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Method of adding reducing agent from extraneous sources for accelerating thallus to synthesize 1,3-propylene glycol

A technology of reducing agent and propylene glycol, applied in 1 field, can solve the problems that have not been seen in reports, achieve high product synthesis concentration and conversion rate, improve bioavailability, and reduce fermentation cost

Inactive Publication Date: 2004-12-29
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current literature, there are many literatures on the research on the metabolic pathway of the 1,3-propanediol biotransformation process, but the report of applying the metabolic network analysis to the actual process research has not been seen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 uses Vitamin C as reducing agent

[0014] Species: Klebsiella pneumoniae

[0015] culture medium

[0016] LB solid medium: every liter of medium contains 5.0g of yeast powder, 10.0g of peptone, 10.0g of NaCl, 15.0g of agar, pH7.0.

[0017] Seed medium: 20g of glucose per liter volume of medium, K 2 HPO 4 .3H 2 O 4.45g, KH 2 PO 4 1.3g, (NH 4 ) 2 SO 4 2.0g, MgSO 4 .7H 2 O 0.2g, CaCO 3 2.0g, yeast powder 1.0g, trace element solution I 2.0ml, Fe 2+ Solution 1.0ml, pH7.0.

[0018] Fermentation medium: 20g of glycerol per liter volume of medium, K 2 HPO 4 .3H 2 O 4.45g, KH 2 PO 4 1.3g, (NH 4 ) 2 SO 4 2.0g, MgSO 4 .7H 2 O 0.2g, CaCO 3 2.0g, yeast powder 1.0g, trace element solution I 5.0ml, Fe 2+ Solution 1.0ml, pH7.0. Add reducing agent Vitamin C after sterilization, make the concentration of Vitamin C in fermentation medium be 40mg / L.

[0019] Among them, the composition of trace element I (mg / L): MgSO 4 .4H 2 O 100, ZnCl 2 7...

Embodiment 2

[0032] Embodiment 2 is at the beginning of the exponential phase (about 5 hours, OD) of thalline growth in fermentation process 650 0.5) add VC reducing agent, do not add reducing agent in fermentation medium, other conditions remain unchanged;

Embodiment 3

[0033] Embodiment 3 is the middle and late period (10-20 hours, OD 650 1.5-2.0) Add VC reducing agent, do not add reducing agent in the fermentation medium, and other conditions remain unchanged;

[0034] Program

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1,3-Propane

alcohol co...

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PUM

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Abstract

A process for preparing 1,3-propanediol biologically features that in the culture medium for fermenting or in the anaerobic fermenting procedure, appropriate reducer is added to increase the accumulation of reduction equivalent weight in thallus for promoting metabolism of glycerine along the reductino approach, and increasing the concentration and transform rate of 1,3-propanediol. Its advantage is high biologic utilization rate.

Description

Technical field: [0001] The invention relates to a method for adding a reducing agent from an external source to promote the synthesis of 1,3-propanediol by bacteria, belonging to the technical field of biosynthesis of 1,3-propanediol. Background technique: [0002] 1,3-propanediol (English name 1,3-propanediol, referred to as 1,3-PD) is an important chemical raw material, which can be used as an organic solvent in ink, printing and dyeing, coating, lubricant, antifreeze and other industries. [0003] The production technology of 1,3-PD is difficult and costly. At present, chemical synthesis and biosynthesis are mainly used in industry to produce 1,3-propanediol. The chemical synthesis method has the disadvantages of polluting the environment and non-renewable raw materials. Therefore, all countries are devoting themselves to the development of biosynthesis to produce 1,3-PD technology. The biosynthesis of 1,3-propanediol is a method with broad application prospects. It can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18
Inventor 李春张延平曹竹安
Owner TSINGHUA UNIV
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