Flow cytometry based on microfluidic chip

Abstract

The invention discloses a flow cytometry based on a microfluidic chip, relating to a flow cytometry for biomedical inspection. The structure of the flow cytometry is as follows: a cell sample introduction unit (10), a laser induce fluorescence (LIF) inspection unit (30) and an optical signal inspection and processing unit (50) are respectively connected with a cell sorting chip unit (20); the cell sorting chip unit (20) is connected with lateral and front scattered light inspection units (40); and the LIF inspection unit (30) and the lateral and the front scattered light inspection units (40) are respectively connected with the optical signal inspection and processing unit (50). Compared with the commercial flow cytometry, the flow cytometry has the following advantages and beneficial effects of high integration degree, convenient operation, small volume, high pass, sensitivity and speed, miniaturization, customization and multiple sorting force, and is suitable for individual flow cytometric analysis.

Description

technical field [0001] The invention relates to a flow cytometer for biomedical detection, in particular to a flow cytometer based on a microfluidic chip. Background technique [0002] Flow Cytometer (FCM for short) is a new high-tech instrument integrating laser technology, electron physics technology, photoelectric detection technology, computer technology, cell fluorescence chemistry technology and monoclonal antibody technology. Flow cytometry is a technique for multi-parameter, rapid quantitative analysis and sorting of cells and biological particles in a fast linear flow state. The structure of a flow cytometer generally includes four parts: (1) flow chamber and liquid flow drive system; (2) optical system; (3) signal detection and storage, analysis, and display system; (4) cell sorting system. The principle of cell sorting is: the sheath flow forms a signal of a certain frequency, and this signal is added to the piezoelectric crystal to generate mechanical vibrations...

AI Technical Summary

Problems solved by technology

At present, commercial flow cytometers are large in size, complex in structure, complete in function, and single in sorting means. In view of this and the advantages of microfluidic chip technology, it is hoped that there will be a new type of miniaturized flow cytometer based on microfluidic chip technology. cytometer, or even a dedicated microfluidic chip flow cytometer for a certain biochemical analysis function

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