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Novel actinomycin A and preparation method and application thereof

A technology of actinomycin and marine actinomycetes, applied in the field of medical biology

Active Publication Date: 2017-06-30
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Involving the chemical structure, preparation method, semi-synthetic method, biosynthetic pathway, and anti-drug-resistant bacteria and anti-tumor activities of compound 1, there have been no relevant reports so far

Method used

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  • Novel actinomycin A and preparation method and application thereof
  • Novel actinomycin A and preparation method and application thereof
  • Novel actinomycin A and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Identification of Streptomyces sp. IMB094

[0067] a) Source of the strain: Streptomyces sp. IMB094 was isolated from marine sediments at a depth of about 40m in Heishijiao Bay (38°49'N, 121°34'E) in the Yellow Sea, Dalian, China in our laboratory.

[0068] b) Strain identification: Streptomyces sp. IMB094 was identified according to the conservation of the 16S rRNA gene sequence among microbial species. The genome of Streptomyces sp. IMB094 was extracted, its 16S rRNA gene was amplified by PCR and sequenced, and submitted to the NCBI GenBank database to obtain the accession number KY111376, and its sequence is shown in SEQ ID NO.1. Phylogenetic tree such as figure 1 shown.

[0069] SEQ ID NO.1

[0070]

[0071]

[0072] It shows that the bacterium belongs to a kind of Streptomyces in the genus Streptomyces, so the bacterium is named Streptomyces sp. Microbiology Center, deposit number: CGMCC 13680.

Embodiment 2

[0073] Fermentation of Streptomyces sp. IMB094

[0074] Take the activated streptomyces (Streptomyces sp.) IMB094 plate, pick about 1cm 2 Inoculate the bacterial lawn into 100mL fermentation medium [recipe: 1.0g starch, 2.5g glucose, 1.0g cottonseed cake powder, 0.3g peptone, inorganic salt such as KH 2 PO 4 0.01g, MgSO 4 0.01g, NaCl 0.5g, CaCO 3 0.5g, etc., deionized water 100mL], in a 500mL shake flask, 28 ℃, 200r / min shaker culture 120h.

Embodiment 3

[0075] The extraction of fermented liquid and the obtaining of extract

[0076] Streptomyces sp. IMB094 fermentation liquid was separated from solid and liquid to obtain filtrate and mycelium; mycelium was extracted with acetone ultrasonically to obtain mycelium acetone extract; the filtrate was adsorbed by XAD-7HP macroporous adsorption resin column, Sequentially eluted with water, 50%, 90% acetone; 50%, 90% acetone eluted part is combined with mycelia acetone extract, concentrated under reduced pressure to remove acetone to obtain fermented liquid extract.

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Abstract

The invention relates to a novel actinomycin A, a preparation and synthesis method thereof and an application of a compound to anti-drug-resistant bacteria and tumor resistance. The novel actinomycin A is a fermentation product of a marine streptomyces IMB094 (Streptomyces sp. IMB094). Experimental research results show that the novel actinomycin A has high antimicrobial activities for tested Gram-positive resistant bacteria and high inhibiting effects on tested tumor cells, and novel actinomycin A is expected to be novel clinical useful anti-drug-resistant bacteria and tumor-resistant medicines.

Description

[0001] Technical field: [0002] The invention belongs to the field of medical biology, and relates to a new structure actinomycin derivative new actinomycin A obtained from marine microorganisms; and a preparation method and pharmaceutical application thereof. [0003] Background technique: [0004] At present, bacterial drug resistance is becoming more and more serious, and the development of new antibiotics is imminent. Actinomycin (English name: (Actinomycins) is a class of pigment peptide antibiotics, composed of chromophore mother nucleus phenoxazinone chromophore linked to two pentalactone rings through amide bonds, has anti-tumor, Antibacterial, antiviral and antituberculous activities [Yuan Wei, Jiao Weihua, Wang Ying, et al. Research progress on actinomycin biosynthesis[J]. Amino Acids and Biological Resources, 2014,36(1):1-7; Anthony, B.M. ; Helmut, L.The Actinomycins.In Anticancer Agents from Natural Products, Second Edition, CRC Press: 2011; pp 363-382.] In recent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/02C12P21/02C12N1/20A61K38/08A61P35/00A61P31/04C12R1/465
CPCA61K38/00C07K7/02C12P21/02C12N1/205C12R2001/465
Inventor 甘茂罗王强王冕胡媛媛谭亿何红伟王以光肖春玲周红霞
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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