Anti-phospholipase A2 acceptor antibody IgG kit and detection method

Abstract

The invention relates to an anti-phospholipase A2 acceptor antibody IgG kit and a detection method. The kit comprises a micropore board prewrapped with human phospholipase A2 receptor antigens, and the following bottled reagents: an anti-phospholipase A2 acceptor antibody IgG calibrator, a positive control group, a negative control group, a concentrated washing liquid, a sample diluent, a standard enzyme solution, a substrate liquid and a stop solution. According to the detection method of the invention, the contents of anti-phospholipase A2 acceptor antibody IgG in serum and plasma can be quantitatively detected in a non-invasive, low-risk, safe, cheap and precise manner, the positive detection rate of patients suffering from idiopathic membranous nephropathy is high, the detection result is precise and objective, the method is simple and convenient, and the popularization and the application are easy. The anti-phospholipase A2 acceptor antibody IgG kit and the detection method have important significances in diagnosis, treatment guidance and curative effect evaluation of idiopathic membranous nephropathy, and important clinical reference values in treatment scheme making and method selection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an enzyme-linked immunoassay kit and a detection method for quantitatively detecting anti-phospholipase A2 receptor antibody IgG in human serum and plasma. Background technique [0002] According to the etiology, membranous nephropathy can be divided into idiopathic and secondary membranous nephropathy. The diagnosis depends on clinical manifestations, histological examination of nephrocentesis or electron microscope examination of renal tissue. Renal puncture is an invasive diagnostic method that causes certain harm to the patient; electron microscope detection of kidney tissue requires high equipment and is difficult to be widely used. The autoimmune matrix of idiopathic membranous nephropathy was first reported abroad in 2009, which is essentially the result of the reaction between autoantibodies and phospholipase A2 receptors. Phospholipase A2 receptors are expressed on the surf...

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Problems solved by technology

Western blotting is still in the stage of laboratory research, mainly in the comparative test with histopathological detection methods, the sensitivity and specificity of the method are relatively low; indirect immunofluorescence, which uses transfected cells as a matrix to detect antiphospholipids Enzyme A2 receptor antibody, the results of positive samples have fluorescence, and negative samples do not, which is a qualitative detection method, and cannot accurately compare the antibody titers at different stages; the time-resolved fluorescence method is relatively complicated and requires specialized time-resolved fluorescence. Detection instruments, high cost of reagents, are in the research stage

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