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Amino acid diagnosis/measurement reagent (kit) and amino acid concentration measurement method

An amino acid and reagent technology, applied in the field of amino acid concentration determination and amino acid diagnosis/determination reagents, can solve the problems of poor specificity, complicated operation methods, and high cost of instruments and equipment

Inactive Publication Date: 2010-06-23
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing determination methods include methods such as chromatography, electrochemical method, instrumental analysis method (amino acid analyzer), spectrophotometer, etc., and these methods have disadvantages such as comparatively complicated operation methods, poor specificity, or high cost of instruments and equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The amino acid diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0045] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0046] Stabilizer 500mmol / L

[0047] Reduced coenzyme 0.25mmol / L

[0048] Amino acid oxidase 6000U / L

[0049] Pyruvate oxidase 8000U / L

[0050] Taurine Opin Dehydrogenase 10000U / L

[0051] Sodium bicarbonate 6mmol / L

[0052] Acetyl phosphate 12mmol / L

[0053] Taurine 12mmol / L

[0054] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0055] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test subwavelength 405nm, the volume ratio of the amino acid sample to be tested is 1 / 25, The reaction direction is negative reaction (decreasing reaction), the delay time is about...

Embodiment 2

[0057] The amino acid diagnosis / measurement reagent of this embodiment is a double reagent, including:

[0058] Reagent 1

[0059] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0060] Stabilizer 50mmol / L

[0061] Reduced coenzyme 0.25mmol / L

[0062] Sodium bicarbonate 6mmol / L

[0063] Acetyl phosphate 12mmol / L

[0064] Taurine 12mmol / L

[0065] Reagent 2

[0066] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0067]Stabilizer 500mmol / L

[0068] Amino acid oxidase 6000U / L

[0069] Pyruvate oxidase 8000U / L

[0070] Taurine Opin Dehydrogenase 10000U / L

[0071] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0072] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the amino acid sample to ...

Embodiment 3

[0075] The amino acid diagnosis / measurement reagents of this embodiment are three reagents, including:

[0076] Reagent 1

[0077] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0078] Stabilizer 50mmol / L

[0079] Reduced coenzyme 0.25mmol / L

[0080] Sodium bicarbonate 6mmol / L

[0081] Acetyl phosphate 12mmol / L

[0082] Taurine 12mmol / L

[0083] Reagent 2

[0084] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0085] Stabilizer 500mmol / L

[0086] Pyruvate oxidase 8000U / L

[0087] Taurine Opin Dehydrogenase 10000U / L

[0088] Reagent 3

[0089] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0090] Stabilizer 500mmol / L

[0091] Amino acid oxidase 6000U / L

[0092] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0093] When measuring amino acid concentration, set on the automatic biochemical analyzer: temperatu...

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Abstract

The invention relates to an amino acid diagnosis / measurement reagent (kit) utilizing an enzyme-colorimetry method and an enzyme immunoassay technology, and relates to a method for measuring the concentration of amino acid as well as the composition and the components of the reagent, belonging to the technical field of medical / food / environmental detection and measurement. The reagent (kit) mainly comprises the components of buffer solution, NADH, sodium bicarbonate (carbon dioxide), acetyl phosphate, taurine, amino acid oxidase, pyruvic oxidase, taurine dehydrogenase and a stabilizer. The concentration of amino acid is measured by the steps of mixing a sample with the reagent in a certain volume radio to carry out a series of enzymatic reaction, placing the reactants under an ultraviolet / visible light analyzer, and detecting the reducing degree of absorbance at the main wavelength of 340 nm.

Description

technical field [0001] The invention relates to an amino acid diagnosis / determination reagent (box), and simultaneously the invention also relates to a method for determining amino acid concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] The determination of amino acid content is an important determination item in medicine / food / industry / agriculture / environment. Existing determination methods include chromatography, electrochemical method, instrumental analysis method (amino acid analyzer), spectrophotometer and other methods. These methods have disadvantages such as relatively complicated operation methods, poor specificity, or high equipment costs. [0003] Amino acid is not a simple substance, 17 kinds of amino acids can be directly measured with an amino acid analyzer (the instrument is expensive and cannot be widely used). For medicine / food / industry / agriculture / environment, in most...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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