Thermophilic streptomyces hygroscopicus cDNA library construction, and expression and expression methods of two greenhouse farmer's lungpathopoiesia antigen polypeptides
A technology of Streptomyces hygroscopicus and antigenic polypeptides, applied in recombinant DNA technology, chemical instruments and methods, botanical equipment and methods, etc., can solve problems such as aggravating the economic burden of sick farmers and social burden, increasing diagnosis and treatment costs, and delaying treatment timing.
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[0038] The constructed cDNA library of Streptomyces thermophilus hygroscopicus obtained 978 sense sequences and 347 single genes, among which the homology of one of the two genes to the outer membrane protein of Actinomyces pleuropneumoniae was 51%. The length of the open reading frame (ORF) was 1554 bp, encoding 517 amino acids; the homology of another gene with transferrin B was 42%, the length of the open reading frame (ORF) was 726 bp, encoding 241 amino acids.
[0039] The expression of the two greenhouse lung pathogenic antigen polypeptides is to subclone the two segments of the gene into a prokaryotic expression vector, and the relative molecular masses of the IPTG-induced expression products are 63,000 and 30,000 polypeptides, respectively.
[0040] The method for constructing cDNA library of Streptomyces thermophilus hygroscopicus and expressing two greenhouse lung pathogenic antigen polypeptides comprises the following steps:
[0041] 1. Construction of cDNA library ...
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