The invention provides an identification method for existence of
fungus in
ganoderma lucidum tissue and an assessment method for necessity of
fungus for formation of
ganoderma lucidum. The identification method comprises the steps of a, measuring the dissolving temperature of a
ganoderma lucidum tissue block; b, preparing a
ganoderma lucidum tissue dissolving solution; c, filtering the
ganoderma lucidum tissue dissolving solution with a
disposable needle filter, so as to enrich the
fungus that cannot pass through a filter membrane on the filter membrane, and
mycoplasma is retained in the filter liquor by passing through the filter membrane; d, taking out the filter membrane in step c and
cutting up the filter membrane, then extracting
DNA by using a CTAB method; e, selecting respective
positive control fungus of five fungi, and
processing by the same method as steps b to d; f, identification of the existence of the five fungi: identifying the
mycoplasma by taking the filter liquor as an amplification template, and identifying the other four fungi by taking the
DNA extracted from the
thallus on the filter membrane as an amplification template. By applying the identification method provided by the invention, the existence of the five fungi can be measured, the fungus constitution information in the
ganoderma lucidum can be accurately acquired, and a foundation is laid for quicklyand more accurately verifying the
biological attributes.