243 results about "Laser-induced fluorescence" patented technology

The Laser Induced Fluorescence Attenuation Spectroscopy (LIFAS) method and apparatus preferably include a source adapted to emit radiation that is directed at a sample volume in a sample to produce return light from the sample, such return light including modulated return light resulting from modulation by the sample, a first sensor, displaced by a first distance from the sample volume for monitoring the return light and generating a first signal indicative of the intensity of return light, a second sensor, displaced by a second distance from the sample volume, for monitoring the return light and generating a second signal indicative of the intensity of return light, and a processor associated with the first sensor and the second sensor and adapted to process the first and second signals so as to determine the modulation of the sample. The methods and devices of the inventions are particularly well-suited for determining the wavelength-dependent attenuation of a sample and using the attenuation to restore the intrinsic laser induced fluorescence of the sample. In turn, the attenuation and intrinsic laser induced fluorescence can be used to determined a characteristic of interest, such as the ischemic or hypoxic condition of biological tissue.

Disclosed is a lateral flow quantitative assay method which can measure one or more analyte species at the same time, with high sensitivity. Also, the present invention relates to a strip which can measure one or more analyte species at the same time, with high sensitivity and a package in which the strip is integrated with a laser-induced surface fluorescence detector. The present invention can quantify multiple analytes with a minimum detection limit of pg / ml. Therefore, the present invention provides an advantage capable of quantifying a plurality of analytes at the same time using a simple lateral flow assay strip.

The invention is directed toward a micro-endoscope assembly for the treatment of diseased tissue in breast ducts comprising a cylindrical guide tube with a distal end defining an internal cylindrical passageway, a first smaller cylindrical tube eccentrically formed in the cylindrical passageway of a smaller diameter than said tube internal cylindrical passageway to receive and guide an endoscope, the smaller cylindrical tube forming together with an inner wall surface of the cylindrical guide tube a second passageway. A light transmitting probe is mounted in the second passageway and is connected at the distal end of the guide tube with an energy transmitting device. The light probe is activated to generate light at a particular wavelength to cause the tissue to fluoresce and is again activated to generate light at a specific wavelength to necrose the diseased tissue.

The invention discloses a method for detecting ochratoxin A by the magnetic separation of an aptamer-functionalized magnetic nano material and the marking of an up-conversion fluorescent nano material, which is used for detecting the ochratoxin A content of wheat, grains, feeds and products thereof, and the like. In the method, the ochratoxin-specific aptamer-functionalized magnetic nano material is utilized to perform magnetic separation and enrichment reaction on a sample to rapidly condense bulk samples, effectively increase the condensation of the samples by thousands of times, greatly shorten a detection period and improve the detection sensitivity; and simultaneously, the novel ochratoxin A analysis method for realizing rapid sample treatment, analysis and detection is constructed by combining the characteristics of high sensitivity of laser induced fluorescence of the up-conversion fluorescent nano material and capability of effectively avoiding the interference of biological background fluorescence of the samples.

Various embodiments of a multi-laser system are disclosed. In some embodiments, the multi-laser system includes a plurality of lasers, a plurality of laser beams, a beam positioning system, a thermally stable enclosure, and a temperature controller. The thermally stable enclosure is substantially made of a material with high thermal conductivity such as at least 5 W / (m K). The thermally stable enclosure can help maintain alignment of the laser beams to a target object over a range of ambient temperatures. Various embodiments of an optical system for directing light for optical measurements such laser-induced fluorescence and spectroscopic analysis are disclosed. In some embodiments, the optical system includes a thermally conductive housing and a thermoelectric controller, a plurality of optical fibers, and one or more optical elements to direct light emitted by the optical fibers to illuminate a flow cell. The housing is configured to attach to a flow cell.

Improved apparatus is disclosed for carrying out axially illuminated laser induced fluorescence whole-column imaging detection in the capillary isoelectric focusing of proteins. The separation capillary was made of low refractive index Teflon conditioned with methylcellulose to reduce electroosmotic flow and a small amount of high refractive index organic solvent (glycerol) was added to the sample mixture. It was found that an axially directed laser excitation beam was propagated essentially with total internal reflection, so that minimum interference arose from stray light or from scattering light originating from the wall of the capillary. With the naturally fluorescent protein R-phycoerythrin, a concentration detection limit LOD 10−11 M or mass LOD 10−17 Mo was obtained.

The apparatus for measuring concentrations of fuel mixtures using depth-resolved laser-induced fluorescence is a fluorometer equipped with a sample container holder that is movable in the path of the beam from the light source. Fluorescent emissions from the sample mixture pass at 90° to the excitation light path through a slit that is narrow enough that the emission intensity is effectively produced by a thin layer of the sample and focused on a monochromator, with successive thin layers receiving nonuniform excitation radiation due to reduction of intensity along the excitation light source path with increasing depth penetration and due to reabsorption of emitted fluorescence from adjacent layers. The method has a first mode in which the emission spectrum is scanned at a fixed depth, and a second mode in which the sample is moved relative to the emission monochromator slit to vary the depth while keeping the emission wavelength fixed.

The invention discloses a flow cytometry based on a microfluidic chip, relating to a flow cytometry for biomedical inspection. The structure of the flow cytometry is as follows: a cell sample introduction unit (10), a laser induce fluorescence (LIF) inspection unit (30) and an optical signal inspection and processing unit (50) are respectively connected with a cell sorting chip unit (20); the cell sorting chip unit (20) is connected with lateral and front scattered light inspection units (40); and the LIF inspection unit (30) and the lateral and the front scattered light inspection units (40) are respectively connected with the optical signal inspection and processing unit (50). Compared with the commercial flow cytometry, the flow cytometry has the following advantages and beneficial effects of high integration degree, convenient operation, small volume, high pass, sensitivity and speed, miniaturization, customization and multiple sorting force, and is suitable for individual flow cytometric analysis.

The invention discloses a paper-based micro-fluidic immunosensor chip and a timely field detection immunoassay platform. According to the paper-based micro-fluidic immunosensor chip, a micro-fluidic channel is constructed on a paper base, and a reaction area in the channel is subjected to surface modification, so that chemical crosslinking is carried out on the channel and an antibody, and the antibody is fixed on the surface of the paper base. The chip washing is needed to be combined with a ring oven, the washing and flow velocity are controlled by the ring temperature, and the problems of the conventional paper-based micro-fluidic chips at home and abroad on washing and flow velocity control are solved. Another cluster marker labeled antibody which is specifically combined with an antigen and takes mesoporous silica or metallic oxide-silica composite nanoparticles as a matrix is detected by adopting manners of mobile phone photographing, image scanning and collecting or laser-induced fluorescence, so that the content of antigen to be detected is detected. According to the paper-based micro-fluidic immunosensor chip, the detection sensitivity is obviously improved.

Various embodiments of a multi-laser system are disclosed. In some embodiments, the multi-laser system includes a plurality of lasers, a plurality of laser beams, a beam positioning system, a thermally stable enclosure, and a temperature controller. The thermally stable enclosure is substantially made of a material with high thermal conductivity such as at least 5 W / (m K). The thermally stable enclosure can help maintain alignment of the laser beams to a target object over a range of ambient temperatures. Various embodiments of an optical system for directing light for optical measurements such laser-induced fluorescence and spectroscopic analysis are disclosed. In some embodiments, the optical system includes a thermally conductive housing and a thermoelectric controller, a plurality of optical fibers, and one or more optical elements to direct light emitted by the optical fibers to illuminate a flow cell. The housing is configured to attach to a flow cell.

The invention relates to the field of two-phase / multi-phase flow measurement, aims to realize the non-intrusive and high precision synchronization acquisition of the flow parameters of a liquid film in (outside) a pipeline and temperature field distribution, and is used for the deep study of a liquid film flow characteristic, a heat transfer characteristic and the like. The employed technical scheme is that according to the liquid film temperature field and flow field simultaneous measurement method based on laser induced fluorescence, when a liquid film with dissolved fluorescent dye forms a dynamic liquid film in a pipeline inner / outer wall or a flat panel, a liquid film fluorescence image is taken through a high speed camera, for a same taken fluorescence image, based on a high speed calibration parameter, the geometric feature of the liquid film fluorescence image is accurately extracted, and flow field characteristics comprising a liquid film thickness and a velocity are obtained. At the same time, based on a calibrated temperature-fluorescence intensity calibration curve, laser intensity calibration and a fluorescence intensity extraction method, a liquid film fluorescence image brightness is accurately extracted, and the instantaneous and time averaged temperature field distribution of the liquid film are obtained. The method is mainly applied to a two-phase / multi-phase flow measurement occasion.

The invention belongs to the diesel spray and combustion visualization research field and discloses a measurement apparatus capable of simultaneously measuring a diesel spray structure and a combustion characteristic based on a laser diagnosis technology and a method thereof. In the past, test researches of a diesel spray characteristic and the combustion characteristic can not be performed simultaneously. In the invention, through using a Mie scattering technology of a laser and a laser induced fluorescence technology (OH-PLIF), the spray structure and the combustion characteristic during diesel combustion can be simultaneously measured and researched. The corresponding apparatus comprises a laser system, a constant volume combustion bomb system, a fuel oil supply system, a signal synchronization system and a signal acquisition system. The test apparatus can simultaneously carry out test researches of a liquid phase length of diesel spray and OH-PLIF distribution under a combustion condition, simultaneously can indicate a liquid phase area in a diesel combustion OH-PLIF distribution map and solves an interference problem of a liquid phase signal to a laser-induced fluorescence signal.

It is an on-line self-correction laser leading fluorescence measurement method and its application in the analysis of chromatogram, capillary electrophoresis and core electrophoresis based on charge couple device. The method in this invention uses laser-leading sample to generate fluorescence characteristics and directly adopts CCD as measurement elements and translates the mock video signals collected by CCD into digital signals. It uses non-sample area light intensity in the video signals as reference signals when the self-correction video fluorescence measures the computer program and processes the video digital signals. It adopts reference correction method and eliminates the background interference signals in the sample light intensity and makes the recorded fluorescence signal data not sensitive to the around light interference and can reduce the background noisy interference signals without adding new hardware.

The invention discloses a two-laser induced fluorescence multi-color detector used for a single-cell electrophoretic chip.The detector is composed of a laser excitation module, a fluorescence collection and detection module, a microimaging module, a signal collection and processing module and a microfluidic chip detection platform and has the functions of performing two-laser induced fluorescence multi-color detection, normal microscopic observation, two-laser spot focusing and detection area alignment observation and the like on a microfluidic chip.By means of the detector, simultaneous detection and quantification of fluorescence substances with different light absorption and fluorescence characteristics, especially fluorescence labeling multi-component activated small molecules, with different excitation and different emission, in single cells of electrophoretic separation of the microfluidic chip can be achieved, and space of relative research and application of laser induced fluorescence detection, the microfluidic chip, single-cell analysis, detection of activated small molecules and the like is greatly expanded.

The invention discloses a multifunctional constant-volume bomb for testing spraying, burning and soot generation characteristics. The multifunctional constant-volume bomb comprises a body and two to six optical windows, wherein the body is simple in structure, and the optical windows is protected by double-layer glass (namely thick pressure-bearing glass and thin thermal-insulation glass) and an air thermal-protective layer, so that a stable high-temperature high-pressure test environment is provided. The multifunctional constant-volume bomb provided by the invention has the characteristics that the structure is compact, the structure is simple, the processing is convenient, the arrangement of an optical system and an ejector is flexible, and light guiding grooves are formed in fixators of the optical windows and are suitable for continuously measuring by using a phase Doppler method under a high-temperature and high-pressure condition; the constant-volume bomb provided by the invention is used for applying one and a combination of a plane laser induced fluorescence method (spraying section fuel oil droppoint distribution and the like), a laser induced phosphorescence method (temperature distribution of a gas-phase and a wall surface), a laser absorbing and scattering method (gas-liquid two-phase concentration field, air-fuel ratio distribution and the like), a laser induced flaming method (soot), a schlieren method on the spraying, burning and soot generation characteristics and the like, simultaneously measuring the spraying, burning and soot generation characteristics and the like, and the multifunctional constant-volume bomb has a universality property.

The invention discloses a detection system and a detection method of petroleum-type pollutants. The detection system comprises an emitting unit, a receiving unit, a reference unit and a signal processing unit. The emitting unit is used for emitting laser signals, and transmitted light signals and reflected light signals are outputted; an input terminal of the reference unit receives the transmitted light signals emitted from the emitting unit. An output terminal of the reference unit is connected to an input terminal of the signal processing unit. Reference signals outputted by the reference unit are transmitted to the signal receiving unit by the output terminal of the reference unit. A sample in a sample tank is excited by the reflected light signals, such that fluorescence signals are generated. The fluorescence signals are received by the receiving unit beside the sample tank, and are outputted to the signal processing unit by the receiving unit. The fluorescence signals are processed by the signal processing unit. The system and the method provided by the invention are based on laser-induced fluorescence detection principles. Emitted signals are modulated by using a modulation technology, such that the detection sensitivity of the system is high. With the method and the system, rapid, real-time, on-line and non-contact measuring upon water body petroleum-type pollutant fluorescence signals can be realized. The system and the method can be widely applied in water body environment monitoring.

The invention relates to a high-throughput detection system for microbes based on a droplet microfluidic chip. The system mainly comprises a droplet microfluidic chip system (1), a light path system (2) and a data acquisition and analysis system (3), wherein the droplet microfluidic chip system (1) embeds to-be-detected microbe to an independent single droplet micro-reaction chamber; the light path system (2) is used for carrying out laser-induced fluorescence detection signal transmission of the microbe sample in the single droplet micro-reaction chamber; and the data acquisition and analysis system (3) carries out detection and analysis on the acquired signals through a computer software. The droplet microfluidic chip can be replaced. The system is suitable for laser-induced fluorescence detection and analysis of different types of microbes, has the advantages of simple design, low processing and manufacturing cost, low detection cost, fast speed and high throughput, and realizes efficient screening of production-related target enzymes and metabolites of different types of industrial microbes including escherichia coli, corynebacterium glutamicum and saccharomyces cerevisiae.

The invention discloses a real-time microbial particle counter comprising a light path, an air channel intersected with the light path, and a signal processing system connected with the light path, wherein the light path comprises a lighting light path for irradiating tested particles and a collecting light path arranged along the advancing direction of the lighting light path; the collecting light path also comprises a relay system for separating the light path and separately detecting separated light paths; the air channel is used for sampling the tested particles; the signal processing system is used for analyzing and processing a signal, and comprises a fluorescent preamplifier and a scattered light preamplifier. The concentration of microbial particles in air is monitored in real time by taking laser-induced fluorescence detection as the principle, the particle sizes and the biological attributes of the tested particles are judged by detecting the intensities of scattered light and fluorescence emitted by exciting light, and the particles in a sampling airflow are counted, so that the automatic detection on planktonic bacteria microorganisms is achieved, and the real-time microbial particle counter is simple and convenient to operate, strong in detection instantaneity, high in sensitivity and high in accuracy.

Provided is a method for direct quantification of gene expression using capillary electrophoresis with laser-induced fluorescence to measure RNA in a sample. Also provided is a method of diagnosing a disease in a subject, wherein the disease is caused by increased or decreased expression of a causative gene.

The invention provides a device and method capable of simultaneously measuring the velocity field and the concentration field of fluid and especially suitable for online measuring of the velocity field and concentration field during fluid mixing. The method includes: simultaneously adding tracer particles and fluorescent reagent into to-be-measured fluid or one fluid of mixed fluid, using a particle image velocity measuring method to measure the velocity field, and using a laser-induced fluorescence method to measure the concentration field. A light splitting device is arranged in front of a measuring camera, and a laser-irradiated image part and an induced-fluorescence-irradiated image part are formed in the same image through light splitting, filtering and light path convergence. The shooting object of the two images is the same, but images of different wavelength ranges are displayed. The laser-irradiated image part is used to perform velocity field analysis, and the induced-fluorescence-irradiated image part is used to perform concentration field analysis. The device and method has the advantages that the velocity field and the concentration field are measured simultaneously by using one measuring camera, and the problem that multi-camera shooting is poor in synchronism, complex in structure and high in cost is solved.

Various embodiments of an optical system for directing light for optical measurements such laser-induced fluorescence and spectroscopic analysis are disclosed. In some embodiments, the optical system includes a thermally conductive housing and a thermoelectric controller, a plurality of optical fibers, and one or more optical elements to direct light emitted by the optical fibers to illuminate a flow cell. The housing is configured to attach to a flow cell.

The invention belongs to the technical field of fluid measurement, and discloses a PIV (Particle Image Velocimetry)-based mixed liquid flow field and concentration measuring device, and a method of using the device for measuring the flow field or the concentration. The measuring device comprises a main water box, an experimental box body, a buffer tank, a water storage box and a PIV system, wherein the main water box is divided into an A-region box body and a B-region box body for providing liquid of a mixed pipeline and liquid of a main pipeline respectively, the liquid is mixed in the experimental box body, and measurement on the flow filed or the concentration is carried out via the PIV system. Tracer particles are sprayed in water in the main water box and the buffer tank, and the flow field of the mixed liquid is measured via the PIV system; fluorescent particles are added to the two box bodies of the main water box respectively for preparing different concentrations, and the concentration of the mixed liquid can be measured via the PIV system in combination with a LIF (Laser Induce Fluorescence) system; measurement on the mixed flow field and the mixed concentration can be carried out in the same device, water and tracer particles can be saved, and the flow field and the concentration can be detected in real time.

On a conventional T-shaped glass chip, a permanent magnet is utilized to fix and decorate a nanometer magnetic bead of a DNA probe in a separation channel of a chip; the rapid, sensitive, high-specific, high-stability, low-cost separation detection on the DNA is realized by utilizing electric drive sampling and combining a laser induced fluorescence detector or an electrochemical detector, thus solving the defects that the time of separating and detecting the DNA by utilizing a microfluidic chip is long, the separation effect is poor, the sensitivity is low, the specific is poor and the stability is poor and the like; the interface manufacturing is avoided by an electric sampling manner; the influence of dead volume is removed; and the sample use amount is reduced in a narrower and uniform micro pipeline, and meanwhile the lower detection limit is ensured.

A laser remote sensing apparatus comprises a laser to provide collimated excitation light at a wavelength; a sensing optic, comprising at least one optical element having a front receiving surface to focus the received excitation light onto a back surface comprising a target sample and wherein the target sample emits a return light signal that is recollimated by the front receiving surface; a telescope for collecting the recollimated return light signal from the sensing optic; and a detector for detecting and spectrally resolving the return light signal. The back surface further can comprise a substrate that absorbs the target sample from an environment. For example the substrate can be a SERS substrate comprising a roughened metal surface. The return light signal can be a surface-enhanced Raman signal or laser-induced fluorescence signal. For fluorescence applications, the return signal can be enhanced by about 105, solely due to recollimation of the fluorescence return signal. For SERS applications, the return signal can be enhanced by 109 or more, due both to recollimation and to structuring of the SERS substrate so that the incident laser and Raman scattered fields are in resonance with the surface plasmons of the SERS substrate.

The laser-induced fluorescence confocal scanning device and method belongs to the biochip detection technology field. The fluorescence collection and usage efficiency of existing devices is low, and meanwhile the focusing control in the scanning process is very difficult. The invention uses the middle hole full reflection mirror to optical divide the incident laser and the induced reflected fluorescence, and when a laser beam passing through the aperture, the energy loss is small, almost all laser energy can be irradiated to the biochip, and when collecting the fluorescence, the fluorescence collection angle is big, not only improving the efficiency of laser incident, but also improving the fluorescence collection efficiency. Using computer control, it can maintain approximately confocal relation between the light source pinhole and the detector pinhole, and only need focus one time before scanning, making the focusing control easy and convenient.

The invention is a laser-inducing-fluorescence detector. It includes: a piece of inching equipment to plant and ajdust the position of sample holder; an aided light source to adjust the light path; an observation mirror for adjusting the light path; an exciting light source as its working light source made up of the laser, the reference photoelectric diode and light-reducing board and the dichroiscope; a group of optical lenses in imagine and focus the working light source, made up of the focus lens, the imagining lens, the light-filtering piece and the light diaphragm; and a photoelectric signal converter to receive and output the detecting signal. The invention is based on the common-focus mode to get the high-efficiency imagining system and it can be used in the separating-analysis technique such as Micro-HPLC, CE, CEC and so on.

The invention provides a droplet capture chip. The droplet capture chip has the beneficial effects that a plurality of droplet capture chambers are arranged in the droplet capture chip, so that droplets can directly undergo in situ amplification and in situ detection after being captured; droplet generation and droplet capture are integrated on one microfluidic chip, so that convenient and rapid totally integrated automation detection is achieved, the intermediate links can be reduced and the analysis speed can be increased; the imaging information of the droplet capture chip is directly captured with a CCD (charge-coupled device), the CCD is simple and is easy to operate, and the accuracy rate of the obtained result is high; high-sensitivity detection can be achieved by scanning each fixed droplet by laser-induced fluorescence; the droplet capture chip and the microfluidic chip can make up for the deficiency of the detection technology of existing blood circulation DNA mutation, can achieve more sensitive simultaneous detection of multiple mutations and have great significance in increasing the clinical application value of blood circulation DNA in tumor diagnosis and therapy.

The invention relates to a laser induced fluorescence and optical absorption double-function detection microfluid control electrophoretic chip. It includes chip base plate and cover plate which are superimposed and bonded into one body and made up by using transparent material. On the base plate the crossed microfluid channels (sample channel and separation channel) are cut; and on the cover plate and in the place correspondent to the tail end position of microfluid channel on the base plate the small-hole type liquid storage cells (sample cell, sample waste liquor cell, buffer cell and waste liquor cell) are set; and on the cover plate or base plate the optical fibre channels are set, these optical fibre channels are close to tail end of the separation channel, perpendicular to said separation channel and positioned at its two sides.

The invention belongs to the field of internal combustion engine researches and discloses a natural gas / diesel oil dual fuel injection mixed characteristic research device and a control method thereof. The device comprises an injection combiner, a constant volume bomb, a natural gas injector, a diesel oil injector, a gas supply system, an oil supply system, a computer I, an ECU, a laser, a synchronizer, a camera I, a camera II, a reflector and a spectroscope. The natural gas injector and the diesel oil injector are fixedly installed in the injection combiner. The injection combiner is installed at the top of the constant volume bomb. The gas supply system is connected with the natural gas injector. The oil supply system is connected with the diesel oil injector. The ECU controls the natural gas injector and the diesel oil injector to be started or stopped. The computer I controls the laser, the camera I and the camera II to work synchronously through the synchronizer. According to the natural gas / diesel oil dual fuel injection mixed characteristic research device and the control method thereof, dual fuel injection mixing is achieved, clear mist form images are captured through a laser induced fluorescence technique, and a heating unit, a temperature and pressure sensor and an electromagnetic flowmeter are arranged in a pipeline so as to research the influence of the state of fuel on the mist mixed characteristics.