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Method for preparing diglyceride from phosphatidase A1 through catalytic esterification

A technology of diglyceride and catalyzed esterification, applied in the direction of fermentation, etc., to achieve the effect of cost advantage and good application prospect

Inactive Publication Date: 2010-09-29
JINAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the previous research, the inventor has reported the technology of phospholipase A1 (Lecitase Ultra) to catalyze the partial hydrolysis of soybean oil to prepare diglyceride products, but the hydrolysis method will produce about 30% of by-products - free fatty acids

Method used

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  • Method for preparing diglyceride from phosphatidase A1 through catalytic esterification
  • Method for preparing diglyceride from phosphatidase A1 through catalytic esterification

Examples

Experimental program
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Effect test

preparation example Construction

[0022] Preparation of reaction raw material fatty acid: Weigh 500.00g soybean oil in a 1000mL three-necked flask and react for 8h in a constant temperature water bath at 40℃ with a stirring speed of 300r / min to obtain a hydrolysate; the hydrolysate is allowed to stand for 30min to separate into two layers ; The upper layer is an oil layer containing glycerides and fatty acids. The oil layer is at 160°C and a pressure of 1.0 Pa to obtain hydrolyzed fatty acids (FFA) through molecular distillation.

[0023] The acid value of oil is determined according to GB / T 5530-1995 titration method.

[0024] The calculation method of the esterification rate is as follows: esterification rate=(initial fatty acid acid value-oil layer acid value after reaction) / initial fatty acid acid value×100%. HPLC method was used to determine the content of crude diglyceride and molecularly distilled diglyceride.

Embodiment 1

[0026] Put the fatty acid and glycerol molar ratio 0.5:1 in the sandwich beaker to obtain the substrate; add 80 U / g substrate phospholipase A1 (Lecitase Ultra) and the substrate mass 4.0wt% deionized water to the substrate in turn , The reaction temperature is controlled to 40℃ by super constant temperature water bath, and the magnetic stirrer is used to stir (rotation speed 300r / min). The reaction is carried out under vacuum (absolute pressure 400Pa), and the reaction time is 12h; after the enzymatic hydrolysis reaction is completed, the reaction solution is Centrifuge at 3000r / min for 10 minutes to separate layers, the lower layer is unreacted glycerin, and the upper layer is crude diglyceride (containing fatty acid impurities); the acid value of the upper layer is measured, the esterification rate is 43.87%, and the DAG content in the crude diglyceride is 12.78%.

[0027] The crude diglyceride layer obtained above is fed into a molecular distillation feed tank, and after dehyd...

Embodiment 2

[0029] Place the fatty acid and glycerol at a molar ratio of 2.21:1 in a sandwich beaker to obtain a substrate; add 90 U / g substrate phospholipase A1 (Lecitase Ultra) and a substrate mass of 4.0 wt% deionized water to the substrate in turn , The reaction temperature is controlled to 41 ℃ by super constant temperature water bath, and the magnetic stirrer is used to stir (rotation speed 300r / min). The reaction is carried out under vacuum (absolute pressure 2000Pa), and the reaction time is 12h; after the enzymatic hydrolysis reaction is completed, the reaction solution is Centrifuge at 3000r / min for 10 minutes to separate layers. The lower layer is unreacted glycerin, and the upper layer is crude diglyceride (containing fatty acid impurities); the acid value of the upper layer is measured, the esterification rate is 82.34%, and the DAG content in the crude diglyceride is 41.54%, the HPLC analysis chart is as figure 1 (a) Shown.

[0030] The crude diglyceride layer obtained above i...

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Abstract

The invention discloses a method for preparing diglyceride from phosphatidase A1 (Lecitase Ultra) through catalytic esterification, which comprises the following steps: (1) obtaining substrates after mixing fatty acid and glycerin according to a mol ratio of 0.5 / 1 to 2.5 / 1; and sequentially adding phosphatidase A1 and de-ionized water into the substrates to obtain reactant, wherein the addition of the phosphatidase A1 meets the requirement of adding 50 to 120 U phosphatidase A1 into each gram of the substrates, and the addition of the de-ionized water accounts for 4.0 to 12.0 wt percent of the mass of the substrates; (2) controlling the temperature between 30 and 55 DEG C under the condition that the reactant is in the vacuum, stirring the materials for reaction for 4 to 16h to obtain reaction liquid; and carrying out centrifugation delamination on the reaction liquid to obtain coarse diglyceride at the upper layer; and (3) carrying out molecular distillation on the coarse diglyceride under the conditions of the temperature between 160 and 165 DEG C and the pressure between 0.3 and 1.0 Pa to remove unreacted fatty acid and obtain the diglyceride.

Description

Technical field [0001] The invention belongs to the technical field of functional edible fats and oils, and particularly relates to a method for preparing diglycerides by catalyzing esterification with phospholipase A1. Background technique [0002] Diacylglycerol (DAG) is a product obtained by the esterification of two molecules of fatty acid with glycerol. It is a natural component of fats and oils and belongs to GRAS (Generally Recognized as Safe) substances. The energy values ​​of diglycerides and triglycerides are similar, but their absorption and metabolism modes in the organism are different. Oils with high diglyceride content as edible oils can inhibit obesity and reduce lifestyle-related diseases, so they have received widespread attention . The domestic research on diglycerides is still in the research and development stage, and there are no relevant commercial products. [0003] The preparation of diglycerides mainly includes chemical synthesis, enzymatic and biologica...

Claims

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Application Information

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IPC IPC(8): C12P7/64
Inventor 汪勇王丽丽胡长鹰赵谋明
Owner JINAN UNIVERSITY
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