43results about How to "High final concentration" patented technology

The invention provides methods for concentrating a macromolecule from a solution comprising the macromolecule and an organic polymer by first subjecting the solution to ultrafiltration to produce a first retentate solution, then adjusting the conductivity of the first retentate solution such that any protein precipitation induced by the organic polymer is essentially prevented to produce a second retentate solution, and then subjecting the second retentate solution to ultrafiltration. In a preferred embodiment, the conductivity is adjusted by diafiltration against water, suitable diluent or buffer. Preferably, the invention pertains to the concentration of solutions of native or recombinant proteins. The invention further pertains preferably to methods for the concentration of cell culture supernatant comprising a product protein and organic polymers of the Pluronic family of block co-polymers, and more preferably comprising Pluronic F-68 block co-polymer.

A process for preparing 1,3-propanediol biologically features that in the culture medium for fermenting or in the anaerobic fermenting procedure, appropriate reducer is added to increase the accumulation of reduction equivalent weight in thallus for promoting metabolism of glycerine along the reductino approach, and increasing the concentration and transform rate of 1,3-propanediol. Its advantageis high biologic utilization rate.

The invention discloses a genetic engineering strain for producing (R, R)-2,3-butanediol, as well as a construction method and an application thereof, and belongs to the field of genetic engineering. The genetic engineering strain is a genetic engineering strain obtained by transforming escherichia coli (Escherichia coli)MG1655 by a budB gene, a budA gene and a ydiL gene through an expression vector pTrc99a. The strain disclosed by the invention is capable of highly producing a high-purity(R,R)-2,3-butanediol in a case of not adding inductive agents and has the advantages of good cell growth, quick consumption of the substrate, short fermentation period and low cost at the same time. The engineering strain also has important industrial application value for the bio-manufacturing of the (R, R)-2,3-butanediol.

The invention belongs to the field of biochemical industry and discloses a 1,3-propanediol-producing genetically engineered bacterium as well as a preparation method and application thereof. The strain is named Klebsiella pneumoniae ATCC 25955-pUC18-yqhD-TetR, which is composed of the 1,3-propanediol oxidoreductase isoenzyme gene yqhD from Escherichia coli and the tetracycline resistance gene TetR from the plasmid pHY300PLK, and inserted into the vector pUC18 , obtained by transforming Klebsiella pneumoniae ATCC25955. The bacteria can significantly improve the ability of glycerol to convert 1,3-propanediol, improve the utilization ability and conversion rate of the substrate glycerol, increase the final concentration of the product 1,3-propanediol, and shorten the fermentation time, which is convenient for microbial fermentation. Industrial production of 1,3-propanediol.

The invention describes a method for producing or regenerating persulfuric acid and the salts thereof by electrolysis of an aqueous solution containing sulfuric acid and / or metal sulfates on diamond-coated electrodes without using promoters. According to the inventive method, bipolar silicon electrodes are used which are diamond-coated on one side and whose uncoated silicon back is used as the cathode.

The invention discloses a method for producing R-acetoin from klebsiella pneumoniae. Under an aerobiotic condition, the R-acetoin is produced from the klebsiella pneumoniae which is deactivated by an acetoin reductase gene budC through fermentation. The method provided by the invention has the advantages that the raw material is reproducible carbon source and the conversion rate of the raw material is high, the final concentration of the product R-acetoin is high, and the produced R-acetoin has very high optical purity and the content of R-isomers in the total acetoin is greater than 98%.

The invention provides methods for concentrating a macromolecule from a solution comprising the macromolecule and an organic polymer by first subjecting the solution to ultrafiltration to produce a first retentate solution, then adjusting the conductivity of the first retentate solution such that any protein precipitation induced by the organic polymer is essentially prevented to produce a second retentate solution, and then subjecting the second retentate solution to ultrafiltration. In a preferred embodiment, the conductivity is adjusted by diafiltration against water, suitable diluent or buffer.Preferably, the invention pertains to the concentration of solutions of native or recombinant proteins. The invention further pertains preferably to methods for the concentration of cell culture supernatant comprising a product protein and organic polymers of the PLURONIC family of nonionic block co-polymers, and more preferably comprising PLURONIC F-68 nonionic block co-polymer.

The present invention provides microbial aerobic fermentation process of producing 1, 3-propylene glycol, and belongs to the field of biochemical technology. Seed liquid is first added into industrial glycerin or initial fermentation culture medium of glycerin fermenting liquid with glycerin concentration of 20-50 g / L, and under fermentation condition of 30-40 deg.c, the culture medium is fermented with PDO producing Klebsiella pneumoniae or acid producing Klebsiella pneumoniae to produce PDO. During fermentation, air in 0.2-1.0 vvm is led in, the fermented matter is stirred in 50-250 rpm, glycerin and sodium hydroxide in certain amount are added to maintain certain glycerin concentration. The present invention has the advantages of lowered investment and power consumption, raised final PDO concentration, raised production strength and increased bacteria biomass.

A process for preparing 1,3-propanediol biologically features that in the culture medium for fermenting or in the anaerobic fermenting procedure, appropriate reducer is added to increase the accumulation of reduction equivalent weight in thallus for promoting metabolism of glycerine along the reductino approach, and increasing the concentration and transform rate of 1,3-propanediol. Its advantage is high biologic utilization rate.

The invention provides a preparation method of rebaudioside A, which comprises: preparing a reaction liquid by using a stevioside raw material as a substrate in the presence of uridine diphosphate glucose and glucosyltransferase, and adjusting the pH of the reaction solution to 6.0-8.0, after stirring reaction at a constant temperature of 20-45 DEG C, collecting rebaudioside A; the stevioside rawmaterial comprises stevioside, and the amino acid sequence of the glucosyltransferase comprises SEQ ID NO: 1 The amino acid sequence shown. The preparation method is a high-efficiency, simple, low-cost, high conversion rate, green and environmentally friendly biological enzymatic method, and can be widely applied to industrial scale production. The invention also provides an enzyme and applicationfor the preparation of rebaudioside A.

The invention relates to the field of microbial technology. It discloses a method to apply the cloud point system (CPS) in biotransformation by selecting one or more types of nonionic surfactant to form a aqueous system with a cloud point below the microbial transformation temperature, which serves as transformation medium. The method disclosed is suitable in particular for microbial transformation of hydrophobic compounds, for the system where substrate or product inhibits microbial growth or where product is further degraded by microbes. The CPS in the present invention forms a microemulsion of water-in-oil and oil-in-water, where the drops of surfactant is able to solubilize, serving as substrate reservoir and product extractant. This enhances bioavailability of substrates and elimination of product inhibition. The large water vesicles existing in the continuous surfactant phase provide aqueous environment to the cells where they can be sheltered from detrimental effects of surfactants, resulting in improvement of biocompatibililty.

The invention relates to an SDS-PAGE separation method for high-and-low-molecular-weight glutenin subunits in wheat. The SDS-PAGE separation method comprises the following steps: removal of alcohol-soluble proteins; reduction of glutenin; mixed alkylation of glutenin subunits and a sample buffer; and SDS-PAGE. The method of the invention reduces the usage amount of a reagent, omits the preparationof a sample and shortens sample preparation time on the premise of maintaining sample separation effect.

The invention discloses a method and equipment for separating volatile organic matters from a fermented product. In the method, in-situ separation of the volatile organic matters is realized by coupling fermentation with a gas stripping / vapor permeation process. The method and the equipment are characterized in that: inert gas is introduced from a gas distributor at the bottom of a fermentation tank; in-situ gas is extracted from the volatile organic matters and enters a vapor permeation device so as to separate water from the organic matters; dehydrated mixed gas enters a condenser to recycle the volatile organic matters; and non-condensable gas and a water vapor component containing a small number of organic matters can be recycled. The method and the equipment have the advantages of eliminating product inhibition, enhancing fermentation intensity, saving water and reducing energy consumption along with high in-situ movement efficiency of the volatile organic matters in a fermentation process. The volatile organic matters in the invention comprise ethanol, propanol, acetone, butanol and the like.

Disclosed is a process for treating a gas stream containing hydrochloric acid, hydrofluoric acid, a fluorinated compound and halogenated organic compounds, wherein the gas stream is subjected to: (a) a step of washing with an acid solution to obtain a washed gas stream; (b) a step of adiabatic absorption in an aqueous solution of the hydrochloric acid contained in said washed gas stream, to collect a solution of hydrochloric acid; (c) a step of adsorption on activated carbon of the impurities present in said hydrochloric acid solution, to obtain a purified hydrochloric acid solution and a gas stream containing said fluorinated compound; and (d) a step of bringing said purified hydrochloric acid solution into contact with a silica gel. Also disclosed is a plant for the implementation of this process, and also a process for preparing a fluorinated compound comprising the catalytic pyrolysis of an organofluorine compound.

The invention discloses a method for rapidly extracting total RNA from lotus root tissue. The steps are: first, the preparation of experimental medicines and the treatment of experimental supplies; second, the collection of materials, the young leaves of lotus root and the petioles connected with the leaves; the third, extraction, precipitation, and centrifugation to obtain total RNA; the fourth is The identification of total RNA was detected by UV spectrophotometer and formaldehyde-denatured agarose gel electrophoresis respectively. According to the obtained data and electrophoresis results, it was shown that the obtained lotus root tissue total RNA had good integrity, high purity and high yield. The invention has the advantages of simple and convenient experimental process, convenient and safe operation, accurate and effective results and good repeatability, and the method is also suitable for extracting total RNA from plant tissues of other species rich in polysaccharides and polyphenols.

The invention provides a recombined saccharomyces cerevisiae strain for producing ethyl alcohol, a construction method of the recombined saccharomyces cerevisiae strain and a method for producing ethyl alcohol through the recombined saccharomyces cerevisiae strain. According to the recombined saccharomyces cerevisiae strain for producing ethyl alcohol, a wild type saccharomyces cerevisiae strain is used as an original strain, an XI xylose metabolism pathway is introduced into the original strain, the expression of four key genes in a PPP pathway is enhanced, meanwhile, the aldose reductase gene GRE3 is knocked out, the nitrobenzene phosphatase gene PHO13 is optionally knocked out, and XI protein and XK protein introduced exogenously are positioned to a yeast cell membrane through the membrane positioning effect of a membrane positioning group. The recombined saccharomyces cerevisiae strain for producing ethyl alcohol can efficiently carry out xylose metabolism, so that co-fermentation of C5 sugar and C6 sugar is achieved, and therefore the higher ethyl alcohol conversion rate is achieved.