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A microbial agent against potato scab

A technology of potato scab and microbial inoculum, applied in agricultural and biological fields

Active Publication Date: 2016-02-10
DALIAN SANKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem to be solved by the present invention is to develop a microbial agent capable of efficiently antagonizing potato scab. Using the interaction between microorganisms, equipped with high-tech production fermentation technology to produce high-quality products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation of fermented liquid

[0021] 1) Inoculation: prepare solid culture medium of various strains, carry out strict aseptic operation after sterilization, transfer from inclined plane to flat plate, and cultivate at optimum temperature for 2 to 3 days;

[0022] 2) Primary culture: Pick a single colony from the well-grown plate and transfer it to a 50mL Erlenmeyer flask filled with primary liquid medium, perform aseptic operation, and culture at 30°C and 130r / min for 24-48 hours;

[0023] 3) Secondary fermentation: Inoculate the primary culture strains into a 500mL Erlenmeyer flask filled with secondary liquid medium, perform aseptic operation, and culture at 30°C and 130r / min for 24-48h to obtain Bacillus subtilis ( Bacillus subtilis) CGMCC1.3382 fermentation broth, actinomycete (Dactylosporangium sp.) ACCC No. 40661 fermentation broth, rose yellow streptomyces (Streptomyces roseoflavus) ACCC No. 40400 fermentation broth.

[0024] Medium used i...

Embodiment 2

[0028] Embodiment 2: Optimization of fermentation strain culture conditions

[0029] For the Bacillus subtilis (Bacillus subtilis) CGMCC1.3382 fermentation liquid obtained by culturing in Example 1, the actinomycete (Dactylosporangiumsp.) ACCCNo. , 30-35%, and 33-38% by mass percentage, inoculate the mixed bacterial solution into a fermenter filled with fermentation medium, the inoculum amount is 1-5% of the total fermentation medium mass, at 30°C Cultivate for 4 to 7 days, so that the number of bacteria in the fermentation broth reaches 10×10 8 individual / mL or more, the microbial bacterial agent for antagonizing potato scab can be obtained. Wherein, the fermentation medium is: glucose 15-30g / L, starch 10-15g / L, peptone 15-23g / L, NaCl5-10g / L, KH 2 PO 4 0.1~0.5g / L, the rest is distilled water; pH6.0~8.0.

[0030] For the fermentation medium used in embodiment 1, 1. the optimum carbon source, 2. the optimum carbon source concentration, 3. the optimum nitrogen source, and 4....

Embodiment 3

[0039] Embodiment 3: Strain compound and liquid submerged fermentation

[0040] The various strains are mixed according to the following mass percentages: 30% of Bacillus subtilis fermentation broth, 35% of actinomycetes fermentation broth, and 35% of Streptomyces roseus flavum fermentation broth. Inoculate the mixed bacterial liquid into a fermenter filled with fermentation medium, the inoculum amount is 3% of the total fermentation medium mass, and cultivate at 30°C for 5 days, so that the number of bacteria in the fermentation liquid reaches 10×10 8 individual / mL or more, the microbial bacterial agent for antagonizing potato scab can be obtained. Wherein, the fermentation medium is: glucose 15-30g / L, starch 10-15g / L, peptone 15-23g / L, NaCl5-10g / L, KH 2 PO 4 0.1~0.5g / L, the rest is distilled water; pH6.0~8.0.

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Abstract

The invention discloses a microbial agent which adopts bacillus subtilis CGMCC1. 3382, dactylosporangium sp. ACCC No. 40661 and streptomyces roseoflavus ACCC No. 40400 as preparation bacteria; all bacteria are prepared to form fermentation liquors, the fermentation liquors are mixed, and the mixture is subjected to submerged fermentation to prepare the microbial agent. The microbial agent utilizes the interaction among microorganism in a reasonable manner, has the characteristics of high adaptability, excellent stability, and high antagonism to pathogenic bacteria, and difficulty in aging, can realize efficient antagonism to the potato common scab, stimulate growth of the potatoes, and improve the quality of the potatoes so as to achieve the purposes of production increasing and high yield.

Description

technical field [0001] The invention belongs to the field of agriculture and biotechnology, and relates to a microbial agent for antagonizing potato scab, in particular, to a kind of effective compounding of different strains which can effectively treat potato scab and is produced by synergistic effect. A microbial agent. Background technique [0002] Potatoes are one of the most important crops in my country, second only to rice, wheat and corn, and are the fourth largest crops in my country. Potatoes are grown in a wide range of areas, and the diseases are relatively complex. Among them, potato scab caused by a variety of phytopathogenic Streptomyces is one of the important diseases affecting potato production today. [0003] Potato scab is mainly caused by Streptomyces, whose mycelium is slender and continuously divided to produce a large number of spores. The spores are cylindrical, and the pathogenic bacteria generally invade through the soil stomata from the potato e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/125C12R1/01C12R1/465
Inventor 顾艳丽崔国臣周悦崔昌娜
Owner DALIAN SANKE BIO ENG
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