Real-time microbial particle counter

Abstract

The invention discloses a real-time microbial particle counter comprising a light path, an air channel intersected with the light path, and a signal processing system connected with the light path, wherein the light path comprises a lighting light path for irradiating tested particles and a collecting light path arranged along the advancing direction of the lighting light path; the collecting light path also comprises a relay system for separating the light path and separately detecting separated light paths; the air channel is used for sampling the tested particles; the signal processing system is used for analyzing and processing a signal, and comprises a fluorescent preamplifier and a scattered light preamplifier. The concentration of microbial particles in air is monitored in real time by taking laser-induced fluorescence detection as the principle, the particle sizes and the biological attributes of the tested particles are judged by detecting the intensities of scattered light and fluorescence emitted by exciting light, and the particles in a sampling airflow are counted, so that the automatic detection on planktonic bacteria microorganisms is achieved, and the real-time microbial particle counter is simple and convenient to operate, strong in detection instantaneity, high in sensitivity and high in accuracy.

Description

technical field [0001] The invention belongs to the field of suspended microorganism detection, in particular to a real-time microorganism particle counter. Background technique [0002] Microorganisms such as infectious bacteria or viruses exist in the atmosphere in the form of aerosol particles. When the concentration of infectious bacteria or viruses in the atmosphere exceeds a certain threshold, it will pose a threat to the health of humans, animals and plants. At the same time, because microorganisms can produce various dormant bodies, they can survive in the air for a long time, and spread and transmit with the help of air medium, which will lead to the outbreak and spread of various infectious diseases and cause serious harm. Therefore, there is an urgent need for technologies and equipment that can detect microbial particles in the surrounding environment in real time. [0003] Microbial particles contain a variety of organic molecules, among which the main fluoresc...

AI Technical Summary

Problems solved by technology

[0009] (2) The detection cycle is long and not real-time

Therefore, this method cannot be applied to applications that require high real-time performance, such as biological weapons early warning

[0011] (3) The false negative rate is high

[0012] The planktonic bacteria sampling culture method counts the microorganisms after culturing, but the growth cycle of different microorganisms is not the same, so it is difficult to ensure complete statistics of the number of all microorganisms

During the incubation time, some microorganisms may not have multiplied to a countable degree, and some microorganisms may have died

In addition, there is a large number of non-culturable microorganisms in the air, which cannot form colonies on the medium and cannot be counted by planktonic sampling culture method

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