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Application of microorganism bacterium agent in resisting of potato scab

A technology of potato scab, microbial agent, applied in the field of agriculture and biology

Inactive Publication Date: 2015-06-10
DALIAN SANKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is to develop a microbial agent capable of efficiently antagonizing potato scab. Using the interaction between microorganisms, equipped with high-tech production fermentation technology to produce high-quality products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the preparation of fermented liquid

[0020] 1) Inoculation: prepare solid culture medium of various strains, carry out strict aseptic operation after sterilization, transfer from inclined plane to flat plate, and cultivate at optimum temperature for 2 to 3 days;

[0021] 2) Primary culture: Pick a single colony from the well-grown plate and transfer it to a 50mL Erlenmeyer flask filled with primary liquid medium, perform aseptic operation, and culture at 30°C and 130r / min for 24-48 hours;

[0022] 3) Secondary fermentation: Inoculate the primary culture strains into a 500mL Erlenmeyer flask filled with secondary liquid medium, perform aseptic operation, and culture at 30°C and 130r / min for 24-48h to obtain Bacillus subtilis ( Bacillus subtilis) CGMCC1.3382 fermentation broth, Dactylosporangium sp. ACCC No.40661 fermentation broth, Streptomyces roseoflavus ACCC No.40400 fermentation broth.

[0023] Medium used in the above method:

[0024] The solid cult...

Embodiment 2

[0027] Embodiment 2: Optimization of fermentation strain culture conditions

[0028] For the Bacillus subtilis (Bacillus subtilis) CGMCC1.3382 fermentation liquid obtained by culturing in Example 1, the actinomycete (Dactylosporangium sp.) ACCC No.40661 fermentation liquid, and the Streptomyces roseoflavus (Streptomyces roseoflavus) ACCC No.40400 fermentation liquid were respectively Mix according to the mass percentage of 30-35%, 30-35%, 33-38%, and inoculate the mixed bacterial solution in the fermenter containing the fermentation medium, the inoculation amount is 1-5% of the total fermentation medium mass. %, cultured at 30°C for 4 to 7 days, so that the number of bacteria in the fermentation broth reached 10×10 8 individual / mL or more, the microbial bacterial agent for antagonizing potato scab can be obtained. Wherein, the fermentation medium is: glucose 15-30g / L, starch 10-15g / L, peptone 15-23g / L, NaCl5-10g / L, KH 2 PO 4 0.1~0.5g / L, the rest is distilled water; pH6.0~8....

Embodiment 3

[0038] Embodiment 3: Strain compound and liquid submerged fermentation

[0039] The various strains are mixed according to the following mass percentages: 30% of Bacillus subtilis fermentation broth, 35% of actinomycetes fermentation broth, and 35% of Streptomyces roseus flavum fermentation broth. Inoculate the mixed bacterial liquid into a fermenter filled with fermentation medium, the inoculum amount is 3% of the total fermentation medium mass, and cultivate at 30°C for 5 days, so that the number of bacteria in the fermentation liquid reaches 10×10 8 individual / mL or more, the microbial bacterial agent for antagonizing potato scab can be obtained. Wherein, the fermentation medium is: glucose 15-30g / L, starch 10-15g / L, peptone 15-23g / L, NaCl5-10g / L, KH 2 PO 4 0.1~0.5g / L, the rest is distilled water; pH6.0~8.0.

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Abstract

The invention discloses application of a microorganism bacterium agent in resisting of potato scab. According to the microorganism bacterium agent, Bacillus subtilis CGMCC1.3382, Dactylosporangium sp. ACCC No.40661 and Streptomyces roseoflavus ACCC No.40400 are used as preparation strains; all the strains are prepared into fermented solutions respectively; and the fermentation solutions are mixed and then are deeply fermented to obtain the microorganism bacterium agent. The series of the strains reasonably utilize the mutual effect of microorganisms, and the product has the characteristics of strong adaptability, good stability, uneasiness in ageing, strong pathogenic bacteria antagonism and the like; and the pathogenic bacteria of the potato scab can be efficiently resisted, the growth of plants is stimulated and the quality of crops is improved, so that the aims of yield increasing and high yield are realized.

Description

technical field [0001] The invention belongs to the field of agriculture and biotechnology, and relates to a microbial agent for antagonizing potato scab, in particular, to a kind of effective compounding of different strains which can effectively treat potato scab and is produced by synergistic effect. A microbial agent. Background technique [0002] Potatoes are one of the most important crops in my country, second only to rice, wheat and corn, and are the fourth largest crops in my country. Potatoes are grown in a wide range of areas, and the diseases are relatively complex. Among them, potato scab caused by a variety of phytopathogenic Streptomyces is one of the important diseases affecting potato production today. [0003] Potato scab is mainly caused by Streptomyces, whose mycelium is slender and continuously divided to produce a large number of spores. The spores are cylindrical, and the pathogenic bacteria generally invade through the soil stomata from the potato e...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P1/00
Inventor 崔国臣顾艳丽崔昌娜周悦
Owner DALIAN SANKE BIO ENG
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