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Peptide fractions promoting growth and synthesis of desired product(s) into cell and/or tissue culture

A tissue culture medium and cell technology, applied in the field of supplementing cells or tissue culture medium, can solve the problem of high cost, and achieve the effect of improving the production rate, high nitrogen raw material content, and improving the final concentration

Inactive Publication Date: 2007-12-05
PIERRE FABRE MEDICAMENT SAS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of obtaining these media is usually relatively high, and they are usually derived from raw materials containing high protein content, which can also be used in other applications, such as food use

Method used

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  • Peptide fractions promoting growth and synthesis of desired product(s) into cell and/or tissue culture
  • Peptide fractions promoting growth and synthesis of desired product(s) into cell and/or tissue culture
  • Peptide fractions promoting growth and synthesis of desired product(s) into cell and/or tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0126] Example 1: Scheme for the method of preparing the peptide extract of the invention

[0127] Figure 1 depicts the scheme described below. More particularly, this example restates all the steps according to a preferred embodiment, describing the details of the protocol. It will be appreciated that various changes in this method will be apparent to those skilled in the art.

[0128] Step 1: Protocol for Extracting Rapeseed Protein

[0129] The protocol for carrying out this step was carried out in the manner described below.

[0130] 30kg deoiled rapeseed cake + 300L 0.2M sodium hydroxide

[0131] |

[0132] Turbine mixer (30min, room temperature)

[0133] ↓

[0134] Cake / Alkaline Extract Blend

[0135] |

[0136] Horizontal spiral decanter centrifuge (3500g)

[0137] ↓

[0138] Extracts were collected and adjusted to pH 4 with hydrochloric aci...

Embodiment 2

[0170] Example 2: Protocol for identifying target extracts of the present invention

[0171] 2.1 Analysis of nitrogen by Kjeldahl method

[0172] ·Reagents

[0173] Mineralization catalyst (17%Na 2 SO 4 ; 1.5% CuSO 4 ·5H 2 O; 1.5% salt): Prolabo, 22550-293

[0174] -1N H 2 SO 4 : Labosi, A4715891

[0175] -35%H 2 o 2 : Labosi, A4823251

[0176] -40% NaOH: Merck, 191537

[0177] -H 3 BO 3 : Labosi, A4703851

[0178] ·raw material

[0179] - Vapodest 4 titramatic automatic Kjeldahl apparatus: Gerhardt GmbH & Co. KG, Bonn, Germany.

[0180] - 645 Multi-dosimat automatic titrator: Metrohm, Herisau, Switzerland.

[0181] -Kjeldatherm _ KT 12 S mineralized bricks: Gerhardt GmbH & Co. KG, Bonn, Germany.

[0182] ·Detection plan

[0183] Protein content was determined by Kjeldahl method. The principle of the determination method is: convert organic nitrogen into ammonium sulfate (NH 4 ) 2 SO 4 form of inorganic nitrogen. Detection is performed automatically ...

Embodiment 3

[0258] Embodiment 3: the effect of sterilization filtration

[0259] The culture system used consisted of 125ml Erlenmeyer flasks, Vu = 25ml.

[0260] CHO-C5 cells were cultured in the control medium (○) or the desired medium (△), and the medium was sterilized by 0.22um filter once (open type) or 3 times (solid type).

[0261] The results obtained are shown in Figure 2.

[0262] From the latter it can be seen that the effect of the peptide extract according to the invention is not reduced when subjected to 0.22um filtration.

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Abstract

The invention relates to preparing and / or supplementing a cell or tissue culture medium. In particular, said invention relates to a serum-free and / or protein-free cell culture medium comprising peptide fractions isolated from rapeseeds, in particular rapeseeds cakes. A method for the production of a cell culture comprising said peptide fractions and for the use thereof is also disclosed.

Description

technical field [0001] The present invention relates to the field of supplementing cell or tissue culture media. More particularly, the present invention relates to a serum-free and / or protein-free cell culture medium containing a peptide fraction isolated from rapeseed, in particular from rapeseed cake. The invention also relates to a method for preparing cell culture media containing these peptide components and their use. Background technique [0002] Since the existence of living cells has become known, the types and techniques of cell culture media have continued to advance and diversify. [0003] Initially, undefined culture media such as plasma, serum or embryonic extracts were used for cell culture. It wasn't until the mid-1950s that an initially defined culture medium contained, in addition to salt and glucose, various amino acids and vitamins that the cells could not synthesize themselves. [0004] It has recently been found that among all amino acids, L-glutami...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12N5/02C12N5/06C12N5/07
CPCC12N2500/99C07K14/57C12P21/06C12N5/0018C12N2500/76C12N1/04C12N2500/92C12N5/00C07K14/415
Inventor A·马克J-L·格尔根B·法尔热-哈丹尼
Owner PIERRE FABRE MEDICAMENT SAS
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