Method for promoting growth of rice and promoting rice to absorb iron element in iron deficiency environment
An iron deficiency and iron technology, applied in the field of maintaining the growth of rice, can solve the problems of insufficient iron intake, reduced chlorophyll synthesis, decreased biomass, etc., and achieves convenient, simple and diversified processing, promotes growth of iron absorption, and promotes iron. The effect of elemental absorption
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Embodiment 1
[0029] Embodiment 1: Under normal circumstances, foliar spraying Streptomyces sp. OsiSh-2, promoting rice growth
[0030] Experimental site: soil culture in greenhouse
[0031] Experimental crop: Rice (Indica 93-11).
[0032] Experimental treatment group: preparation Streptomyces sp. OsiSh-2 spore suspension at a concentration of 1 × 10 8 CFUmL -1 , adding 0.2% Tween 20 for treatment; water containing 0.2% Tween 20 was used as a control. The specific method for preparing the spore suspension is as follows:
[0033] (1) Inoculate the strain on ISP-2 solid medium and culture it statically in a constant temperature incubator at 30 °C for 14 days.
[0034] (2) Use an inoculation loop to collect the mature bacteria in step 1), transfer them to a conical flask containing sterile distilled water and glass beads, shake vigorously and then filter to obtain a spore suspension.
[0035] (3) Count the spore suspension obtained in step (2) with a hemocytometer to adjust its concentr...
Embodiment 2
[0041] Example 2: Under normal circumstances, Streptomyces sp. OsiSh-2 entraps rice seeds and promotes rice growth
[0042] Experimental conditions: Rice is grown hydroponically in a greenhouse constant temperature light incubator, the photoperiod is 16 hours of light / 8 hours of darkness, the cultivation temperature is 28-30°C, and the air humidity is 60-70%
[0043] Experimental crop: Rice (Indica 93-11).
[0044] Experimental treatment group: prepared according to the method of Example 1 Streptomyces sp. OsiSh-2 spore suspension at a concentration of 1 × 10 8 CFU mL -1 , the ratio of rice seeds / 0.3% (v / v) xanthan gum / spore suspension was 1g / 463 μl / 51 μl to embed the sterilized rice seeds, and rice seeds / 0.3% (v / v) The ratio of xanthan gum / sterile water is 1 g / 463 μl / 51 μl, as a control. Each treatment was repeated three times, and each group contained 48 rice seedlings. Three weeks after germination, the growth of rice was observed and its agronomic traits were ...
Embodiment 3
[0048] Embodiment 3: under iron deficiency situation, Streptomyces sp. OsiSh-2 entraps rice seeds to promote rice growth and iron absorption
[0049] Experimental conditions: Rice is grown hydroponically in a greenhouse constant temperature light incubator, the photoperiod is 16 hours of light / 8 hours of darkness, the cultivation temperature is 28-30°C, and the air humidity is 60-70%
[0050] Experimental crop: Rice (Indica 93-11).
[0051] Experimental treatment group: prepared according to the method of Example 1 Streptomyces sp. OsiSh-2 spore suspension at a concentration of 1 × 10 8 CFU mL -1 , the ratio of rice seeds / 0.3% (v / v) xanthan gum / spore suspension was 1g / 463 μl / 51 μl to embed the sterilized rice seeds, and rice seeds / 0.3% (v / v) The ratio of xanthan gum / sterile water was 1g / 463 μl / 51 μl, as a control, each treatment was repeated three times, and each group contained 48 rice seedlings. After germination, it was planted for one week under normal conditio...
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