Microscale serum insulin content detection device

Abstract

The invention discloses a microscale serum insulin content detection device which includes a gas-control unit, a microfluidic integrated chip and a detection apparatus. The microfluidic integrated chip includes a glass base layer, a gas driving layer and a liquid flowing layer which are installed together in a successive manner from bottom to top. A mixer, a plurality of gas channel pipes, a plurality of structure gas chambers and a plurality of pneumatic micropumps are arranged on the gas driving layer and are communicated with each other. The gas channel pipes are communicated with the gas-control unit. A mixing pool, a plurality of fluid channels and a plurality of micro liquid storage pools are arranged on the liquid flowing layer, wherein the number of the fluid channels and the number of the micro liquid storage pools are corresponding to the number of the structure gas chambers. Each fluid channel is communicated with each micro liquid storage pool. The detection apparatus includes a photomultiplier. The device is small in size, little in blood consumption, low in cost, high in detection speed and high in sensitivity. The device has great significances of achieving popularized diabetes screening and early-stage diagnosis and medical treatment and reducing harms of large-scale epidemic diseases on human society.

Description

[0001] technical field [0002] The invention relates to the technical field of biological detection, in particular to a detection device for trace serum insulin content. Background technique [0003] With the development of society and economy and the change of lifestyle in various countries in the world, the number of diabetic patients in the world is increasing rapidly, and the threat of diabetes is becoming increasingly serious for mankind. Diabetes can cause a variety of chronic complications, which not only seriously affect the quality of life and life expectancy of patients, but also bring a heavy economic burden and become a killer that endangers human health. [0004] Blood sugar level is a common detection method for diagnosing diabetes at present, but it is only applicable to the sick period of diabetes. In recent years, there have been clinical cases of early diabetes cure, and early diagnosis is the premise of diabetes cure. Defective insulin action and impair...

AI Technical Summary

Problems solved by technology

[0005] At present, the methods used for insulin determination mainly include radioimmunoassay, enzyme-linked immunosorbent assay (ELISA) and chemiluminescence, etc., but there are common problems such as large instrument volume, large blood consumption, and high cost.

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