The invention discloses an eggshell membrane separation device, method and production line. The eggshell membrane separation device includes a shell, a drainage plate, an upper cover plate and a mixing motor. The drainage plate is provided with a diversion groove and a collecting groove, the diversion groove communicates with the collecting groove, the output end of the mixing motor is connected with a mixing paddle, and the mixing paddle is provided with a first large blade and a second small blade; and the shell is further internally provided with a plurality of arc baffles, the baffles aremovably attached to the inner wall of the shell, and each baffle is provided with one or more bulges. Under the combined action of mechanical agitation, shear breaking and physical water separation ofthe device, the cleaning of eggshell membranes and the high-efficiency continuous automatic separation discharging are realized simultaneously. By using the separation method, device and production line, the purity of the eggshell membranes obtained by separation can reach up to 97% or more, and moreover, by additionally arranging a recovery and purification water pool behind an egg membrane collection device, production water can be recycled. The separation method has the significant advantages of convenient operation, high separation efficiency, high purity of the separated egg membranes, continuous and automatic operation and cost saving.