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Streptomyces angustmyceticus and application of Streptomyces angustmyceticus in prevention and treatment of plant oomycetes and fungal diseases as well as promotion of plant growth

A technology for plant fungal diseases and hygroscopic Streptomyces, which is applied in plant growth regulators, plant growth regulators, botanical equipment and methods, etc., and can solve the problems of low stability, slow effect, and biological control drugs or measures that have not been widely promoted. To achieve the effect of easy preservation, simple culture conditions, and good development and application prospects

Active Publication Date: 2020-04-03
CHONGQING UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as an emerging science, biological control also has many shortcomings, such as: compared with chemical fungicides, its stability is low and its effect is slow
Biocontrol drugs or measures currently on the market have not been widely promoted

Method used

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  • Streptomyces angustmyceticus and application of Streptomyces angustmyceticus in prevention and treatment of plant oomycetes and fungal diseases as well as promotion of plant growth
  • Streptomyces angustmyceticus and application of Streptomyces angustmyceticus in prevention and treatment of plant oomycetes and fungal diseases as well as promotion of plant growth
  • Streptomyces angustmyceticus and application of Streptomyces angustmyceticus in prevention and treatment of plant oomycetes and fungal diseases as well as promotion of plant growth

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, isolation and identification of bacterial strain

[0026] 1. Isolation and screening of strain CQUSa03

[0027] In the potato planting area of ​​Wuxi County, Chongqing, according to the occurrence of late blight, select an appropriate location to collect rhizosphere soil at a distance of 5-20mm, and mark the sampling time, location, vegetation and disease occurrence. The soil dilution method was used to isolate on Gao's No. 1 medium, and after 48 hours of constant temperature cultivation at 28°C, a single colony was picked and preserved as the strain to be tested. For the selected strains to be tested, the plate confrontation method was used to inoculate Phytophthora infestans in the center of the rye medium (the pathogen was cultivated at 20°C for 10 days, and the diameter of the bacterial cake was 0.6cm), and the surrounding bacteria were inoculated symmetrically, and each strain was repeated. 3 times, observe whether there is a bacteriostatic zone. Th...

Embodiment 2

[0031] Example 2, CQUSa03 confrontation experiment with potato late blight pathogen and other pathogenic fungi

[0032] Preparation of rye medium: Weigh 60g of rye flour, add 500mL of sterile water and soak for 36 hours. Filtrate to obtain filtrate 1, save it for later use; filter the filter residue in a water bath at 55°C for 3 hours to obtain filtrate 2; mix filtrate 1 and filtrate 2, sterilize at 121°C for 10 minutes and filter to obtain filtrate 3; add 20g sucrose and 12g agar to the filtrate 3 Dissolve and mix well, dilute to 1L with sterile water, and sterilize at 121°C for 20 minutes.

[0033] PDA medium preparation: After adding 20 g of glucose and 15 g of agar powder to the filtrate of 200 g of fresh potatoes boiled, the volume was adjusted to 1 L, and sterilized at 121°C for 15 minutes.

[0034]Phytophthora physiological races mainly have three types: A1, A2 and self-fertile. The international standard strain T30-4 used in this patent belongs to type A1 and is deri...

Embodiment 3

[0042] Embodiment 3, the liquid bacterial agent containing bacterial strain CQUSa03 and preparation method thereof

[0043] The liquid inoculum containing the above-mentioned bacterial strain CQUSa03 provided by the present embodiment is prepared by the following method:

[0044] (1) Strain activation: the strain CQUSa03 was inoculated on oat solid medium and cultured at 28°C for 7 days. The formula of oat solid medium is: oat 2%, agar powder 2%.

[0045] (2) Seed culture: inoculate the activated strain into the ISP liquid medium, culture at 28°C, 200 rpm, for 5 days, and obtain the seed medium. The formula of ISP liquid medium is: maltose extract 1%, yeast extract 0.4%, D-glucose 0.4%, pH 7.0-7.4.

[0046] (3) Liquid fermentation: Add the seed medium obtained in step (2) to the fermentation medium at 10% (volume percentage), fermentation temperature 28°C, tank pressure 0.07Mpa, stirring rate 100rpm, oxygen flow>60% , fermentation time 7d. The formula of the fermentation m...

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Abstract

The invention discloses a Streptomyces angustmyceticus strain CQUSa03, and a preservation number is CGMCC No. 18715. The invention also discloses a biological agent taking the strain CQUSa03 as an active ingredient. The invention also discloses an application of the strain CQUSa03 in prevention and treatment of plant oomycetes and plant fungal diseases, and an application of the strain CQUSa03 inpromotion of plant growth. The invention also discloses a preparation method of a liquid inoculant containing the strain CQUSa03. The preparation method comprises the following steps: (1) activating the strain; (2) performing seed culture; and (3) performing liquid fermentation; adding a seed culture medium into a fermentation culture medium for fermentation to obtain a liquid microbial agent; wherein a formula of the fermentation medium comprises 4% of glucose, 1% of soybean cake powder, 0.2% of amino acid powder, 0.5% of K2HPO4, 0.5% of KH2PO4, 0.5% of NaCl, 0.15% of ferrous sulfate, 0.2% ofan antifoaming agent and a pH value is 7.2-7.4.

Description

technical field [0001] The invention relates to a microorganism and its application, in particular to a Streptomyces hygroscopicus stenomycin subspecies and its application in preventing and treating plant oomycetes and fungal diseases and promoting plant growth. Background technique [0002] Potato is a dicotyledonous plant belonging to Solanaceae, and it is the fourth largest staple food crop in the world after rice, wheat and corn. However, during its growth and development, potatoes have been threatened by more than 30 kinds of diseases and insect pests, causing serious economic losses, the most typical of which are potato late blight, early blight, fusarium wilt and dry rot. [0003] Potato late blight is an oomycete disease of potato stem and leaf death and tuber rot caused by Phytophthora infestans, and is one of the most devastating diseases in potato production. Rapid expansion can cause large-scale disease in the field in a relatively short period of time, resulti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/28A01P1/00A01P3/00A01P21/00C12R1/55
CPCA01N63/00C12N1/205C12R2001/55
Inventor 罗秀媚田婷婷任茂智李正国王中康黄晓清谭雪董攀
Owner CHONGQING UNIV
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