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Method for preparing diglyceride

A technology of diglyceride and vegetable oil, applied in the direction of fermentation, etc., to achieve good industrial application prospect, good catalytic effect, and the effect of reducing product cost

Inactive Publication Date: 2008-04-09
JINAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the preparation of diglycerides by catalyzing the hydrolysis of animal oil or vegetable oil with phospholipase A1

Method used

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  • Method for preparing diglyceride
  • Method for preparing diglyceride
  • Method for preparing diglyceride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 100 g of refined soybean oil into a three-neck reactor, add 10 g of water and 0.05 g of phospholipase A1. The middle port of the three-neck reactor is connected to the stirrer, the left port is connected to the condenser, and the right port is closed with a ground plug. The reaction temperature was set at 50° C. and the stirring speed was 300 r / min for 4 hours. After the reaction, the reaction solution was poured into a separatory funnel and allowed to stand for 60 min to separate layers. The upper layer is an oil layer, and the lower layer is water and enzyme liquid. Drain the liquid in the lower layer, transfer the oil layer into a flask, and dry it by rotary evaporation for 30 minutes at an absolute pressure of 5000 Pa and a temperature of 90° C. to obtain crude diglyceride. The acid value of the crude diglyceride was determined to be 36.80 mgKOH / g.

[0029] Distill the crude diglycerides at 135°C, an absolute pressure of 30 Pa, and a stirring speed of 300 r...

Embodiment 2

[0031] Weigh 100 g of refined soybean oil into a three-neck reactor, add 36 g of water and 0.26 g of phospholipase A1. The middle port of the three-neck reactor is connected to the stirrer, the left port is connected to the condenser, and the right port is closed with a ground plug. The reaction temperature was set at 40° C. and the total stirring speed was 300 r / min to react for 8 hours. After the reaction, the reaction solution was poured into a separatory funnel and allowed to stand for 40 min to separate layers. The upper layer is an oil layer, and the lower layer is water and enzyme liquid. Drain the liquid in the lower layer, transfer the oil layer into a flask, and dry it by rotary evaporation for 40 minutes under an absolute pressure of 4000 Pa and a temperature of 80° C. to obtain crude diglyceride. The acid value of the crude diglyceride was determined to be 53.79 mgKOH / g.

[0032] Distill the crude diglyceride at 130°C, an absolute pressure of 20 Pa, and a stirri...

Embodiment 3

[0034] Weigh 100 g of refined peanut oil into a three-neck reactor, add 40 g of water and 0.18 g of phospholipase A1. The middle port of the three-neck reactor is connected to the stirrer, the left port is connected to the condenser, and the right port is closed with a ground plug. The reaction temperature was set at 30° C. and the total stirring speed was 300 r / min to react for 12 hours. After the reaction, the reaction solution was transferred to a centrifuge for centrifugation and layering. The upper layer is an oil layer, and the lower layer is water and enzyme liquid. Drain the liquid in the lower layer, transfer the oil layer into a flask, and dry it by rotary evaporation for 50 minutes at an absolute pressure of 6000 Pa and a temperature of 80° C. to obtain crude diglyceride. The acid value of the crude diglyceride was determined to be 53.36 mgKOH / g.

[0035] Distill the crude diglyceride at 140°C, absolute pressure 1Pa, and stirring speed 300r / min to remove the fatt...

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Abstract

The invention relates to a method for preparing functional fatty, in particular to a preparing method of diacylglycerol. The preparing method comprises the steps that: (1) 50 shares of animal oil or vegetable oil in weight, 5-30 shares of water in weight and 0.03-0.20 shares of phosphatidase A1 in weight are mixed and reacted for 2 to 12 hours under the temperature of 30 to 70 DEG C and stand for 1 to 2 hours or centrifugalize to delaminate; (2) the oil layer gained by delamination on the condition of temperature of 80 to 95 DEG C and absolute pressure of 4000 to 6000 Pa is dried for 30 to 60 minutes to get crude diacylglycerol. Purified diacylglycerol can be gained through the elimination of free fatty acids under the temperature of 120 to 160 DEG C and absolute pressure of 0.1 to 40 Pa. Phosphatidase A1 used by the invention has fine catalysis effect and the price is only 5 percent to 10 percent of fatty acid enzyme which leads to significant decrease of cost and fine application prospect in industry.

Description

technical field [0001] The invention relates to the preparation of functional oils, in particular to a method for preparing diglycerides. Background technique [0002] Diacylglyercrol (DAG) is a product obtained by esterifying glycerol with two fatty acids. There are mainly two isomers: 1,3-diglycerol and 1,2-diglycerol. Diacylglycerol is a natural component of oil, an important biological substance, a precursor for the synthesis of triglycerides, phospholipids and glycolipids, and an intermediate product of oil metabolism in the body. [0003] In recent years, many medical studies have shown that diglycerides have the functions of regulating blood lipids, preventing arterial thrombosis, inhibiting liver fat accumulation, relieving diabetic nephropathy, reducing weight and suppressing appetite. In 2001, ADM Corporation, the largest grain and oil processing company in the United States, and Japan's KAO (Kao) Corporation established a joint venture company, ADM KaoLLC. In 200...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62
Inventor 汪勇赵谋明欧仕益谢林云
Owner JINAN UNIVERSITY
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