Liquid compound biological preservative and preparation method and application thereof
A technology of biological preservatives and composite bacterial agents, which is applied in the field of liquid composite biological preservatives and its preparation, and can solve problems such as human health hazards
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preparation example Construction
[0088] The preparation method of the liquid compound biological antistaling agent of embodiment is the following steps:
[0089] The preparation method of A, chitosan, epsilon-polylysine, lysozyme:
[0090] A, the preparation method of chitosan:
[0091] Weigh 25 grams of shrimp shells, add 250 ml of 1 mol / L hydrochloric acid solution, stir, let stand for 12 hours, filter, discard the filtrate; add 5% sodium hydroxide solution to a solid-liquid ratio of 1:8, stir, and separate Boil in water for 1 hour, filter to obtain chitin, dry, crush, and set aside;
[0092] Take 5 grams of chitin powder in a three-necked flask, add 100 ml of 45% sodium hydroxide solution, stir and react at 115°C for 1 hour, cool, centrifuge, remove the supernatant, wash the precipitate with water, centrifuge again, and remove the supernatant solution, washed the precipitate with water, then removed the supernatant, washed the precipitate with 95% ethanol, poured it into a suction filter bottle, filtered...
Embodiment 1
[0125] Embodiment 1: be made up of the raw material of following weight portion proportioning:
[0126] 10 parts of chitosan, 10 parts of ε-polylysine, 10 parts of lysozyme, 7.5 parts of bacterial liquid of Streptomyces hygroscopicus, 7.5 parts of bacterial liquid of Streptomyces luteus, 15 parts of bacterial liquid of Streptomyces aureochromogenes, 15 parts of Streptococcus lactis, 10 parts of Bacillus subtilis, 10 parts of Bacillus licheniformis, 7.5 parts of Saccharomyces cerevisiae, 7.5 parts of Paecilomyces lilacinus, Trichoderma harzianum 7.5 parts of bacteria solution.
Embodiment 2
[0127] Embodiment 2: be made up of the raw material of following weight portion proportioning:
[0128] 20 parts of chitosan, 7.5 parts of ε-polylysine, 7.5 parts of lysozyme, 10 parts of bacterial liquid of Streptomyces hygroscopicus, 10 parts of bacterial liquid of Streptomyces luteus, 10 parts of bacterial liquid of Streptomyces aureochromogenes, 10 parts of Streptococcus lactis, 10 parts of Bacillus subtilis, 10 parts of Bacillus licheniformis, 6 parts of Saccharomyces cerevisiae, 6 parts of Paecilomyces lilacinus, Trichoderma harzianum 6 parts of bacterial solution.
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