Method for separating and extracting secondary metabolites of streptomyces

Abstract

The invention belongs to the technical field of metabolite extraction, and discloses a method for separating and extracting secondary metabolites of streptomyces, which comprises the following steps:carrying out elution on a fermentation broth by using an HP-20 resin column, performing chromatographic separation by a silica gel column and a Sephadex LH-20 gel column, and performing analysis and purification of a compound by (semi) preparing a high performance liquid chromatography. Modern instrument spectrum analysis technologies such as nuclear magnetic resonance and high-resolution mass spectrometry are used for identifying the structure of the monomeric compound. According to the invention, actinomycetes which are separated from a soil sample and have antibacterial and anti-tumor cellactivity are fermented, and induction research is carried out on bioactivity of metabolites and part of products generated by the actinomycetes, so that diversity of microbial compounds is enriched; areference and theoretical basis is provided for research and exploration of active substances produced by other actinomycetes; meanwhile, a premise is provided for exploring and developing active natural compounds and commercial drugs.

Description

technical field [0001] The invention belongs to the technical field of metabolite extraction, and in particular relates to a method for separating and extracting secondary metabolites of Streptomyces. Background technique [0002] Currently, the closest prior art: [0003] Due to the abuse of antibiotics and the continuous emergence of drug-resistant strains, and 60% to 70% of clinically used anticancer drugs and antibiotics are derived from or derived from their precursor natural products, people have been committed to discovering new active substances, and Actinomycetes are recognized as the largest group of microorganisms that produce active substances, and they are also the most studied ones. However, due to the limitation of research methods and methods, the isolated actinomycetes only account for 10% of all actinomycetes in the soil. Among them, Streptomyces producing biologically active substances, especially antibiotics, accounts for half of the many actinomycetes, ...

AI Technical Summary

Problems solved by technology

[0004] To sum up, the problem existing in the existing technology is that it is becoming more and more difficult to isolate active compounds with novel structures from microorganisms, and it is urgent to develop methods that can isolate and identify novel compounds faster and more easily.

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