The invention discloses a kit for detecting lipoprotein phospholipase A2 protein concentration. The kit for detecting the lipoprotein phospholipase A2 protein concentration comprises a calibrator, a cleaning solution, a substrate solution, a pretreatment solution, an enzyme conjugate working solution and a magnetic bead conjugate working solution. The pretreatment solution contains a surfactant, the enzyme conjugate working solution contains an enzyme labeled Lp-PLA2 antibody, and the magnetic bead conjugate working solution contains magnetic beads coated with the Lp-PLA2 antibody. According to the kit for detecting the lipoprotein phospholipase A2 protein concentration, chemiluminescent immunoassay is combined with magnetic particle separation technology, high detection sensitivity, highspecificity and accurate result are achieved, and wide range detection of 50-1000 ng / ml is achieved. Finger tip whole blood or anticoagulated venous whole blood is directly used as a sample to be detected, direct detecting can be carried out without pretreatment of a sample in advance, so that the detection speed is greatly improved, the operation steps are simplified, and the application range ofthe kit is expanded; the kit can be operated in a fully automatic and one-touch mode, and a test result can be obtained in about 7 minutes, the requirements of hospital emergency and outpatient rapiddiagnosis can be well met, and wide-scale popularization and application are facilitated.