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High-throughput detection system for microbe based on droplet microfluidic chip

A microfluidic chip and detection system technology, applied in the field of high-throughput detection systems for microorganisms, can solve the problems of slow progress in the research of intermittent droplets, and achieve the effects of fast speed, simple system design, and high-efficiency screening

Inactive Publication Date: 2014-08-27
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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Problems solved by technology

Compared with continuous fluid systems, the research progress of intermittent droplets is slow, and there are no related commercial instruments

Method used

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  • High-throughput detection system for microbe based on droplet microfluidic chip
  • High-throughput detection system for microbe based on droplet microfluidic chip

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Embodiment Construction

[0013] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0014] as attached figure 1 The high-throughput detection system of the droplet microfluidic chip shown in the present invention includes a droplet microfluidic chip system (1), an optical path system (2) and a data acquisition and analysis system (3). The droplet microfluidic chip system (1) and the optical path system (2) are fixed on a two-dimensional platform; the data acquisition and analysis system (3) is connected to a computer to detect and analyze the signals collected from the microfluidic chip . The microbial sample to be detected is embedded in the droplet microfluidic chip system (1) to form an independent single-droplet micro-reaction chamber, and then the microbial sample in the single-droplet micro-reaction chamber is detected by the optical path system (2). The laser-induced fluorescence detection signal is transmitted, specif...

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Abstract

The invention relates to a high-throughput detection system for microbes based on a droplet microfluidic chip. The system mainly comprises a droplet microfluidic chip system (1), a light path system (2) and a data acquisition and analysis system (3), wherein the droplet microfluidic chip system (1) embeds to-be-detected microbe to an independent single droplet micro-reaction chamber; the light path system (2) is used for carrying out laser-induced fluorescence detection signal transmission of the microbe sample in the single droplet micro-reaction chamber; and the data acquisition and analysis system (3) carries out detection and analysis on the acquired signals through a computer software. The droplet microfluidic chip can be replaced. The system is suitable for laser-induced fluorescence detection and analysis of different types of microbes, has the advantages of simple design, low processing and manufacturing cost, low detection cost, fast speed and high throughput, and realizes efficient screening of production-related target enzymes and metabolites of different types of industrial microbes including escherichia coli, corynebacterium glutamicum and saccharomyces cerevisiae.

Description

Technical field [0001] The invention relates to a high-throughput detection system based on a droplet microfluidic chip, in particular to a high-throughput detection system for microorganisms based on laser-induced fluorescence detection of a droplet microfluidic chip. Background technique [0002] The microbial high-throughput screening system refers to a technical platform that can automatically, quickly and efficiently screen the functions and products of microorganisms, and is one of the bottlenecks in the development and industrialization of industrial microbial resources. Screening from nature and mutation evolution in the laboratory has always been an important way to discover and innovate industrial microbial strains, and is the basis of the industrial biotechnology industry. The microbial strains used in industry are mainly obtained through mutagenesis screening. With the rapid development of modern metabolic engineering technology and synthetic biology, rational de...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 王钦宏涂然袁会领孙琳
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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