Laser induced fluorescence and light absorption bifunction detecting microflow controlled electrophoresis ehip

A laser-induced fluorescence and electrophoresis chip technology, which is applied in fluorescence/phosphorescence, color/spectral characteristic measurement, measurement devices, etc., can solve the problems of single function and complex structure of optical detection system, and achieves ingenious design and simple operation of optical path. , the effect of benefiting the resolution and detection sensitivity

Inactive Publication Date: 2005-03-09
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The purpose of the present invention is to overcome the defects of the single function of the microfluidic electrophoresis chip in the prior art and the complex structure of the required optical detection system, and provide a fiber-coupled microfluidic chip that can simultaneously realize laser-induced fluorescence detection and ultraviolet-visible light absorption detection. Electrophoresis

Method used

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  • Laser induced fluorescence and light absorption bifunction detecting microflow controlled electrophoresis ehip
  • Laser induced fluorescence and light absorption bifunction detecting microflow controlled electrophoresis ehip
  • Laser induced fluorescence and light absorption bifunction detecting microflow controlled electrophoresis ehip

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Example 1: Fabrication of fiber-coupled microfluidic electrophoresis chip made of glass

[0060] 1. Fabrication of chip substrate 2:

[0061] The size design of the microchannel on the mask film: the width of the fluid channel is 40 μm; the width of the fiber channel is 60 μm; the distance d between the fiber channel and the separation channel CD is 200 μm;

[0062] Place the mask film on a 63mm×63mm×1.5mm uniform chrome plate, expose to ultraviolet rays for 180 seconds (wavelength 365nm), develop in the developer for 100 seconds, and dry at 100°C for half an hour;

[0063] Corrode the chromium film with a chromium film etching solution (cerium sulfate: perchloric acid: water=50 grams: 15 milliliters: 300 milliliters) at room temperature, then rinse it with high-purity water, and dry it;

[0064] The channel size on the chromium plate was measured by digital microscope photography: the width of the fluid channel is 60 μm; the width of the optical fiber channel is 90 μm...

Embodiment 2

[0074] Example 2: Fabrication of PMMA laser-induced fluorescence and light absorption dual-function detection microfluidic electrophoresis chip

[0075] 1. Design of the glass positive template mask film (substrate and cover sheet): the width of the microfluidic channel on the positive template mask of the chip substrate is 120 μm; the width of the optical fiber channel on the positive template mask of the chip cover plate is 250 μm, The distance is 240 μm.

[0076] 2. Production of substrate positive template: place the chip substrate mask film on a 63mm×63mm×1.5mm uniform chrome plate, expose to ultraviolet rays for 5 minutes (wavelength 365nm), develop in the developer for 100 seconds, and bake at 100°C half an hour. The chromium film was etched with a chromium film etching solution (cerium sulfate: perchloric acid: water = 50 g: 15 ml: 300 ml) at room temperature, and then rinsed with water. With 0.2M HF / 0.2M NH 4 F etchant was used for etching, the rate was about 4 μm / ...

Embodiment 3

[0081] Example 3: Fabrication of a microfluidic electrophoresis chip for dual-function detection of laser-induced fluorescence and light absorption made of PDMS

[0082] 1. The design of the glass positive template mask film (substrate and cover sheet): the width of the microfluidic channel on the positive template mask of the substrate is 120 μm; the width of the optical fiber channel on the positive template mask of the cover plate is 250 μm, and the distance between the two optical fiber channels 240μm;

[0083] 2. Production of glass substrate positive template: place the substrate mask film on a uniform chrome plate of 63mm×63mm×1.5mm, expose to ultraviolet rays for 5 minutes (wavelength 365nm), develop in the developer for 100 seconds, and bake at 100°C half an hour. The chromium film was etched with a chromium film etching solution (cerium sulfate: perchloric acid: water = 50 g: 15 ml: 300 ml) at room temperature, and then rinsed with water. With 0.2M HF / 0.2M NH 4 F ...

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Abstract

The invention relates to a laser induced fluorescence and optical absorption double-function detection microfluid control electrophoretic chip. It includes chip base plate and cover plate which are superimposed and bonded into one body and made up by using transparent material. On the base plate the crossed microfluid channels (sample channel and separation channel) are cut; and on the cover plate and in the place correspondent to the tail end position of microfluid channel on the base plate the small-hole type liquid storage cells (sample cell, sample waste liquor cell, buffer cell and waste liquor cell) are set; and on the cover plate or base plate the optical fibre channels are set, these optical fibre channels are close to tail end of the separation channel, perpendicular to said separation channel and positioned at its two sides.

Description

technical field [0001] The invention belongs to the technical field of electrophoresis chips, and in particular relates to a fiber-coupled microfluidic electrophoresis chip capable of simultaneously realizing laser-induced fluorescence detection and ultraviolet-visible light absorption detection; the chip is suitable for fluorescent substances or biochemical substances that can be marked by fluorescent reagents The separation and detection of samples such as amino acids, proteins, and DNA can also be used for the separation and detection of some non-fluorescent samples that have strong absorption in the ultraviolet-visible region. The chip can detect many types of samples and has a wide range of applications. Background technique [0002] In the early 1990s, the introduction of the concept of Micro-total analysis system (μ-TAS) had a major impact on the field of analytical instruments and analytical science, leading the trend of chemical analysis equipment towards miniaturiz...

Claims

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Application Information

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IPC IPC(8): G01N21/25G01N21/3577G01N21/64G01N27/447
Inventor 林金明李海芳
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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