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Protein for promoting plant growth and flowering and application of coding gene of protein

A technology for promoting plant growth and encoding genes, applied in the application field of proteins for promoting plant growth and flowering and their encoding genes

Inactive Publication Date: 2013-05-15
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on trehalose 6-phosphate synthase gene in rubber tree

Method used

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  • Protein for promoting plant growth and flowering and application of coding gene of protein
  • Protein for promoting plant growth and flowering and application of coding gene of protein
  • Protein for promoting plant growth and flowering and application of coding gene of protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1. Acquisition of related proteins and their coding genes for promoting plant growth and flowering of the present invention

[0033]Search the Hevea brasiliensis latex EST sequence database constructed by the applicant, determine a 492bp or so Hevea brasiliensis trehalose 6-phosphate synthase gene EST assembled sequence (contig), design a pair of specific primers to amplify the cDNA fragment, and Using rapid amplification of cDNA ends (RACE), the full-length cDNA sequence including the complete reading frame was finally obtained.

[0034] The specific method is as follows:

[0035] cDNA fragment cloning

[0036] Specific primers were designed as follows:

[0037] SP1 (5' end): 5'-GCCTTTTTCTTGGGTCCATTAT-3';

[0038] SP2 (3' end): 5'-GCCTCCAGCACTTATCTCCAAA-3'.

[0039] Using Hevea brasiliensis Reyan 7-33-97 (cultivated by the Rubber Research Institute of the Chinese Academy of Tropical Agricultural Sciences, and the Rubber Research Institute of the Chinese...

Embodiment 2

[0046] Example 2. Analysis of expression patterns of HbTPS2 gene in Hevea brasiliensis seedlings under abiotic stress

[0047] In this study, four-month-old Hevea brasiliensis Reyan 7-33-97 tissue-cultured seedlings were pre-adapted in nutrient solution for one month, and seedlings with similar growth conditions were selected for three abiotic stress treatments.

[0048] Low temperature stress: culture Hevea brasiliensis seedlings in nutrient solution at 4°C, and extract RNA from leaves, bark, xylem and roots at five time points of 0h, 3h, 6h, 12h and 24h respectively.

[0049] High temperature stress: culture Hevea brasiliensis seedlings in nutrient solution at 40°C, and extract RNA from four tissues of leaves, bark, xylem and roots at 0h, 3h, 6h, 12h, and 24h respectively.

[0050] Drought stress: Culture the Hevea brasiliensis seedlings in a nutrient solution containing 20% ​​PEG6000, and take leaves, bark, xylem and roots at 8 time points of 0h, 3h, 6h, 12h, 1d, 2d, 4d, an...

Embodiment 3

[0056] Example 3. Verification of yeast functional complementation of HbTPS2 gene

[0057] Preparation of yeast TPS gene mutant strain

[0058]Trp tag gene primers (Trp-TPS-F: 5'-CGTACACTTTCAAGTGGTTCGGATGGCCTGGGCTAGAGCACACAAAGG CAGCTTGGAG-3'; Trp-TPS-R: 5'-ACACTTCCATGGCGTGCAACTTCTGAGGC ACACCTTTAGTAATAACCTATTTCTTAGC-3') with TPS homology arms at both ends were designed, and the pGBKT7 plasmid (Baisheng Zang et al. Analysis of trehalose-6-phosphate synthase (TPS) gene family suggests the formation of TPS complexes in rice. Plant Molecular Biology. 2011, 76(6): 507-522. Rubber Institute of Chinese Academy of Tropical Agricultural Sciences Saved) was used as a template for PCR amplification to obtain a 706bp fragment.

[0059] The recovered fragment of the Trp tag gene with the homology arm was replaced by homologous recombination technology in yeast W303-1A (Baisheng Zang et al.Analysis of trehalose-6-phosphate synthase(TPS) gene family suggests the formation of TPS complexes i...

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Abstract

The invention discloses a protein for promoting plant growth and flowering and application of coding gene of protein. The protein is one of a protein which consists of an SEQ ID No.1 amino acid residue sequence in a sequence table and a protein which is obtained by substitution and / or deletion and / addition of one or more of amino acid residues of a SEQ ID No.1 amino acid residue sequence in the sequence table, has related functions of promoting plant growth and early flowering and is derived from SEQ ID NO: 1. The gene disclosed by the invention can be used as important target gene of high yield transgenic breeding of Hevea brasiliensis, the growth speed of the Hevea brasiliensis can be accelerated by regulating the expression of the gene.

Description

technical field [0001] The invention relates to a protein related to promoting plant growth and flowering derived from Hevea brasiliensis, its coding gene and application. Background technique [0002] The TPS / TPP pathway in plants is the pathway of trehalose synthesis. The process is first catalyzed by 6-phosphate trehalose synthase (Trehalose-6-phosphate synthase, TPS) to uridine diphosphate glucose (UDPG) and 6-phosphate glucose ( G-6-P) Synthesize the intermediate product trehalose 6-phosphate (T-6-P), and then generate the final product seaweed under the catalysis of 6-phosphate trehalose phosphatase (Trehalose-6-phosphate phosphatase, TPP) sugar. The intermediate product trehalose 6-phosphate (T-6-P) has a very important position in plants, and plays a role in signal transduction and regulation of plant growth and development. [0003] The research results of Vuorio O.E et al. (1993) showed that the intermediate product trehalose 6-phosphate (T-6-P) plays a decisive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/10C12N15/54C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10A01H5/00
Inventor 唐朝荣戚继燕周斌辉
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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