43results about How to "High final concentration" patented technology

The invention discloses a genetic engineering strain for producing (R, R)-2,3-butanediol, as well as a construction method and an application thereof, and belongs to the field of genetic engineering. The genetic engineering strain is a genetic engineering strain obtained by transforming escherichia coli (Escherichia coli)MG1655 by a budB gene, a budA gene and a ydiL gene through an expression vector pTrc99a. The strain disclosed by the invention is capable of highly producing a high-purity(R,R)-2,3-butanediol in a case of not adding inductive agents and has the advantages of good cell growth, quick consumption of the substrate, short fermentation period and low cost at the same time. The engineering strain also has important industrial application value for the bio-manufacturing of the (R, R)-2,3-butanediol.

The invention belongs to the field of biochemical industry and discloses a 1,3-propanediol-producing genetically engineered bacterium as well as a preparation method and application thereof. The strain is named Klebsiella pneumoniae ATCC 25955-pUC18-yqhD-TetR, which is composed of the 1,3-propanediol oxidoreductase isoenzyme gene yqhD from Escherichia coli and the tetracycline resistance gene TetR from the plasmid pHY300PLK, and inserted into the vector pUC18 , obtained by transforming Klebsiella pneumoniae ATCC25955. The bacteria can significantly improve the ability of glycerol to convert 1,3-propanediol, improve the utilization ability and conversion rate of the substrate glycerol, increase the final concentration of the product 1,3-propanediol, and shorten the fermentation time, which is convenient for microbial fermentation. Industrial production of 1,3-propanediol.

The invention describes a method for producing or regenerating persulfuric acid and the salts thereof by electrolysis of an aqueous solution containing sulfuric acid and / or metal sulfates on diamond-coated electrodes without using promoters. According to the inventive method, bipolar silicon electrodes are used which are diamond-coated on one side and whose uncoated silicon back is used as the cathode.

The invention discloses a method for producing R-acetoin from klebsiella pneumoniae. Under an aerobiotic condition, the R-acetoin is produced from the klebsiella pneumoniae which is deactivated by an acetoin reductase gene budC through fermentation. The method provided by the invention has the advantages that the raw material is reproducible carbon source and the conversion rate of the raw material is high, the final concentration of the product R-acetoin is high, and the produced R-acetoin has very high optical purity and the content of R-isomers in the total acetoin is greater than 98%.

The present invention provides microbial aerobic fermentation process of producing 1, 3-propylene glycol, and belongs to the field of biochemical technology. Seed liquid is first added into industrial glycerin or initial fermentation culture medium of glycerin fermenting liquid with glycerin concentration of 20-50 g / L, and under fermentation condition of 30-40 deg.c, the culture medium is fermented with PDO producing Klebsiella pneumoniae or acid producing Klebsiella pneumoniae to produce PDO. During fermentation, air in 0.2-1.0 vvm is led in, the fermented matter is stirred in 50-250 rpm, glycerin and sodium hydroxide in certain amount are added to maintain certain glycerin concentration. The present invention has the advantages of lowered investment and power consumption, raised final PDO concentration, raised production strength and increased bacteria biomass.

A process for preparing 1,3-propanediol biologically features that in the culture medium for fermenting or in the anaerobic fermenting procedure, appropriate reducer is added to increase the accumulation of reduction equivalent weight in thallus for promoting metabolism of glycerine along the reductino approach, and increasing the concentration and transform rate of 1,3-propanediol. Its advantage is high biologic utilization rate.

The invention relates to an SDS-PAGE separation method for high-and-low-molecular-weight glutenin subunits in wheat. The SDS-PAGE separation method comprises the following steps: removal of alcohol-soluble proteins; reduction of glutenin; mixed alkylation of glutenin subunits and a sample buffer; and SDS-PAGE. The method of the invention reduces the usage amount of a reagent, omits the preparationof a sample and shortens sample preparation time on the premise of maintaining sample separation effect.

The invention discloses a method and equipment for separating volatile organic matters from a fermented product. In the method, in-situ separation of the volatile organic matters is realized by coupling fermentation with a gas stripping / vapor permeation process. The method and the equipment are characterized in that: inert gas is introduced from a gas distributor at the bottom of a fermentation tank; in-situ gas is extracted from the volatile organic matters and enters a vapor permeation device so as to separate water from the organic matters; dehydrated mixed gas enters a condenser to recycle the volatile organic matters; and non-condensable gas and a water vapor component containing a small number of organic matters can be recycled. The method and the equipment have the advantages of eliminating product inhibition, enhancing fermentation intensity, saving water and reducing energy consumption along with high in-situ movement efficiency of the volatile organic matters in a fermentation process. The volatile organic matters in the invention comprise ethanol, propanol, acetone, butanol and the like.

Disclosed is a process for treating a gas stream containing hydrochloric acid, hydrofluoric acid, a fluorinated compound and halogenated organic compounds, wherein the gas stream is subjected to: (a) a step of washing with an acid solution to obtain a washed gas stream; (b) a step of adiabatic absorption in an aqueous solution of the hydrochloric acid contained in said washed gas stream, to collect a solution of hydrochloric acid; (c) a step of adsorption on activated carbon of the impurities present in said hydrochloric acid solution, to obtain a purified hydrochloric acid solution and a gas stream containing said fluorinated compound; and (d) a step of bringing said purified hydrochloric acid solution into contact with a silica gel. Also disclosed is a plant for the implementation of this process, and also a process for preparing a fluorinated compound comprising the catalytic pyrolysis of an organofluorine compound.

The invention discloses a method for rapidly extracting total RNA from lotus root tissue. The steps are: first, the preparation of experimental medicines and the treatment of experimental supplies; second, the collection of materials, the young leaves of lotus root and the petioles connected with the leaves; the third, extraction, precipitation, and centrifugation to obtain total RNA; the fourth is The identification of total RNA was detected by UV spectrophotometer and formaldehyde-denatured agarose gel electrophoresis respectively. According to the obtained data and electrophoresis results, it was shown that the obtained lotus root tissue total RNA had good integrity, high purity and high yield. The invention has the advantages of simple and convenient experimental process, convenient and safe operation, accurate and effective results and good repeatability, and the method is also suitable for extracting total RNA from plant tissues of other species rich in polysaccharides and polyphenols.