be reduced, and the accuracy of a detection result can be improved.

The invention relates to a newborn baby TRECs and KRECs gene copy number detection kit using a digital PCR technology. The kit comprises a forward primer, a reverse primer and a probe used for detecting a TRECs gene, a forward primer, a reverse primer and a probe used for detecting a KRECs gene, and a forward primer, a reverse primer and a probe used for detecting a reference gene TRAC. The detection of the project can be completed only through hole forming to drill a round dried blood spot (with the blood quantity about 3mul) with the diameter being 3mm; the blood consumption is low; the kitis very suitable for the newborn baby screening unsuitable for great blood sampling quantity. Two rounds of sequential detection strategy are used; in the first round of detection, only the synchronous detection on the TRECs and KRECs gene copy number by single hole is needed; the two results are quality control reference indexes for each other. The second round of reference gene TRAC copy numberdetection is needed only when the TRECs and KRECs gene copy numbers are lower than the threshold values at the same time; whether the detection control loss due to sample quality problem occurs or notis determined.

The invention belongs to the technical field of medical instruments, and particularly relates to a disposable hard connecting band collection container and an anti-freezing vacuum blood taking needle of a bleeding opening. The disposable hard connecting band collection container is formed in the following mode that: an anticoagulant coating layer is arranged on an inner cavity wall of a circular connector; one end of the connector is provided with a venous blood taking needle; the rear part of the connector is provided with a vacuum valve; the other end of the connector is provided with the blood collection sample bottle needle; the blood collection sample bottle needle is connected with a blood collection sample bottle cap; the blood collection sample bottle cap is connected with a rubber plug; the rubber plug is connected with a blood collection sample bottle; and one side on the blood collection sample bottle close to the blood collection sample bottle cap is provided with one secondary bleeding opening. The disposable hard connecting band collection container and the anti-freezing vacuum blood taking needle of the bleeding opening have the advantages that: the anticoagulant coating layer is added on the basis of the conventional hard connection type blood taking needle so that blood flowing out of a body can contact an anticoagulant; a blood sample is ensured to generate no cruor and hemolysis so as to ensure the accuracy of the test result; and the secondary bleeding opening is added so that a clinical inspector can flexibly control the blood dosage during the test to ensure repeated tests at any time or add new testing items.

The invention discloses a combined-type clinical analyzer comprising the following components sequentially connected through rails: a sample stage used for placing a sample rack, an electrolyte analyzer used for carrying out electrolyte analysis upon the sample in the sample rack, a biochemical analyzer used for carrying out biochemical analysis upon the sample in the sample rack, and a chemiluminescence immunoassay analyzer used for carrying out chemiluminescence immunoassay upon the sample in the sample rack. The sample stage is connected with the electrolyte analyzer through an electrolyte rail. The electrolyte analyzer is connected with the biochemical analyzer through a biochemical rail. The biochemical analyzer is connected with the chemiluminescence immunoassay analyzer through a luminescence rail. According to the invention, the electrolyte analyzer, the biochemical analyzer and the chemiluminescence immunoassay analyzer are connected through rails as independent modules, such that automatic operation of detection is realized. Therefore, large-batch sample detection can be performed, and blood consumption during detection is greatly reduced.

The invention belongs to the field of molecular cytogenetics and relates to an amplified composition for fast gene detection of microdeletion of Y chromosomes, a kit containing the same and an application thereof. The amplified composition comprises a group A and a group B, wherein each group comprises a pair of primers respectively in AZFa, AZFb and AZFc areas and two pairs of internal control primers ZFY and SRY; the primers in the group A have the nucleotide sequence shown in SEQ ID No.1-1O; the primers in the group B have the nucleotide sequence shown in SEQ ID No.11-2O. The amplified composition, the kit and the application have the advantages that on the basis of the prior art, multiple PCR (Polymerase Chain Reaction) buffering liquid and Tag polymerase are improved, and trace whole-blood amplification is directly adopted due to less blood consumption, so that the pain of a patient is reduced; and the procedure of extracting DNA is omitted, so that the time and the labor are saved and the economic expenditure is greatly reduced.

The invention discloses a novel cryoprecipitated antihemophilic factor preparing instrument. The novel cryoprecipitated antihemophilic factor preparing instrument is characterized by comprising an upper blood bag box and a lower blood bag box which are connected through a pipeline, a height difference exists between an upper blood bag and a lower blood bag, the upper blood bag and the lower blood bag are both connected with weighing mechanisms, and a melted water tank is arranged inside the upper blood bag and connected with a water circulating device. The automation degree is high, the influence of human factors is reduced, cryoprecipitation quality is improved correspondingly, the cryoprecipitation curative effect is improved, medical burden is relieved for patients, blood consumption of a blood station is reduced, and workers do not make contact with ice water any more during blood bag taking and placing so that the working environment is improved.

The invention discloses a reagent for detecting anti-platelet surface receptor-specific autoantibodies and a preparation method and application thereof. The reagent has high specificity and sensitivity. The method comprises expressing a human platelet-specific receptor membrane glycoprotein GP Ib-IX and / or GP IIb / IIIa receptor through CHO cells, carrying out co-incubation on the specific membraneglycoprotein receptor expression-positive CHO cells and serum to be detected and adding a fluorescein-labeled anti-human immunoglobulin polyclonal antibody (secondary antibody) for bonding. If there is an anti-platelet receptor-specific antibody in serum or plasma, the CHO-membrane glycoprotein specific antibody-fluorescein-labeled anti-human immunoglobulin polyclonal antibody complex structure isformed, has high CHO cell fluorescence intensity and can be used for flow cytometry or fluorescence microscope examination. The method is easy to operate, has enough specific membrane glycoprotein expression positive CHO cell sources, high specificity and sensitivity and high diagnosis efficiency and can be used for basic research and clinical examination.

The invention relates to a measuring instrument for detecting the viscosity of human blood in real time. The measuring instrument includes a pressure source, an infusion tube, a micrometer tube, a transparent capillary, a calibration liquid container containing calibration liquid, a temperature control unit, a data measurement and processing unit, a display module, and a control unit; the transparent capillary is inside the data measurement and processing unit. , and the viscosity was measured using the relative measurement method. The measuring instrument for real-time detection of human blood viscosity of the invention can eliminate operation and reading errors, and has high detection accuracy and automation; the blood volume is small, and only about 20ul of blood can be used for measurement; Sexual use to avoid infection; simple operation, easy to carry, suitable for clinical use in hospitals or ordinary households to detect human blood viscosity.

The invention relates to a real-time fluorescent quantitative PCR kit for detecting TRECs and KRECs genes. The kit comprises: 1) a DNA extraction reagent; 2) a standard product containing a TRECs geneinsertion sequence, a KRECs gene insertion sequence and a TRAC gene insertion sequence; 3) a fluorescent PCR reaction solution I containing fluorescent PCR primers and detection probes of the TRECs gene and the TRAC gene; and 4) a fluorescent PCR reaction solution II containing fluorescent PCR primers and detection probes of the KRECs gene and the TRAC gene. The kit has the advantages of high sensitivity, good specificity, simple and rapid detection method, reliable experimental result, realization of screening of immunodeficiency diseases mainly including SCID and antibody deficiency, provision of clinically relevant indications for other primitive immunodeficiency diseases related to T cell and B cell development or other systemic diseases, and facilitation of early diagnosis and treatment.

The invention relates to a reagent and a test cup used for rapid detection of blood viscoelasticity. The reagent comprises solvent water, 2 to 8g / L of a buffering agent, 0.1 to 1g / L of NaOH, 0.1 to 0.5g / L of propyl gallate, and 10 to 30g / L of CaCl2, and the pH value ranges from 6.0 to 9.0. According to the reagent, propyl gallate is taken as an activator, is capable of activating platelet membranephosphatidylserine (PF3, blood clotting catalytic surface) internal and external membrane turnover, activating an endogenous blood clotting system at the same, and starting blood clotting process inthe presence of calcium ions. After treatment, propyl gallate is capable of dissolving in water, so that uniform mixing in reaction can be ensured, and propyl gallate is not influenced by aprotinins .

The invention relates to a real-time fluorescent quantitative PCR (polymerase chain reaction) kit for one-step quantitative detection of KRECs gene and its application. The kit comprises a real-time fluorescent quantitative PCR reaction system based on real-time fluorescent PCR technique. The fluorescent PCR reaction system comprises forward and reverse primers specific to KRECs and beta-actin genes, and a specific fluorescent probe. The kit allows quick screening of neonatal immune system B-cell level, has high sensitivity and stability and provides excellent reproducibility, and this method is applicable to the quantitative detection of KRECs and to the functional screening of the neonatal immune system and is worthy of practical clinical application.

The invention discloses a kit for detecting hepatocellular carcinoma (HCC) and belongs to the technical field of medical examination. The kit comprises five pairs of primers for detecting five methylation specific sites of a genome, and the sequences of the primers are represented by SEQ ID No.1-10. Genome DNA of the blood of a person to be detected is extracted; after bisulfate processing, 5 pairs of primers are subjected to PCR amplification, the amplification products are connected to a clone carrier, cloning and sequencing are performed; according to the sequencing results, the number of methylation sites is determined; then the ctDNA of the blood of the person is extracted, and the length of the ctDNA is measured. If the number of methylation sites is 3 or more, the person has HCC; and if the number of methylation sites is 1 or 2 and the length of ctDNA is less than 245 bp, the person has HCC. The kit has the advantages that the amount of used blood is little, the operation is convenient, the cost is low, and the accuracy is high; and can be applied to early diagnosis and mass screening of HCC.

The invention relates to a micro-fluidic chip used for testing erythrocyte osmotic fragility, which comprises a chip main-body, wherein, two solution inlets and a blood sample inlet are formed on the chip main-body; and a plurality of detection pools arranged inside the chip main-body are communicated with the blood sample inlet through a blood sample microchannel and communicated with the two solution inlets through a microchannel network that generates concentration gradient automatically. Based on the micro-fluidic chip, a test method of erythrocyte osmotic fragility comprises the steps asfollows: (1) blood samples are introduced into the different detection pools in the micro-fluidic chip through the blood sample inlet; (2) two NaCl solutions with different concentrations are introduced into the micro-fluidic chip through the two solution inlets at the same flow speed at the same time, and mixed with the blood samples in the detection pools; (3) erythrocytes in the detection pools are observed through a microscope and are shoot; and (4) the intact erythrocytes in each detection pool are distinguished in erythrocyte pictures and counted. The micro-fluidic chip reduces use of blood, reduces manual intervention, and has high detection speed and objective and accurate detection results.

The invention discloses a device of extracorporally imitating blood pressure fluctuation rising and an application method and an application, and relates to the field of medical research tools. An imitation blood pressure pump and an imitation blood pressure fluctuation pump are respectively arranged, so that imitation blood pressure and blood pressure fluctuation change are suffered from the adjustment of different regulating units. Separating adjusting effects of the imitation blood pressure and blood pressure fluctuation change are achieved. The device of extracorporally imitating blood pressure fluctuation rising solves the problem that no proper extracorporal model for studying the blood pressure fluctuation rising exists.

The invention discloses a device of extracorporally imitating blood pressure fluctuation rising and an application method and an application, and relates to the field of medical research tools. An imitation blood pressure pump and an imitation blood pressure fluctuation pump are respectively arranged, so that imitation blood pressure and blood pressure fluctuation change are suffered from the adjustment of different regulating units. Separating adjusting effects of the imitation blood pressure and blood pressure fluctuation change are achieved. The device of extracorporally imitating blood pressure fluctuation rising solves the problem that no proper extracorporal model for studying the blood pressure fluctuation rising exists.

The invention relates to a real-time fluorescence quantitative PCR (polymerase chain reaction) kit for quantitatively detecting KRECs gene and application thereof. The kit comprises a PCR system based on nested PCR technology and a real-time fluorescence quantitative PCR system based on real-time fluorescence PCR technology, wherein the nested PCR system comprises forward and reverse primers for KRECs and beta-actin genes; and the real-time fluorescence quantitative PCR system comprises forward and reverse primers and specific fluorescence probes for KRECs and beta-actin genes. The kit can be used for quickly screening the B-cell level of a neonatal immune system, and has the advantages of high sensitivity, high stability and excellent reproducibility. The method is suitable for quantitative detection of KRECs, can be used for screening functions of the neonatal immune system, and has practical clinical application value.

The invention relates to a primer, detection kit and detection method for screening SCID genetic diseases. The kit comprises a DNA extract, a PCR MIX reaction solution and upstream and downstream primers and probes for TREC gene amplification, internal reference Calpha gene amplification and external control Calpha gene amplification; the sequences of the upstream and downstream primers for TREC gene amplification are shown as SEQ ID NO.1 and SEQ ID NO.2, and the sequence of the TREC gene probe is shown as SEQ ID NO.3; the sequences of the upstream and downstream primers for the internal reference Calpha gene amplification are shown as SEQ ID NO.4 and SEQ ID NO.5, and the sequence of the internal reference Calpha gene probe is shown as SEQ ID NO.6; the sequences of the upstream and downstream primers for the external control Calpha gene amplification are shown as SEQ ID NO.4 and SEQ ID NO.5, and the sequence of the external control Calpha gene probe is shown as SEQ ID NO.7. The methodis based on a fluorogenic quantitative PCR technology. A chelex100 method is used for dealing with dried blood spots for direct amplification to qualitatively detect T cell receptor deletion loops, and a rapid, efficient and sensitive SCID screening technology system is established. The detection method is convenient in operation, low in cost and easy to popularize.

The invention belongs to the technical field of medical instruments, and relates to a disposable soft connecting band collection container and an anti-freezing vacuum blood taking needle of a bleeding opening. The disposable soft connecting band collection container is formed in the following mode that: a biological product anticoagulant coating layer is arranged on an inner cavity wall of a circular connector; one end of the connector is provided with a blood taking plastic duct; a vacuum valve is placed on the plastic duct; the other end of the plastic duct is provided with the circular connector; the inner cavity wall of the circular connector is provided with the biological product anticoagulant coating layer; one end of the connector is provided with a venous blood taking needle, while the other end is provided with a blood collection sample bottle needle; the blood collection sample bottle needle is connected with a blood collection sample bottle cap; the blood collection samplebottle cap is connected with a rubber plug; the rubber plug is connected with a blood collection sample bottle; and one side on the blood collection sample bottle close to the blood collection samplebottle cap is provided with a secondary bleeding opening. The disposable soft connecting band collection container and the anti-freezing vacuum blood taking needle of the bleeding opening can ensure that a blood sample generates no cruor and hemolysis so as to ensure the accuracy of the test result. The blood dosage during the test can be controlled flexibly so as to ensure repeated tests at any time or the addition of new testing items.

The invention provides a device for connecting artificial blood vessels with aortas and innominate artery blood vessels in operations. The device comprises a connecting device body which is of a tubular structure, the inner side wall of the connecting device body is smooth, and a groove is formed in the outer side of the tubular structure along the circumference. According to the device, a traditional suturing technology can be replaced, the operation and deep hypothermic circulatory arrest time can be effectively shortened, and complications such as anastomosis bleeding are avoided, so that the blood consumption is greatly reduced, and the operation survival rate is increased.

The invention relates to a real-time fluorescence quantitative PCR (polymerase chain reaction) kit for quantitatively detecting TRECs gene and application thereof. The kit comprises a PCR system based on nested PCR technology and a real-time fluorescence quantitative PCR system based on real-time fluorescence PCR technology, wherein the nested PCR system comprises forward and reverse primers for TRECs and beta-actin genes; and the real-time fluorescence quantitative PCR system comprises forward and reverse primers and specific fluorescence probes for TRECs and beta-actin genes. The kit can be used for quickly screening the T-cell level of a neonatal immune system, and has the advantages of high sensitivity, high stability and excellent reproducibility. The method is suitable for quantitative detection of TRECs, can be used for screening functions of the neonatal immune system, and has practical clinical application value.

The invention discloses an electrochemical biosensor. The electrochemical biosensor comprises a substrate layer, an electrode output end is arranged at the upper end of the substrate layer, a reactionelectrode and an auxiliary electrode are arranged at the lower end of the substrate layer, a conductive circuit is connected between the reaction electrode, the auxiliary electrode and the electrodeoutput end, a hydrophilic film layer is arranged on a reaction channel at the lower end of the substrate layer, and a to-be-detected liquid inlet is formed in the front end of the hydrophilic film layer; air holes are respectively formed in the hydrophilic film layer at the rear end of the reaction channel; and reagent layers are arranged on the reaction electrode and the auxiliary electrode. Theinvention also discloses a method for measuring a blood impedance phase angle by using the electrochemical biosensor.

The invention provides a preparation method of an antibody chip for blood cell separation counting. The preparation method comprises the following steps of: S1, performing hydroxylation on a glass slide; S2, performing amination on the glass slide; S3, performing formylation on the glass slide; S4, coating a region with streptavidin; S5, incubating an antibody; S6, closing the coated region; S7, drying the glass slide; and S8, performing vacuum packaging. According to the preparation method of the invention, CD3, CD4 and CD8 cells fixed on the slide are distinguished from mononuclear cells through a peroxidase staining method, and automatic counting or manual counting is carried out.

The embodiment of the invention discloses a sample analyzer. The sample analyzer comprises a sample collection and distribution part, a reagent supply part, a test solution preparation part, a detection part, an analysis part and a controller, the embodiment of the invention further discloses a sample analysis method which is applied to the sample analyzer and is used for calculating and determining the target sample amount needing to be sucked according to the detection item or the detection mode input by the user, so that the blood sample sucked according to the constant amount is prevented from being wasted, and the blood use amount in the blood sample analysis process is reduced; the invention further discloses an animal blood sample analyzer applying the scheme.

The invention discloses a microscale serum insulin content detection device which includes a gas-control unit, a microfluidic integrated chip and a detection apparatus. The microfluidic integrated chip includes a glass base layer, a gas driving layer and a liquid flowing layer which are installed together in a successive manner from bottom to top. A mixer, a plurality of gas channel pipes, a plurality of structure gas chambers and a plurality of pneumatic micropumps are arranged on the gas driving layer and are communicated with each other. The gas channel pipes are communicated with the gas-control unit. A mixing pool, a plurality of fluid channels and a plurality of micro liquid storage pools are arranged on the liquid flowing layer, wherein the number of the fluid channels and the number of the micro liquid storage pools are corresponding to the number of the structure gas chambers. Each fluid channel is communicated with each micro liquid storage pool. The detection apparatus includes a photomultiplier. The device is small in size, little in blood consumption, low in cost, high in detection speed and high in sensitivity. The device has great significances of achieving popularized diabetes screening and early-stage diagnosis and medical treatment and reducing harms of large-scale epidemic diseases on human society.

The invention discloses a combined-type clinical analyzer comprising the following components sequentially connected through rails: a sample stage used for placing a sample rack, an electrolyte analyzer used for carrying out electrolyte analysis upon the sample in the sample rack, a biochemical analyzer used for carrying out biochemical analysis upon the sample in the sample rack, and a chemiluminescence immunoassay analyzer used for carrying out chemiluminescence immunoassay upon the sample in the sample rack. The sample stage is connected with the electrolyte analyzer through an electrolyte rail. The electrolyte analyzer is connected with the biochemical analyzer through a biochemical rail. The biochemical analyzer is connected with the chemiluminescence immunoassay analyzer through a luminescence rail. According to the invention, the electrolyte analyzer, the biochemical analyzer and the chemiluminescence immunoassay analyzer are connected through rails as independent modules, such that automatic operation of detection is realized. Therefore, large-batch sample detection can be performed, and blood consumption during detection is greatly reduced.

An automatic and synchronous replacement transfusion apparatus comprises: a transfusion apparatus (102) for injecting blood; a blood drawing apparatus (104) for drawing blood; and a main control apparatus (106), connected to the transfusion apparatus (102) and the blood drawing apparatus (104) separately, and for monitoring and / or controlling a dynamic balance between the amount of blood injected by the transfusion apparatus (102) and the amount of blood drawing by the blood drawing apparatus (104). For the automatic and synchronous replacement transfusion apparatus, the main control apparatus (106) is used to effectively monitor and / or control the blood injected by the transfusion apparatus (102) and the blood drawn by the blood drawing apparatus (104), thereby achieving a real-time dynamic balance in the transfusion and blood drawing process, and making the operation simple.