The invention discloses a genetic engineering strain for producing (R, R)-2,3-butanediol, as well as a construction method and an application thereof, and belongs to the field of genetic engineering. The genetic engineering strain is a genetic engineering strain obtained by transforming escherichia coli (Escherichia coli)MG1655 by a budB gene, a budA gene and a ydiL gene through an expression vector pTrc99a. The strain disclosed by the invention is capable of highly producing a high-purity(R,R)-2,3-butanediol in a case of not adding inductive agents and has the advantages of good cell growth, quick consumption of the substrate, short fermentation period and low cost at the same time. The engineering strain also has important industrial application value for the bio-manufacturing of the (R, R)-2,3-butanediol.