The invention discloses a method for fermenting and preparing
citric acid by adding saccharifying
enzyme and belongs to the technical field of
fermentation engineering. In the traditional
citric acid fermentation, a method of mixing water and
starch raw materials for size mixing and adding
amylase so as to liquefy, heat, sterilize and ferment the raw materials is adopted, and in the
fermentation process, saccharifying
enzyme secreted by a production strain
aspergillus niger per se is saccharified and fermented simultaneously. The method has the disadvantages that: a time process is needed for the
aspergillus niger to grow and secrete saccharifying
enzyme; and the acidity is continuously increased along with the increase of the produced acid, and the saccharifying enzyme of the
aspergillus niger per se is gradually inhibited and cannot play a role in saccharifying normally at the later stage of the fermentation, so that the residual
sugar content in the fermented liquor of the
citric acid is higher in the end. The citric acid is prepared by the following steps of: adding the
amylase to liquefy the
starch raw materials, and heating for sterilization; adding a proper amount of saccharifying enzyme, and fermenting, filtering, decoloring, exchanging ions, separating, crystallizing and performing other processes according to the conventional technology. The method for fermenting and preparing the citric acid has the advantages of reducing
raw material consumption, improving the conversion rate of the raw materials, improving the acid-producing rate, shortening the fermentation time and reducing CODCr
discharge of
wastewater.